These RIP-OVAlo mice express a transgene containing a secreted form of ovabumin and exhibit no detectable ovalbumin in pancreatic islets. This ovalbumin expressing model is a tool for assessing antigen ignorance and is used in tumor immunity studies and tissue damage studies.
William Heath, The Walter and Eliza Hall Institute
Genetic Background | Generation |
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|
Allele Type |
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Transgenic (Inserted expressed sequence) |
Immunohistochemistry does not detect ovalbumin in the islet beta cells of the pancreas. The double transgenic resulting from a cross of this transgenic stock with C57BL/6-Tg(Tcra Tcrb)1100MjbJ (OT-1) exhibit early onset of spontaneous diabetes with islet infiltration; implying that the beta cells express OVA. One of 60 irradiated Tg(Ins2-OV)307 mice receiving a 1:4 mixture (OT-1 transgenic mice:C57BL/6-Thy1.1 congenic) of bone marrow become diabetic when followed for 10 weeks post transfer. Histolocigal evaluation indicates 22% of the islets are mildly infiltrated 15-21 weeks post transfer. This stock only exhibits antigen presentation in the draining lymph node when the pancreatic islets have been damaged. Hemizygotes are viable and fertile.
This ovalbumin expressing model is a tool for assessing antigen ignorance and is used in tumor immunity studies and tissue damage studies.
C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA (OV) under the control of the rat insulin 2 promoter (Ins2). This transgene was injected into congenic strain C57BL/6-H-2bm1 oocytes. Resulting progeny from founder 307-3 were backcrossed to C57BL/6 for 5 generations prior to intercrossing. The Jackson Laboratory received C57BL/6-Tg(Ins2-OV)307Wehi/WehiJ at generation N7.
Expressed Gene | OVA, ovalbumin, chicken |
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Site of Expression |
Allele Name | transgene insertion 307, Walter and Eliza Hall Institute of Medical Research |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | RIP-OVA; RIP-OVAlo |
Gene Symbol and Name | Tg(Ins2-OVA)307Wehi, transgene insertion 307, Walter and Eliza Hall Institute of Medical Research |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | OVA, ovalbumin, chicken |
Strain of Origin | B6.C-H2bm1/ByJWehi |
Chromosome | UN |
General Note | Immunohistochemical analysis does not detect ovalbumin (OV) in the pancreatic islet beta cells of mice bearing this transgene, nor does western blot analysis, indicating that their OV content is less than 0.03 ng/ug of protein. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an ovalbumin-specific T cell receptor expressed on CD8+ T lymphocytes, develop early onset, spontaneous diabetes with islet infiltration, implying that the beta cells express OV. While only one of 60 lethally irradiated Tg(Ins2-OV)307Wehi mice receiving a 1:4 (OT-1 mouse:C57BL/6-Thy1a congenic) bone marrow mixture became diabetic during the 10 weeks post transfer, histological evaluation of these mice at 15-21 weeks post-transfer showed mild infiltration of 22% of their islets. |
Molecular Note | This transgene expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA under control of the rat insulin 2 promoter. |
Mutations Made By | William Heath, The Walter and Eliza Hall Institute |
When maintaining a live colony, hemizygous mice may be bred to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygous viability/fertility has not been tested.
When using the RIP-OVAlo mouse strain in a publication, please cite the originating article(s) and include JAX stock #005432 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrierfor Tg(Ins2-OVA)307Wehi |
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