Tamoxifen administration in these mice induces Cre recombinase expression in all cells of the mouse limb that express the endogenous gene. Cells that contribute to more anterior digits cease to express Shh at an earlier stage of limb development.This mutant mouse strain may be useful in studies of limb patterning and development.
Clifford J Tabin, Harvard Medical School
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing, Inducible) | Shh | sonic hedgehog |
This strain expresses a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain from the endogenous Shh locus. The mutant human estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces Cre recombinase expression in all cells of the mouse limb that express the endogenous gene resulting in the deletion of the first 35 base pairs following the ATG. Introduction of tamoxifen at E10.5 resulted in the labeling of LacZ-positive cells that contributed to digit 5 and the posterior half of digit 4 in E14.5 limbs. Injection of tamoxifen at E11.5 resulted in the labeling of cells only in digit 5. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of limb patterning and development.
A targeting vector containing a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain was inserted at the ATG of Shh. The construct was electroporated into 129S6/SvEv-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reported that the resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for five generations (see SNP note below).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
In 2018, genotyping results suggest that the Shhtm2(cre/ERT2)Cjt allele contains a neomycin resistance gene.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
---|---|
Site of Expression | tamoxifen inducible Cre recombinase protein fused to a mutant form of the human estrogen receptor ligand binding domain; expression in all cells that express the Shh gene |
Allele Name | targeted mutation 2, Clifford J Tabin |
---|---|
Allele Type | Targeted (Recombinase-expressing, Inducible) |
Allele Synonym(s) | ShhCreER; ShhCreERT2; ShhCreesr; Shhtm2(cre/ESR1)Cjt; Shhtm2(cre-ERT2)Cjt; Shh-cre; ShhCreER; ShhcreERT2 |
Gene Symbol and Name | Shh, sonic hedgehog |
Gene Synonym(s) | |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | tamoxifen inducible Cre recombinase protein fused to a mutant form of the human estrogen receptor ligand binding domain; expression in all cells that express the Shh gene |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 5 |
Molecular Note | A cassette containing CreERT2 was inserted at the ATG. Upon recombination, the first 35 base pairs after the ATG were deleted. Cre was produced in all cells in which the endogenous mRNA was normally expressed. |
Mutations Made By | Clifford Tabin, Harvard Medical School |
When maintaining a live colony, heterozygous mice are bred to wildtype siblings (or C57BL/6J). Homozygous mice are not viable.
When using the ShhcreERT2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005623 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Shh<tm2(cre/ESR1)Cjt> |
Frozen Mouse Embryo | B6.129S6-Shh<tm2(cre/ERT2)Cjt>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Shh<tm2(cre/ERT2)Cjt>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Shh<tm2(cre/ERT2)Cjt>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Shh<tm2(cre/ERT2)Cjt>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.