B6.129-Cdh1^tm2Kem/J

Stock number: 005319
Product name: Cdh1 flox , E-cadherin flox
Research areas: Cell Biology Research, Developmental Biology Research, Research Tools

Description

When mice of this strain are bred to a strain expressing Cre recombinase in specific tissues, this strain may useful in studies of various epithelial cell functions.

Detailed description

These mice possess loxP sites flanking exons 6 to 10 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

Development

A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted into intron 10 of the targeted gene, and another loxP site was inserted into intron 5. This construct was electroporated into 129 derived R1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice and then backcrossed to the same for 10 generations before being made homozygous.

Allele

Symbol: Cdh1^tm2Kem
Name: targeted mutation 2, Rolf Kemler
MGI accession ID: MGI:2389020
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Cdh1
Marker name: cadherin 1
Chromosome: 8
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Single loxP sites flanked a region encompassing exons 6 through 10 followed by a floxed selection cassette was excised via in vitro cre mediated recombination.