Overview

Also Known As:BLC-

These Cxcl13 knock-out mice exhibit defective B cell organization, and are suitable for use in applications related to lymph node and Peyer's patch development.

Donating Investigator

Jason Cyster, University of California San Francisco

Genetic overview

Genetic Background	Generation

Cxcl13^tm1Cys

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Cxcl13	chemokine (C-X-C motif) ligand 13

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Research Applications

Developmental Biology Research
Immunology, Inflammation and Autoimmunity Research
Research Tools
Internal/Organ Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. No endogenous gene product (mRNA or protein) is detected in spleen or mesenteric lymph node. EGFP is not expressed. Severe, but incompletely penetrant, defects of the inguinal, iliac, axillary, brachial, popliteal, deep cervical, renal, sacral, and parathymic lymph nodes are observed. The lymphoid patch of the cecum is absent and Peyer's patches are severely reduced and typically malformed. This mutant has defective B cell organization, an absence of primary follicle follicular dendritic cells, and reduced B cell LTa1b2 expression in spleen and lymph nodes. This mutant may be useful in B cell, follicular dendritic cell, and/or lymph node development studies.

Development

A targeting vector was constructed in which base pairs 18-116 of exon 2 from the endogenous gene were replaced with an in-frame stop codon, a Mengo virus internal ribosome entry site, an enhanced green fluorescent protein gene (EGFP), and a loxP-flanked neomycin resistance gene. The construct was electroporated into 129X1/SvJ derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were backcrossed for germ-line transmission to C57BL/6J females. Offspring were mated with Cre-expressing C57BL/6J to remove the neomycin resistance gene. The neo-excised heterozygotes were backcrossed to C57BL/6J for ten generations before being made homozygous.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Ansel KM; Ngo VN; Hyman PL; Luther SA; Forster R; Sedgwick JD; Browning JL; Lipp M; Cyster JG. 2000. A chemokine-driven positive feedback loop organizes lymphoid follicles. Nature 406(6793):309-14PubMed: 10917533 MGI: J:78282

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Genetics

Cxcl13^tm1Cys

Allele Symbol: Cxcl13^tm1Cys

Allele Name	targeted mutation 1, Jason G Cyster
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	BLC^-; CXCL13^-
Gene Symbol and Name	Cxcl13, chemokine (C-X-C motif) ligand 13
Gene Synonym(s)
Strain of Origin	129X1/SvJ
Chromosome	5
Molecular Note	Base pairs 18 through 116 (exon 2) were replaced by a cassette containing a stop codon followed by an internal ribosome entry site, an enhanced green fluorescent protein gene, and a floxed neo gene. The deleted region encoded amino acids 27 through 60, including 3 of the 4 conserved Cys residues. The absence of transcript and protein in homozygous mutant mice was confirmed by Northern and Western blot analyses of homozygous mutant mice.
Mutations Made By	Jason Cyster, University of California San Francisco

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Cxcl13^tm1Cys related

Developmental Biology Research
- Lymphoid Tissue Defects
- Internal/Organ Defects
  - Lymphoid Tissue Defects
Immunology, Inflammation and Autoimmunity Research
- Lymphoid Tissue Defects
  - Lymphocyte Homing
  - selective lymph node development defects
- Immunodeficiency
  - B cell defects
Internal/Organ Research
- Lymphoid Tissue Defects

Immunology, Inflammation and Autoimmunity Research
- Lymphoid Tissue Defects
  - hematopoietic development
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - B cell deficiency
Developmental Biology Research
- Internal/Organ Defects
  - Lymphoid Tissue Defects
- Lymphoid Tissue Defects
  - hematopoietic defects
Internal/Organ Research
- Lymphoid Tissue Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Cxcl13^tm1Cys/Cxcl13^tm1Cys
involves: 129X1/SvJ * C57BL/6

immune system phenotype

abnormal spleen B cell follicle morphology
- B cells fail to organize into polarized follicular clusters and appear as a ring of cells at the perimeter of T-cell areas
- boundary between B-cell rich areas and T zones are often poorly demarcated, with increased numbers of B cells and T cells in reciprocal areas

absent follicular dendritic cells
- absence of primary follicular dendritic cells in spleen and lymph nodes

decreased spleen germinal center size
- germinal centers form in spleen after immunization with T-cell dependent antigen, however they are misplaced and smaller

abnormal spleen marginal zone morphology
- spleen shows a thickened ring of IgM^hiIgD^lo marginal zone B cells and the boundary between the B cells in this area and the inner ring of B cells is disrupted, with increased numbers of IgM^hiIgD^lo cells in the outer marginal zone
- outer marginal zone

absent axillary lymph nodes
- in most mice

decreased lymph node number
- most mice lack inguinal, iliac, sacral, brachial, and axillary lymph nodes
- a full set of mesenteric lymph nodes were observed
- Normal - however, a full set of mesenteric lymph nodes is observed

abnormal lymph node germinal center morphology
- germinal centers form in lymph nodes after immunization with T-cell dependent antigen, however they are misplaced and smaller

small Peyer's patches
- Peyer's patches that are observed are small

abnormal B cell physiology
- impaired trafficking of B cells to lymphoid follicles

absent brachial lymph nodes
- in most mice

abnormal Peyer's patch morphology
- Peyer's patches that are observed lack the characteristic multi-domed structure

absent inguinal lymph nodes
- in most mice

decreased Peyer's patch number

hematopoietic system phenotype

abnormal spleen marginal zone morphology
- outer marginal zone
- spleen shows a thickened ring of IgM^hiIgD^lo marginal zone B cells and the boundary between the B cells in this area and the inner ring of B cells is disrupted, with increased numbers of IgM^hiIgD^lo cells in the outer marginal zone

decreased spleen germinal center size
- germinal centers form in spleen after immunization with T-cell dependent antigen, however they are misplaced and smaller

abnormal spleen B cell follicle morphology
- boundary between B-cell rich areas and T zones are often poorly demarcated, with increased numbers of B cells and T cells in reciprocal areas
- B cells fail to organize into polarized follicular clusters and appear as a ring of cells at the perimeter of T-cell areas

abnormal B cell physiology
- impaired trafficking of B cells to lymphoid follicles

The following phenotype relates to a compound genotype created using this strain

Genotype: Cxcl13^tm1Cys/Cxcl13^tm1Cys
B6.129X1-Cxcl13

hematopoietic system phenotype

abnormal B cell physiology
- germinal center (GC) B cells migrate more slowly in culture than wild-type GC B cells; cells show a slower velocity and less mean displacement over time compared to wild-type cells

immune system phenotype

abnormal B cell physiology
- germinal center (GC) B cells migrate more slowly in culture than wild-type GC B cells; cells show a slower velocity and less mean displacement over time compared to wild-type cells

References

Ansel KM; Ngo VN; Hyman PL; Luther SA; Forster R; Sedgwick JD; Browning JL; Lipp M; Cyster JG. 2000. A chemokine-driven positive feedback loop organizes lymphoid follicles. Nature 406(6793):309-14PubMed: 10917533 MGI: J:78282

Additional - Cxcl13^tm1Cys related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Cxcl13
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice are maintained as homozygotes. Despite defective B cell function and lymph node development, the mutants live and breed normally in SPF vivaria.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the BLC- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005626 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Heterozygous or wildtype for Cxcl13<tm1Cys>

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:BLC-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Cxcl13tm1Cys

Allele Symbol: Cxcl13tm1Cys

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Cxcl13tm1Cys related

Mammalian Phenotype Terms by Genotype

Genotype: Cxcl13tm1Cys/Cxcl13tm1Cysinvolves: 129X1/SvJ * C57BL/6

immune system phenotype

hematopoietic system phenotype

Genotype: Cxcl13tm1Cys/Cxcl13tm1CysB6.129X1-Cxcl13

hematopoietic system phenotype

immune system phenotype

References

Additional - Cxcl13tm1Cys related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Cxcl13^tm1Cys

Allele Symbol: Cxcl13^tm1Cys

Genotype: Cxcl13^tm1Cys related

Genotype: Cxcl13^tm1Cys/Cxcl13^tm1Cys
involves: 129X1/SvJ * C57BL/6

Genotype: Cxcl13^tm1Cys/Cxcl13^tm1Cys
B6.129X1-Cxcl13

Additional - Cxcl13^tm1Cys related