C;129S7 Gt(ROSA)26Sor-Bmp5^cfe-se7J/GrsrJ

Stock number: 005420
Product name: cauliflower ear 7 Jackson
Research areas: Developmental Biology Research

Description

These mice carry a mutation of the Bmp5 gene that affects the morphology of the ear.

Detailed description

Homozygotes have small, round ear pinnae with ridges along the perimeter and both ears are affected. This mutation is 100% penetrant. Unlike short ear mutations of this gene, skeletal abnormalities were not detected by X-ray for this mutant. Both males and females are fertile.

Development

The cauliflower ear mutation arose spontaneously in 2002 in the production colony of C.129S7-Gt(ROSA)26Sor/J (stock #002255) at The Jackson Laboratory. The mutant subline was maintained by sibling breeding for 8 generations, then backcrossed once to BALB/cJ, and then intercrossed. In 2007 sperm were cryopreserved from homozygous Males at generation +F8N1F2.

Allele

Symbol: Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
MGI accession ID: MGI:1890203
Generation method: Gene trapped
Attributes: Not Applicable
Synonyms: gtrosa26; R26^tg; R26R3; ROSA26; Rosa26lacZ; ROSA26-lacZ; TgR(ROSA26)26Sor
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Marker synonyms: beta geo; Gtrgeo26; Gtrosa26; R26; ROSA26; SETD5-AS1; Thumpd3as1
Chromosome: 6
Strain of origin: 129S7/SvEvBrd-Hprt1⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: All tissues of the developing embryo and in most tissues of the adult mouse.
Molecular note: Random insertion of retroviral gene trap vector ROSA, Reverse Orientation with Splice Acceptor, which contains the beta-galactosidase gene and accompanying splice acceptor and neo sequences. The construct was inserted in reverse orientation in the retroviral vector pGen-. The transgene is controlled by an unknown endogenous promoter. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult transgenic mouse.
General note: ES cell line = AB1 or AB2.1. No overt phenotypes in heterozygous or homozygous animals. The trapped gene appears to be ubiquitously expressed during embryonic development and in all hematopoietic cells.

Allele

Symbol: Bmp5^cfe-se7J
Name: cauliflower ear short ear 7 Jackson
MGI accession ID: MGI:2679550
Generation method: Spontaneous
Marker symbol: Bmp5
Marker name: bone morphogenetic protein 5
Chromosome: 9
Strain of origin: C.129S7-Gt(ROSA)26Sor/J
Molecular note: The cauliflower ear mutation was found to be allelic with the short ear mutation through a noncomplementation test.