Mice homozygous for this spontaneous recessive mutation are recognized by a diluted coat color that appears chocolate brown. This color dilution also lightens the eyes, feet, ears, and tail of affected animals.

Development

The chtl^2J re-mutation of chtl arose spontaneously on the B6.V-Lep^ob/J strain at The Jackson Laboratory in 1997 when that strain was at N24F1. A chtl^2J homozygous female from this intercross was bred with an unaffected sibling and homozygotes were produced. This strain has been maintained by sibling breeding homozygotes with heterozygotes, of either gender, and in 2005 reached generation F25. Obese mice (Lep^ob homozygotes) have never been found in this chocolate like 2 Jackson subline.

Control Suggestions

Heterozygote from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Samples RM; Ward-Bailey PF; Donahue LR; Bronson RT; Davisson MT. 2005. Chocolate-like 2 Jackson, a remutation of the chtl gene MGI Direct Data SubmissionMGI: J:98900

View All References

Genetics

chtl^2J

Allele Symbol: chtl^2J

Allele Name	chocolate-like 2 Jackson
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	chtl, chocolate-like
Gene Synonym(s)
Strain of Origin	B6.Cg-Lep^ob/J
Chromosome	7
General Note	Tests for allelism to cht and chtl were considered inconclusive because the F2s produced in both tests resulted in two coat colors, and while indicating some interaction between the new mutation and the chocolate mutation, does not prove that the new mutation is a remutation to chocolate.
Molecular Note	This spontaneous mutation occurred at The Jackson Laboratory.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: chtl^2J related

Dermatology Research
- Color and White Spotting Defects
Cell Biology Research
- Vesicular Trafficking

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: chtl^2J/chtl^2J
B6(V)-chtl<2J>/GrsrJ

craniofacial phenotype

decreased ear pigmentation

vision/eye phenotype

decreased eye pigmentation
- this mutation lightens the eye color

growth/size/body region phenotype

decreased ear pigmentation

hearing/vestibular/ear phenotype

decreased ear pigmentation

integument phenotype

abnormal skin pigmentation
- the feet, ears and tail appear lighter

decreased ear pigmentation

decreased tail pigmentation

diluted coat color
- the coat color on a C57BL/6J background appears dark chocolate brown

limbs/digits/tail phenotype

decreased tail pigmentation

pigmentation phenotype

abnormal skin pigmentation
- the feet, ears and tail appear lighter

decreased ear pigmentation

decreased eye pigmentation
- this mutation lightens the eye color

decreased tail pigmentation

diluted coat color
- the coat color on a C57BL/6J background appears dark chocolate brown

References

Samples RM; Ward-Bailey PF; Donahue LR; Bronson RT; Davisson MT. 2005. Chocolate-like 2 Jackson, a remutation of the chtl gene MGI Direct Data SubmissionMGI: J:98900

Additional - chtl^2J related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the Chocolate-like(2J) mouse strain in a publication, please cite the originating article(s) and include JAX stock #005565 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or Homozygous for chtl<2J>, 1 pair minimum

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

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Also Known As:Chocolate-like(2J)

Genetic overview

Research Applications

Base Price

Detailed Description