C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ

Stock number: 005432
Product name: RIP-OVA^lo
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These RIP-OVA^lo mice express a transgene containing a secreted form of ovabumin and exhibit no detectable ovalbumin in pancreatic islets. This ovalbumin expressing model is a tool for assessing antigen ignorance and is used in tumor immunity studies and tissue damage studies.

Detailed description

Immunohistochemistry does not detect ovalbumin in the islet beta cells of the pancreas. The double transgenic resulting from a cross of this transgenic stock with C57BL/6-Tg(Tcra Tcrb)1100MjbJ (OT-1) exhibit early onset of spontaneous diabetes with islet infiltration; implying that the beta cells express OVA. One of 60 irradiated Tg(Ins2-OV)307 mice receiving a 1:4 mixture (OT-1 transgenic mice:C57BL/6-Thy1.1 congenic) of bone marrow become diabetic when followed for 10 weeks post transfer. Histolocigal evaluation indicates 22% of the islets are mildly infiltrated 15-21 weeks post transfer. This stock only exhibits antigen presentation in the draining lymph node when the pancreatic islets have been damaged. Hemizygotes are viable and fertile.

This ovalbumin expressing model is a tool for assessing antigen ignorance and is used in tumor immunity studies and tissue damage studies.

Development

C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA (OV) under the control of the rat insulin 2 promoter (Ins2). This transgene was injected into congenic strain C57BL/6-H-2^bm1 oocytes. Resulting progeny from founder 307-3 were backcrossed to C57BL/6 for 5 generations prior to intercrossing. The Jackson Laboratory received C57BL/6-Tg(Ins2-OV)307Wehi/WehiJ at generation N7.

Allele

Symbol: Tg(Ins2-OVA)307Wehi
Name: transgene insertion 307, Walter and Eliza Hall Institute of Medical Research
MGI accession ID: MGI:3583231
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Ins2-OVA)307Wehi
Marker name: transgene insertion 307, Walter and Eliza Hall Institute of Medical Research
Chromosome: UN
Strain of origin: B6.C-H2^bm1/ByJWehi
Expressed genes: OVA,ovalbumin,chicken
Molecular note: This transgene expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA under control of the rat insulin 2 promoter.
General note: Immunohistochemical analysis does not detect ovalbumin (OV) in the pancreatic islet beta cells of mice bearing this transgene, nor does western blot analysis, indicating that their OV content is less than 0.03 ng/ug of protein. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an ovalbumin-specific T cell receptor expressed on CD8+ T lymphocytes, develop early onset, spontaneous diabetes with islet infiltration, implying that the beta cells express OV. While only one of 60 lethally irradiated Tg(Ins2-OV)307Wehi mice receiving a 1:4 (OT-1 mouse:C57BL/6-Thy1^a congenic) bone marrow mixture became diabetic during the 10 weeks post transfer, histological evaluation of these mice at 15-21 weeks post-transfer showed mild infiltration of 22% of their islets.