Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by RT-PCR analysis of activated spleen lymphocytes. No telomerase activity is detected by telomeric repeat amplification (TRAP) assay analysis of testis cells. Mice homozygous for the targeted mutation lack telomerase activity and exhibit telomere shortening. Homozygous intercrossing for multiple generations causes telomere instability (shortening) and infertility. This mutant mouse strain may be useful in studies of telomerase function.
A targeting vector containing Green Fluorescent Protein and neomycin resistance genes was used to disrupt exon 1. The construct was electroporated into 129S1/Sv-p+ Tyr+ KitlSl-J derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 18 generations.
|Allele Name||targeted mutation 1, Y Jeffrey Chiang|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||mTERT-; TERT-|
|Gene Symbol and Name||Tert, telomerase reverse transcriptase|
|Strain of Origin||Not Specified|
|Molecular Note||A GFPneo was inserted into the 5' UTR of exon 1. Southern blot and PCR confirmed recombination in mutant mice. RT-PCR showed a lack of transcript in mutants and a telomerase repeat amplification (TRAP) assay indicated there was no telomerase activity in testis cell extracts from mutants.|
|Mutations Made By|| |
Jeffrey Chiang, NCI/NIH
When maintaining a live colony, these mice are bred as heterozygotes. Homozygous intercrossing for multiple generations causes infertility due to telomere shortening.
When using the TERT KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005423 in your Materials and Methods section.