awag homozygotes have a distinct tremor when walking. When lifted by the tail these mutant mice do not splay their hind legs out as normal mice do, but instead hold their legs in a bowlegged umbrella manner and arch their backs. Both sexes breed and live a normal life span.
The awag mutation arose spontaneously in 1998 on the C57BL/6J-Tyrc-2J/J background (Stock No. 000058)in the production facility of The Jackson Laboratory where it was discovered by Jay Wellington. This mutant subline has been maintained by sibling mating ever since and this strain is still homozygous for Tyrc-2J although maintained segregating for awag.
|Allele Name||albino 2 Jackson|
|Allele Synonym(s)||albino 2 Jackson; Tyrc-2J|
|Gene Symbol and Name||Tyr, tyrosinase|
|Gene Synonym(s)||albino; ATN; c; CMM8; skin/coat color 35; OCA1; OCA1A; OCAIA; skc35; skc35; SHEP3; C; Oca1|
|Site of Expression||Expressed in melanosomes|
|Strain of Origin||B6.Cg-Tyrp1b Hps1ep|
|Molecular Note||This mutation has a G to T base change at nucleotide 291 resulting in an amino acid change from arginine to leucine at residue 77 which lies in the highly conserved DDRE sequence. Nucleotide 291 is at the alternative 5' splice donor site for exon 1 and this allele does not produce the 1a or 1b subset of tyrosinase transcripts but does produce a significant increase in 1c and 1d transcripts. Western blots of homozygous mutant skin extracts demostrate the nearly complete absence of the broad 76-84 kDa band of glycosylated wild-type tyrosinase. No tyrosinase activity was found in hairbulb extracts from homozygous mice.|
|Allele Name||ages with abnormal gait|
|Allele Synonym(s)||ages with abnormal gait; Golga1awag|
|Gene Symbol and Name||Golga1, golgi autoantigen, golgin subfamily a, 1|
|Gene Synonym(s)||golgin-97; 0710001G09Rik; 0710001G09Rik; RIKEN cDNA 2210418B03 gene; 2210418B03Rik; 2210418B03Rik; Golgi97; expressed sequence AW107649; RIKEN cDNA 0710001G09 gene; AW107649; awag; awag; ages with abnormal gait|
|Strain of Origin||B6(Cg)-Tyrc-2J/J|
|Molecular Note||This spontaneous mutation was discovered in 1998 at The Jackson Laboratory. The molecular lesion is a small intragenic deletion in the gene.|
When using the ages with abnormal gait mouse strain in a publication, please cite the originating article(s) and include JAX stock #005349 in your Materials and Methods section.
|Heterozygous or wildtype for Golga1<awag>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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