These Line B mice carry a transgene expressing a mutated insulin, and approximately 75% of females become diabetic.
George Eisenbarth, U of Colorado
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Expression has been reported in the pancreatic islets and thymus of NOD mice carrying the Tg(Ins2*Y16A)1Ell mutation. Approximately 75% of line B female transgenic mice become diabetic in the presence of native insulin genes by 35 weeks of age. NOD female transgenic mice lacking both Ins1 and Ins2 fail to produce insulin autoantibodies, and there is no diabetes or insulitis at 26 weeks of age, but sialitis is present. Donating investigator reports male transgenics lacking both Ins1 and Ins2 develop metabolic diabetes before 10 weeks of age. In contrast, transgenic female mice in the presence of Ins1 and lacking Ins2 develop diabetes in 75% of the animals by 25 weeks of age (Nakayama et al, 2004, 2005). Donating investigator reports line B transgenics have lower expression levels than line F and that male transgenics lacking both Ins1 and Ins2 develop metabolic diabetes before 10 weeks of age.
NOD-Tg(Ins2*Y16A)1Ell/GseJ is useful to study insulin-reactive autoimmunity.
NOD-Tg(Ins2*Y16A)1Ell/GseJ expresses the rat insulin 7 promoter fused to a mutated preproinsulin II cDNA, where the tyrosine at position 16 of the b chain was replaced with alanine. This transgene was inserted by Nakayama et al, 2004 directly into NOD/MrkTac oocytes. Founder line B has been maintained on the NOD background. In 2005, The Jackson Laboratory received NOD-Tg(Ins2*Y16A)1Ell/GseJ at generation N3.
Expressed Gene | Ins2, insulin 2, rat |
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Site of Expression |
Allele Name | transgene insertion 1, John F Elliot |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | tg B:16 alanine, line B |
Gene Symbol and Name | Tg(Ins2*Y16A)1Ell, transgene insertion 1, John F Elliot |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | Ins2, insulin 2, rat |
Strain of Origin | NOD/MrkTac |
Chromosome | UN |
Molecular Note | This transgenic construct contains the rat insulin7 construct of the rat insulin 2 gene fused to a mutated preproinsulin II cDNA where the tyrosine at position 16 of the B chain was replaced with alanine. The transgene is expressed in pancreatic beta cells. |
Mutations Made By | John Elliott, University of Alberta |
When using the NOD.Tg B:16 alanine, line B (low) mouse strain in a publication, please cite the originating article(s) and include JAX stock #005522 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Ins2*Y16A)1Ell |
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