B6.129S6-Dp(16Cbr1-Fam3b)1Rhr/J

Stock number: 005383
Product name: Ts1Rhr
Research areas: Neurobiology Research

Description

This strain is currently unavailable due to replenishing of cryopreserved stocks.  Interested customers can register interest.

These mice carry a duplication of a 3.9 Mb region on chromosome 16 and are trisomic for the mouse chromosome segment that is orthologus to the human chromosome 21 Down syndrome critical region.

Detailed description

These mice are trisomic for the mouse chromosome segment orthologus to the human chromosome 21 Down syndrome critical region (DSCR). The borders of the 3.9 Mb region are defined by the carbonyl reductase 1 gene and a site adjacent to the myxovirus (influenza virus) resistance 2 locus. Trisomic mice are viable, fertile and are significantly larger than euploid littermates. Mandible size is enlarged as is skull size, with an overall elongation of the rostrocaudal aspect in comparison to euploid littermates. This mouse may be useful in studies exploring the consequences of polyploidy involving the DSCR.

Development

A targeting vector was used to introduce a sequence encoding a 3'portion of a neomycin resistance gene, a loxP site and a hygromycin resistance gene at the carbonyl reductase 1 locus in 129S6/SvEv-derived MC1 embryonic stem (ES) cells. Correctly targeted ES cells were selected and electroporated with another targeting vector designed to orient a puromycin resistance gene, a loxP site and a 5'portion of a neomycin resistance gene, adjacent to the myxovirus (influenza virus) resistance 2 locus. A CAGGS-cre expression vector was electroporated into ES cells harboring both targeted mutations. Cre-mediated recombinants that possessed a functional neomycin resistance gene were obtained and injected into ICR blastocysts. Chimeric animals were crossed to C57BL/6 mice for at least 8 generations (4/2005).

Allele

Symbol: Dp(16Cbr1-Fam3b)1Rhr
Name: duplication, Chr 16, R H Reeves 1
MGI accession ID: MGI:3487283
Generation method: Targeted
Attributes: Inserted expressed sequence
Marker symbol: Dp(16Cbr1-Fam3b)1Rhr
Marker name: duplication, Chr 16, H R Reeves 1
Chromosome: 16
Strain of origin: 129S6/SvEvTac
Molecular note: A duplication of a 3.9 Mb region on chromosome 16 was engineered by Cre-mediated recombination between inserted loxP sites located on different chromosomes. This duplicated segment contains mouse orthologs of 33 conserved and minimally conserved genes in the human Down syndrome critical region.