This BALB congenic strain carries the T lymphocyte specific Thy1a (Thy1.1) allele. Donor T cells can be distinguished from recipient T cells by both flow cytometry and histological analysis.
This strain originally was developed from B6.PL-Thy1a/CyJ. The a allele was backcrossed to BALB/cJ at Scripps for approximately 16 generations. It was then crossed with a BALB transgenic to create C.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ (Stock #5307). At some point, the BALB/cByJ background was introduced into the transgenic strain. The Jackson Laboratory imported the transgenic in 2005. A genome scan performed at The Jackson Laboratory in 2005 confirmed that the strain background is BALB/cByJ. The Thy1a allele was separated from the transgenic strain to establish this congenic.
|Allele Name||a variant|
|Allele Type||Not Applicable|
|Allele Synonym(s)||thetaAKR; theta-AKR; Thy1.1; Thy-1.1; Thy1a|
|Gene Symbol and Name||Thy1, thymus cell antigen 1, theta|
|Site of Expression||The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.|
|Strain of Origin||Not Applicable|
|General Note|| |
The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).
|Molecular Note||The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains.|
When using the CBy.PL(B6)-Thy1a/ScrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #005443 in your Materials and Methods section.