STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J

Stock No:: 005493 |; Tie2-rTA/TRE-βGal

Also Known As: Tie2-rTA/TRE-βGal

These transgenic mice harbor the reverse tetracycline-controlled transactivator (rtTA) and the beta-galactosidase gene (lacZ), and in the presence of tetracycline or a tetracycline analog, expression of lacZ is observed in the nuclei of vascular endothelium in a wide variety of tissues.

Donating Investigator

James N Topper, Frazier Healthcare Ventures

Genetic overview

Genetic Background	Generation

Tg(Tek-rtTA,TRE-lacZ)1425Tpr

Allele Type
Transgenic (Inducible, Reporter)

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Research Applications

Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice harbor co-injected transgenic constructs. The reverse tetracycline-controlled transactivator (rtTA) protein is expressed under the direction of the endothelial-specific receptor tyrosine kinase enhancer/promoter (Tek). The second transgenic construct expresses a nuclear-localizing beta-galactosidase gene (lacZ) under the control of a tetracycline-responsive element (TRE). In the presence of tetracycline or a tetracycline analog (such as doxycycline), selective expression of lacZ is observed in the nuclei of vascular endothelium in a wide variety of tissues (aorta, heart, brain, lung, kidney, liver, spleen, uterus, prostate, stomach, skeletal muscle, large and small intestine). Expression is observed as early as embryonic day 9.5. In the absence of tetracycline, some lacZ expression occurs in the smaller branches of the aorta. Additionally, the donating investigators report a trend towards diminished lacZ expression when the alleles are crossed onto a C57BL/6 background. This strain provides a tool that allows the inducible expression of genes in the vascular endothelium during early developmental stages and in the adult.

This strain is on a genetic background different from that on which the allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

To generate this transgenic line, two transgenic constructs were co-injected into B6CBA fertilized oocytes. The first construct included a 2.1 kb endothelial-specific receptor tyrosine kinase (Tek) promoter fragment, the Tet-on transactivator (tTA), SV40 polyadenylation sequence and a 1.7 kb enhancer sequence derived from Tek intron 1. The other construct consisted of a tetracycline-responsive element, nuclear-localizing beta-galactosidase (lacZ) gene and a SV40 polyadenylation sequence. Although the transgenic constructs are closely linked in this transgenic line (1425), the donating investigators report that no definitive evidence exits indicating that they have cointegrated. The mice were later crossed to Apoe mutant mice (that may have been congenic on the C57BL/6 background). During the development of the colony at The Jackson Laboratory, mice were selected for the (Tek-rtTA,TRE-lacZ) transgene and against the Apoe mutant allele.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Expressed Gene	rtTA, reverse tetracycline-controlled transactivator, E. coli
Site of Expression	Expresses rtTA in vascular endothelial cells.

Control Suggestions

Noncarrier ()

Additional Information

Considerations for Choosing Controls

Selected References

Teng PI; Dichiara MR; Komuves LG; Abe K; Quertermous T; Topper JN. 2002. Inducible and selective transgene expression in murine vascular endothelium. (2): 99-107. PubMed: 12388791 MGI: 84020

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Genetics

Tg(Tek-rtTA,TRE-lacZ)1425Tpr

Allele Symbol: Tg(Tek-rtTA,TRE-lacZ)1425Tpr

Allele Name	transgene insertion 1425, James N Topper
Allele Type	Transgenic (Inducible, Reporter)
Allele Synonym(s)
Gene Symbol and Name	Tg(Tek-rtTA,TRE-lacZ)1425Tpr, transgene insertion 1425, James N Topper
Gene Synonym(s)
Promoter	tetO, tet operator,
Promoter	Tek, TEK receptor tyrosine kinase, mouse, laboratory
Expressed Gene	lacZ, beta-galactosidase, E. coli
Expressed Gene	rtTA, reverse tetracycline-controlled transactivator, E. coli
Site of Expression	Expresses rtTA in vascular endothelial cells.
Strain of Origin	(C57BL/6 x CBA)F1
Chromosome	UN
General Note	Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In the presence of tetracycline or a tetracycline analog such as doxycycline, selective expression of beta-galactosidase is observed in the nuclei of vascular endothelium in a wide variety of tissues: aorta, heart, brain, lung, kidney, liver, spleen, uterus, prostate, stomach, skeletal muscle, large and small intestine. Expression is observed as early as embryonic day 9.5. In the absence of tetracycline, some beta-galactosidase expression occurs in the smaller branches of the aorta. A trend towards diminished beta-galactosidase expression when the alleles are crossed onto a C57BL/6 background has been reported.
Molecular Note	Two transgenic constructs were co-injected into B6CBA fertilized oocytes. The first construct included a 2.1 kb endothelial-specific receptor tyrosine kinase (Tek) promoter fragment, the Tet-on transactivator, SV40 polyadenylation sequence and a 1.7 kb enhancer sequence derived from Tek intron 1. The other construct consisted of a tetracycline-responsive element, nuclear-localizing beta-galactosidase gene and a SV40 polyadenylation sequence. Although the transgenic constructs are closely linked in transgenic line 1425, no definitive evidence exits indicating that they have cointegrated.
Mutations Made By	Peter Teng, Millennium Pharmaceuticals

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Tet Expression Systems
  - tTA/rtTA Responsive Strains
  - tTA/rtTA Expressing Strains
- Cardiovascular Research
  - Tetop Tet System
  - endothelial cell marker (lacZ) for neovascularization
  - endothelial cell marker for neovascularization
- Genetics Research
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers: endothelial cells
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis: Tetop Tet System
  - Tissue/Cell Markers: endothelial cell markers for neovascularization
- lacZ Expression
- Cancer Research
  - Tetop Tet System
  - endothelial cell marker for neovascularization

Mammalian Phenotype Terms by Genotype

References

Teng PI; Dichiara MR; Komuves LG; Abe K; Quertermous T; Topper JN. 2002. Inducible and selective transgene expression in murine vascular endothelium. (2): 99-107. PubMed: 12388791 MGI: 84020

Additional - Tg(Tek-rtTA,TRE-lacZ)1425Tpr

Kirov A; Duarte M; Guay J; Karolak M; Yan C; Oxburgh L; Prudovsky I. 2012. Transgenic expression of nonclassically secreted FGF suppresses kidney repair. (5): e36485. PubMed: 22606265 MGI: 188340
Sawada N; Jiang A; Takizawa F; Safdar A; Manika A; Tesmenitsky Y; Kang KT; Bischoff J; Kalwa H; Sartoretto JL; Kamei Y; Benjamin LE; Watada H; Ogawa Y; Higashikuni Y; Kessinger CW; Jaffer FA; Michel T; Sata M; Croce K; Tanaka R; Arany Z. 2014. Endothelial PGC-1alpha mediates vascular dysfunction in diabetes. (2): 246-58. PubMed: 24506866 MGI: 211750

Technical Support

Contact Technical Support

Genotyping Protocols
- Probe: Generic tTA/rtTA
- Standard PCR: Tg(tTA)
- Standard PCR: Generic LacZ
- MELT: Generic LacZ Melt Curve Analysis
- MELT: Tg(tTA)
- Genotyping resources and troubleshooting

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
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Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

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We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Also Known As: Tie2-rTA/TRE-βGal

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Tek-rtTA,TRE-lacZ)1425Tpr

Allele Symbol: Tg(Tek-rtTA,TRE-lacZ)1425Tpr

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tg(Tek-rtTA,TRE-lacZ)1425Tpr

Genotyping Protocols

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability