These mice carry Chromosome 19 alleles from strain 129S1/SvImJ at the Ins1 loci and are useful as controls for congenic strain NOD.129S2(B6)-Ins1tm1Jja/GseJ in studies relating to diabetes.
George Eisenbarth, U of Colorado
Genetic Background | Generation |
---|---|
|
Marker Symbol | Marker Name | |
---|---|---|
D19Mit54 | DNA segment, Chr 19, Massachusetts Institute of Technology 54 | |
D19Mit10 | DNA segment, Chr 19, Massachusetts Institute of Technology 10 | |
Ins1 | insulin I |
This NOD/ShiLt congenic strain, commonly called NOD.Ins1 control, is carrying Chromosome 19 alleles derived from strain 129S1/SvImJ at the Ins1 loci. Diabetes onset and incidence among NOD.129-(D19Mit10-D19Mit54)/GseJ congenic mice is similar to NOD, Nakayama M, Nature 2005 435:220-3.
This strain is useful as a control for the exploration of Ins1 in the context of the NOD congenic Ins1 targeted mutation (Stock No. 005035).
A Chr. 19 genomic segment extending from D19Mit10 (47cM) through D19Mit54 (52cM) that includes the Ins1 loci (49cM) was transferred from 129S1/SvImJ to NOD/Bdc using speed congenic techniques. (Nakayama M, Nature 2005 435:220-3). In 2005, the Type 1 Diabetes Resource (T1DR) received heterozygous mice at N9. Using microsatellite analysis mice carrying the D19Mit10 (47cM) - D19Mit54 (52cM) segment from 129S1/SVImJ were intercrossed to make a homozygous strain. Microsatellite analysis, performed at The Jackson Laboratory additionally, indicates D19Mit53 (43cM) and D19Mit137 (55.7cM) are 129S1/SvImJ in origin while D19Mit90 (41cM) is of NOD/Bdc origin. Additional microsatellite analysis confirms all known Idd alleles (1-20) are NOD/Bdc in origin.
A footpad injection of Complete Freund s Adjuvant (CFA) administered once at weaning will delay diabetes onset, thus extending the lifespan of breeders. Use of Complete Freund s Adjuvant in NOD mice can be found in Current Protocols in Immunology page 15.9.19, Reproduction.
When using the NOD.Ins1129 control mouse strain in a publication, please cite the originating article(s) and include JAX stock #005352 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous, 1 pair minimum |
Frozen Mouse Embryo | NOD.129-(D19Mit10-D19Mit54)/GseJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.129-(D19Mit10-D19Mit54)/GseJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.129-(D19Mit10-D19Mit54)/GseJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | NOD.129-(D19Mit10-D19Mit54)/GseJ Frozen Embryos | $3373.50 |
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