NOD.129-(D19Mit10-D19Mit54)/GseJ

Stock number: 005352
Product name: NOD.Ins1¹²⁹ control
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

These mice carry Chromosome 19 alleles from strain 129S1/SvImJ at the Ins1 loci and are useful as controls for congenic strain NOD.129S2(B6)-Ins1^tm1Jja/GseJ in studies relating to diabetes.

Detailed description

This NOD/ShiLt congenic strain, commonly called NOD.Ins1 control, is carrying Chromosome 19 alleles derived from strain 129S1/SvImJ at the Ins1 loci. Diabetes onset and incidence among NOD.129-(D19Mit10-D19Mit54)/GseJ congenic mice is similar to NOD, Nakayama M, Nature 2005 435:220-3.

This strain is useful as a control for the exploration of Ins1 in the context of the NOD congenic Ins1 targeted mutation (Stock No. 005035).

Development

A Chr. 19 genomic segment extending from D19Mit10 (47cM) through D19Mit54 (52cM) that includes the Ins1 loci (49cM) was transferred from 129S1/SvImJ to NOD/Bdc using speed congenic techniques. (Nakayama M, Nature 2005 435:220-3). In 2005, the Type 1 Diabetes Resource (T1DR) received heterozygous mice at N9. Using microsatellite analysis mice carrying the D19Mit10 (47cM) - D19Mit54 (52cM) segment from 129S1/SVImJ were intercrossed to make a homozygous strain. Microsatellite analysis, performed at The Jackson Laboratory additionally, indicates D19Mit53 (43cM) and D19Mit137 (55.7cM) are 129S1/SvImJ in origin while D19Mit90 (41cM) is of NOD/Bdc origin. Additional microsatellite analysis confirms all known Idd alleles (1-20) are NOD/Bdc in origin.

Allele

MGI accession ID: MGI:96572
Marker symbol: Ins1
Marker name: insulin I
Marker synonyms: IDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10; PNDM4
Chromosome: 19

Allele

MGI accession ID: MGI:91216
Marker symbol: D19Mit10
Marker name: DNA segment, Chr 19, Massachusetts Institute of Technology 10
Chromosome: 19

Allele

MGI accession ID: MGI:91266
Marker symbol: D19Mit54
Marker name: DNA segment, Chr 19, Massachusetts Institute of Technology 54
Chromosome: 19