These mice carry an ENU-induced mutation characterized by body tremors and hind limb spasms.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.Read More +
The mutants show intense body tremor at rest and during movement, and brief, intermittent hind limb spasms that may occur uni-or bilaterally; at times they use both hind limbs simultaneously to move forward. On-set of the phenotype is at approximately 5 weeks of age (average 4.8+/- 1.4 weeks; n=131), but increases in severity with age.
Complementation analysis showed NMF379 to be an allele of Sptbn4 (Stock No. 002433, C3H/HeJ-Sptbn4qv-lnd2J/J), i.e. heterozygote matings produced 4 affected mice in a total of 10 progeny. Standard pathology work-up on two mutants (78 or 128 days of age) revealed no abnormalities A colony needs to be maintained through ovarian transplants.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||quivering 8 Jackson|
|Allele Type||Chemically induced (ENU)|
|Allele Synonym(s)||neuroscience mutagenesis facility, 379; NMF379|
|Gene Symbol and Name||Sptbn4, spectrin beta, non-erythrocytic 4|
|Strain of Origin||C57BL/6J|
|Molecular Note||This phenotypic mutation was identified in an ENU mutagenesis screen.|
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