RB156Bnr/Ei Dsp^rul Gulo^sfx-2J/GrsrJ

Stock number: 005354
Product name: spontaneous fracture 2 Jackson

Description

This strain has been discontinued, please refer to Stock No. 004317 for a different allele of this gene on a different genetic background or search the JAX^® Mice Database for an alternative strain.

Detailed description

The phenotype of the sfx^2J remutation is identifiable at 4-5 weeks of age when the mutants appear smaller than their control littermates and begin to hobble about their cage. Between 5 and 8 weeks of age the mutants develop rear limb paralysis and many die by 8 weeks of age. The phenotypic characteristics of this mutation are similar to the original mutation spontaneous fracture (Gulo^sfx ) except for the eye phenotype described below that is inherent in the background strain on which the sfx^2J mutation arose. Mice homozygous for the sfx^2J remutation also had cataracts and rosettes and wavy outer nuclear layer of retinas. The eyes of a female mutant were checked using an opthalmascope and it was found to have cataracts on both eyes (as the strain background has characteristically). It has not yet been determined if the rosettes and wavy outer nuclear layer of the retinas is also characteristic of the background strain.

Development

Mus domesticus trapped in Val Poschiavo were found to have seven Robertsonian translocations. One male descended from this stock was bred with a female from the random-bred Swiss NMRI/Han stock. These F1 offspring were sent from Alfred Gropp at Medizinische Hochschule in Lubeck to Thomas Roderick at The Jackson Laboratory where they were intercrossed and lines were separated at the F2 generation for inbreeding. One of the recombinant inbred lines that resulted was RB156BNR/Ei which is homozygous for Rb(1.3)1Bnr, Rb(8.12)5Bnr, and Rb(9.14)6Bnr. When RB156BNR/Ei reached F?+64 in 1989, a mutation causing a sparse, curly haired phenotype was observed, named ruffled (rul), and the mutant strain was maintained segregating for rul. In 2002 when this mutant strain was at F?+64+47 the spontaneous fracture 2 Jackson mutation arose spontaneously in this strain. It has been maintained via progeny testing to identify heterozygous breeders.