This strain is useful for tracking resting and activated T cells in vivo and in vitro.
Dr. Edward Leiter, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Homozygous transgenic mice are viable, fertile, normal in size and do not exhibit any gross physical or behavioral abnormalities. The donating investigator reports uniform transgenic expression, about 81% of inactivated or resting CD8+ T cells and CD4+ T cells, with minimal expression in B cells (2.3%) and macrophages (1.5%). Over a three day period following activation in vitro with anti-CD3, gating on live populations of CD4+ and CD8+ subsets show continued GFP expression in both. However, there is a greater diminution of GFP fluorescence in the activated CD8+ T population by day three in vitro. Diabetes onset is similar to wild-type NOD/ShiLt mice. These CD4-GFP transgenic mice may be useful for fluorescent monitoring of T cells both in vivo and in vitro.
A transgenic construct containing the enhanced green fluorescent protein, EGFP, cDNA under the control of the murine Cd4 promoter and enhancer was injected into fertilized NOD/ShiLt mouse eggs. Founder line 1 was mated to NOD/ShiLt prior to sibling mating. In 2007, the T1DR received this transgenic strain at N2F4.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | FACs analysis has detected expression of the transgene in 81% of inactivated or resting CD8+ T cells and CD4+ T cells, with minimal expression in B cells (2.3%) and macrophages (1.5%). |
Allele Name | transgene insertion 1, Edward Leiter |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Cd4-GFP line 1; Cd4-GFP line1 |
Gene Symbol and Name | Tg(Cd4-EGFP)1Lt, transgene insertion 1, Edward Leiter |
Gene Synonym(s) | |
Promoter | Cd4, Cd4 molecule, rat |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | FACs analysis has detected expression of the transgene in 81% of inactivated or resting CD8+ T cells and CD4+ T cells, with minimal expression in B cells (2.3%) and macrophages (1.5%). |
Strain of Origin | NOD/ShiLt |
Chromosome | UN |
Molecular Note | A construct containing an enhanced green florescent protein cDNA fused to the murine Cd4 promoter and enhancer was injected into NOD/ShiLt fertilized embryos. T-cells glow green. 80% of T-cells show GFP expression by FACS while B-cells and macrophages do not express the transgene. |
Mutations Made By | Dr. Edward Leiter, The Jackson Laboratory |
When using the NOD.CD4-GFP line 1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005334 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Tg(Cd4-EGFP)1Lt, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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