When mice of this strain are bred to a strain expressing Cre recombinase in specific tissues, this strain may useful in studies of various epithelial cell functions.
Prof. Dr. RolfK Kemler, Max Planck Institute for Immunobiology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Cdh1 | cadherin 1 |
These mice possess loxP sites flanking exons 6 to 10 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted into intron 10 of the targeted gene, and another loxP site was inserted into intron 5. This construct was electroporated into 129 derived R1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice and then backcrossed to the same for 10 generations before being made homozygous.
Allele Name | targeted mutation 2, Rolf Kemler |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Ecadfl; E-cadlox; E-cadherinflox; E-cadherinfloxed |
Gene Symbol and Name | Cdh1, cadherin 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 8 |
Molecular Note | Single loxP sites flanked a region encompassing exons 6 through 10 followed by a floxed selection cassette was excised via in vitro cre mediated recombination. |
Mutations Made By | Wilhelmine (Mimi) de Vries, The Jackson Laboratory |
This strain is maintained as a homozygote.
When using the Cdh1 flox , E-cadherin flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #005319 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Cdh1<tm2Kem> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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