Overview

Also Known As:RIP-OVA^lo

This strain is currently unavailable due to replenishing of cryopreserved stocks. Interested customers who register interest will be contacted when the strain is again available.

These RIP-OVA^lo mice express a transgene containing a secreted form of ovabumin and exhibit no detectable ovalbumin in pancreatic islets. This ovalbumin expressing model is a tool for assessing antigen ignorance and is used in tumor immunity studies and tissue damage studies.

Donating Investigator

William Heath, The Walter and Eliza Hall Institute

Genetic overview

Genetic Background	Generation

Tg(Ins2-OVA)307Wehi

Allele Type
Transgenic (Inserted expressed sequence)

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Research Applications

Research Tools
Immunology, Inflammation and Autoimmunity Research

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Details

Detailed Description

Immunohistochemistry does not detect ovalbumin in the islet beta cells of the pancreas. The double transgenic resulting from a cross of this transgenic stock with C57BL/6-Tg(Tcra Tcrb)1100MjbJ (OT-1) exhibit early onset of spontaneous diabetes with islet infiltration; implying that the beta cells express OVA. One of 60 irradiated Tg(Ins2-OV)307 mice receiving a 1:4 mixture (OT-1 transgenic mice:C57BL/6-Thy1.1 congenic) of bone marrow become diabetic when followed for 10 weeks post transfer. Histolocigal evaluation indicates 22% of the islets are mildly infiltrated 15-21 weeks post transfer. This stock only exhibits antigen presentation in the draining lymph node when the pancreatic islets have been damaged. Hemizygotes are viable and fertile.

This ovalbumin expressing model is a tool for assessing antigen ignorance and is used in tumor immunity studies and tissue damage studies.

Development

C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA (OV) under the control of the rat insulin 2 promoter (Ins2). This transgene was injected into congenic strain C57BL/6-H-2^bm1 oocytes. Resulting progeny from founder 307-3 were backcrossed to C57BL/6 for 5 generations prior to intercrossing. The Jackson Laboratory received C57BL/6-Tg(Ins2-OV)307Wehi/WehiJ at generation N7.

Expression Data

Expressed Gene	OVA, ovalbumin, chicken
Site of Expression

Control Suggestions

Noncarrier
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Blanas E; Carbone FR; Allison J; Miller JF; Heath WR. 1996. Induction of autoimmune diabetes by oral administration of autoantigen. Science 274(5293):1707-9PubMed: 8939860 MGI: J:99451

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Genetics

Tg(Ins2-OVA)307Wehi

Allele Symbol: Tg(Ins2-OVA)307Wehi

Allele Name	transgene insertion 307, Walter and Eliza Hall Institute of Medical Research
Allele Type	Transgenic (Inserted expressed sequence)
Allele Synonym(s)	RIP-OVA; RIP-OVA^lo
Gene Symbol and Name	Tg(Ins2-OVA)307Wehi, transgene insertion 307, Walter and Eliza Hall Institute of Medical Research
Gene Synonym(s)
Promoter	Ins2, insulin 2, rat
Expressed Gene	OVA, ovalbumin, chicken
Strain of Origin	B6.C-H2^bm1/ByJWehi
Chromosome	UN
General Note	Immunohistochemical analysis does not detect ovalbumin (OV) in the pancreatic islet beta cells of mice bearing this transgene, nor does western blot analysis, indicating that their OV content is less than 0.03 ng/ug of protein. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an ovalbumin-specific T cell receptor expressed on CD8+ T lymphocytes, develop early onset, spontaneous diabetes with islet infiltration, implying that the beta cells express OV. While only one of 60 lethally irradiated Tg(Ins2-OV)307Wehi mice receiving a 1:4 (OT-1 mouse:C57BL/6-Thy1^a congenic) bone marrow mixture became diabetic during the 10 weeks post transfer, histological evaluation of these mice at 15-21 weeks post-transfer showed mild infiltration of 22% of their islets.
Molecular Note	This transgene expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA under control of the rat insulin 2 promoter.
Mutations Made By	William Heath, The Walter and Eliza Hall Institute

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T Cell Receptor Transgenics
- Diabetes and Obesity Research
Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
  - Type 1 Diabetes
- CD Antigens, Antigen Receptors, and Histocompatibility Markers

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tg(Ins2-OVA)307Wehi/?
involves: BALB/c * C57BL/6

endocrine/exocrine gland phenotype

abnormal pancreatic beta cell physiology
- secretion is inferred by the infiltration of ovalbumin specific T cells
- beta cells secrete very low amounts of a soluble form of chicken ovalbumin (OVA)

immune system phenotype

increased susceptibility to autoimmune diabetes
- mice die from diabetes within 12 days of transfer of OVA-specific CD8 T cells

References

Blanas E; Carbone FR; Allison J; Miller JF; Heath WR. 1996. Induction of autoimmune diabetes by oral administration of autoantigen. Science 274(5293):1707-9PubMed: 8939860 MGI: J:99451

Additional - Tg(Ins2-OVA)307Wehi related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tg(Ins2-OVA)
- Standard PCR:Tg(Ins2-OVA)
- Standard PCR:Tg(Ins2-OVA)
- Standard PCR:Tg(Ins2-OVA)
- Standard PCR:Tg(Ins2-OVA)
- Standard PCR:Tg(Ins2-OVA)
- QPCR:Tg(Ins2-OVA)
- QPCR:Tg(Ins2-OVA)
- Standard PCR:Tg(Ins2-TFRC/OVA)296Wehi Alternate3
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, hemizygous mice may be bred to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygous viability/fertility has not been tested.
- Additional Breeding and Husbandry Support
Appearance
- black
  Related Genotype: a/a
Citation
When using the RIP-OVA^lo mouse strain in a publication, please cite the originating article(s) and include JAX stock #005432 in your Materials and Methods section.

Strain Interest Registration

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This information helps us manage the colony build and better meet the broad needs of the research community.
Please send any technical questions to Technical Support.

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Terms Of Use

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Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:RIP-OVAlo

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Ins2-OVA)307Wehi

Allele Symbol: Tg(Ins2-OVA)307Wehi

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tg(Ins2-OVA)307Wehi/?involves: BALB/c * C57BL/6

endocrine/exocrine gland phenotype

immune system phenotype

References

Additional - Tg(Ins2-OVA)307Wehi related

Genotyping Protocols

Breeding Considerations

Appearance

Citation

Strain Interest Registration

Contact Information

Interest

Terms Of Use

Licensing Information

Also Known As:RIP-OVA^lo

Genotype: Tg(Ins2-OVA)307Wehi/?
involves: BALB/c * C57BL/6