Mice with this spontaneous mutation are characterized by walking with a tremor and splaying their hind legs when picked up.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous (Null/Knockout) | Tyr | tyrosinase |
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Golga1 | golgi autoantigen, golgin subfamily a, 1 |
awag homozygotes have a distinct tremor when walking. When lifted by the tail these mutant mice do not splay their hind legs out as normal mice do, but instead hold their legs in a bowlegged umbrella manner and arch their backs. Both sexes breed and live a normal life span.
The awag mutation arose spontaneously in 1998 on the C57BL/6J-Tyrc-2J/J background (Stock No. 000058)in the production facility of The Jackson Laboratory where it was discovered by Jay Wellington. This mutant subline has been maintained by sibling mating ever since and this strain is still homozygous for Tyrc-2J although maintained segregating for awag.
Allele Name | albino 2 Jackson |
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Allele Type | Spontaneous (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Tyr, tyrosinase |
Gene Synonym(s) | |
Site of Expression | Expressed in melanosomes |
Strain of Origin | B6.Cg-Tyrp1b Hps1ep |
Chromosome | 7 |
Molecular Note | This mutation has a G-to-T base change at coding nucleotide 230 resulting in an amino acid change from arginine to leucine at residue 77 (p.R77L) which lies in the highly conserved DDRE sequence. Nucleotide 230 is at the alternative 5' splice donor site for exon 1 and this allele does not produce the 1a or 1b subset of tyrosinase transcripts but does produce a significant increase in 1c and 1d transcripts. Western blots of homozygous mutant skin extracts demostrate the nearly complete absence of the broad 76-84 kDa band of glycosylated wild-type tyrosinase. No tyrosinase activity was found in hairbulb extracts from homozygous mice. |
Allele Name | ages with abnormal gait |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Golga1, golgi autoantigen, golgin subfamily a, 1 |
Gene Synonym(s) | |
Strain of Origin | B6(Cg)-Tyrc-2J/J |
Chromosome | 2 |
Molecular Note | This spontaneous mutation was discovered in 1998 at The Jackson Laboratory. The molecular lesion is a small intragenic deletion of TTCACAC at 39,023,425-431 (GRCm38) in the gene. This causes a frame shift and premature termination. |
When using the ages with abnormal gait mouse strain in a publication, please cite the originating article(s) and include JAX stock #005349 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Golga1<awag> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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