These transgenic mice express the human MHC class I HLA-A2.1 molecule.
Dr. David Serreze, The Jackson Laboratory
Genetic Background | Generation |
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001289 NOD/ShiLt |
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Allele Type |
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Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
Homozygous mice carrying the (HLA-A2.1)1Enge transgene ubiquitously express significant quantities of the human MHC class I HLA-A2.1 molecules. These HLA-A2.1 molecules mediate beta cell auto-reactive CD8 T cell responses that when added to those elicited by endogenous murine MHC class I variants results in a significantly accelerated rate of diabetes onset in either the hemizygote or homozygote transgenic mouse when compared to NOD/Lt controls (Marron et al., 2002). The transgene integrated into chromosome 8 causing a duplication in Mcph1 (microcephaly, primary autosomal recessive 1). The duplication results in a functional knock-out of Mcph1 in homozygous mice.
This model is useful for studying the role of MHC class I molecules in Type 1 Diabetes.
Using marker assisted analysis for all known Idd loci, the transgenic allele HLA-A2.1 from C57BL/6-Tg(HLA-A2.1)1Enge/J (stock# 3088) was backcrossed 10 generations to NOD/Lt prior to making homozygote (Marron et al., 2002). In 2005, the Type 1 Diabetes Resource received NOD.B6-Tg(HLA-A2.1)1Enge/Dvs at N10F34. The Tg(HLA-A2.1)1Enge transgene integrated into chromosome 8 causing a duplication in Mcph1 (microcephaly, primary autosomal recessive 1).
Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human |
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Site of Expression |
Allele Name | transgene insertion 1, Victor H Engelhard |
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Allele Type | Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Mcph1, microcephaly, primary autosomal recessive 1 |
Gene Synonym(s) | |
Promoter | HLA-A2.1, major histocompatibility complex, class I, subtype A2.1, human |
Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human |
Strain of Origin | C57BL/6 |
Chromosome | 8 |
General Note | Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. |
Molecular Note | The transgene contains the full length HLA-A2.1 gene. Expression of human class I MHC Ag HLA-A2.1 was detected on cells from homozygous transgenic spleen, bone marrow, and thymus. Line 1 inserted into the gene at 18736683-18757058 (Build GRCm38/mm10) resulting in a duplication. The duplication results in a functional knock-out. Founder line 1 has a copy number of 3-8. |
Mutations Made By | Dr. Ai-Xuan Le, Stanford University |
A footpad injection of Complete Freund s Adjuvant (CFA) administered once at weaning will delay diabetes onset, thus extending the lifespan of breeders. Use of Complete Freund s Adjuvant in NOD mice can be found in Current Protocols in Immunology page 15.9.19, Reproduction.
When using the NOD.HLA-A2.1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005512 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Tg(HLA-A2.1)1Enge, 1 pair minimum |
Frozen Mouse Embryo | NOD.B6-Mcph1<Tg(HLA-A2.1)1Enge>/DvsJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.B6-Mcph1<Tg(HLA-A2.1)1Enge>/DvsJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.B6-Mcph1<Tg(HLA-A2.1)1Enge>/DvsJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | NOD.B6-Mcph1<Tg(HLA-A2.1)1Enge>/DvsJ Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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