NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J

Stock number: 005334
Product name: NOD.CD4-GFP line 1
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

This strain is useful for tracking resting and activated T cells in vivo and in vitro.

Detailed description

Homozygous transgenic mice are viable, fertile, normal in size and do not exhibit any gross physical or behavioral abnormalities. The donating investigator reports uniform transgenic expression, about 81% of inactivated or resting CD8⁺ T cells and CD4⁺ T cells, with minimal expression in B cells (2.3%) and macrophages (1.5%). Over a three day period following activation in vitro with anti-CD3, gating on live populations of CD4⁺ and CD8⁺ subsets show continued GFP expression in both. However, there is a greater diminution of GFP fluorescence in the activated CD8⁺ T population by day three in vitro. Diabetes onset is similar to wild-type NOD/ShiLt mice. These CD4-GFP transgenic mice may be useful for fluorescent monitoring of T cells both in vivo and in vitro.

Development

A transgenic construct containing the enhanced green fluorescent protein, EGFP, cDNA under the control of the murine Cd4 promoter and enhancer was injected into fertilized NOD/ShiLt mouse eggs. Founder line 1 was mated to NOD/ShiLt prior to sibling mating. In 2007, the T1DR received this transgenic strain at N2F4.

Allele

Symbol: Tg(Cd4-EGFP)1Lt
Name: transgene insertion 1, Edward Leiter
MGI accession ID: MGI:3720219
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Cd4-EGFP)1Lt
Marker name: transgene insertion 1, Edward Leiter
Chromosome: UN
Strain of origin: NOD/ShiLt
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: FACs analysis has detected expression of the transgene in 81% of inactivated or resting CD8⁺ T cells and CD4⁺ T cells, with minimal expression in B cells (2.3%) and macrophages (1.5%).
Molecular note: A construct containing an enhanced green florescent protein cDNA fused to the murine Cd4 promoter and enhancer was injected into NOD/ShiLt fertilized embryos. T-cells glow green. 80% of T-cells show GFP expression by FACS while B-cells and macrophages do not express the transgene.