These Cxcl13 knock-out mice exhibit defective B cell organization, and are suitable for use in applications related to lymph node and Peyer's patch development.
Jason Cyster, University of California San Francisco
Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. No endogenous gene product (mRNA or protein) is detected in spleen or mesenteric lymph node. EGFP is not expressed. Severe, but incompletely penetrant, defects of the inguinal, iliac, axillary, brachial, popliteal, deep cervical, renal, sacral, and parathymic lymph nodes are observed. The lymphoid patch of the cecum is absent and Peyer's patches are severely reduced and typically malformed. This mutant has defective B cell organization, an absence of primary follicle follicular dendritic cells, and reduced B cell LTa1b2 expression in spleen and lymph nodes. This mutant may be useful in B cell, follicular dendritic cell, and/or lymph node development studies.
A targeting vector was constructed in which base pairs 18-116 of exon 2 from the endogenous gene were replaced with an in-frame stop codon, a Mengo virus internal ribosome entry site, an enhanced green fluorescent protein gene (EGFP), and a loxP-flanked neomycin resistance gene. The construct was electroporated into 129X1/SvJ derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were backcrossed for germ-line transmission to C57BL/6J females. Offspring were mated with Cre-expressing C57BL/6J to remove the neomycin resistance gene. The neo-excised heterozygotes were backcrossed to C57BL/6J for ten generations before being made homozygous.
|Allele Name||targeted mutation 1, Jason G Cyster|
|Allele Type||Targeted (Reporter, Null/Knockout)|
|Allele Synonym(s)||BLC-; CXCL13-|
|Gene Symbol and Name||Cxcl13, chemokine (C-X-C motif) ligand 13|
|Strain of Origin||129X1/SvJ|
|Molecular Note||Base pairs 18 through 116 (exon 2) were replaced by a cassette containing a stop codon followed by an internal ribosome entry site, an enhanced green fluorescent protein gene, and a floxed neo gene. The deleted region encoded amino acids 27 through 60, including 3 of the 4 conserved Cys residues. The absence of transcript and protein in homozygous mutant mice was confirmed by Northern and Western blot analyses of homozygous mutant mice.|
|Mutations Made By|| |
Jason Cyster, University of California San Francisco
When maintaining a live colony, these mice are maintained as homozygotes. Despite defective B cell function and lymph node development, the mutants live and breed normally in SPF vivaria.
When using the BLC- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005626 in your Materials and Methods section.
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