Overview

Also Known As:clone 4 TCR

These transgenic mice express a T-cell receptor specific for an epitope of hemagglutinin that is restricted by MHC class I H2-K^d.

Donating Investigator

Linda Sherman, The Scripps Research Institute

Genetic overview

Genetic Background	Generation
000463 B10.D2-Hc¹ H2^d H2-T18^c/nSnJ

Hc¹

Allele Type	Gene Symbol	Gene Name
Not Applicable	Hc	hemolytic complement

H2^d

Allele Type	Gene Symbol	Gene Name
Spontaneous	H2	histocompatibility-2, MHC

Tg(TcraCl4,TcrbCl4)1Shrm

Allele Type
Transgenic (Inserted expressed sequence)

Marker(s)

	Marker Symbol	Marker Name
	H2-T18	histocompatibility 2, T region locus 18

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Research Applications

Research Tools
Immunology, Inflammation and Autoimmunity Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Transgenic mice are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities.The TCR expressed from this transgene is specific for influenza virus A/PR/8 hemagglutinin (HA) in the context of the MHC class I molecule H2-K^d. Both thymic and peripheral T-cell populations are skewed toward CD8+ cells. The majority of thymocytes and virtually all CD8+ T cells in lymph nodes express the transgenic TCR beta chain. About 40% of peripheral blood CD8+ T cells react with the HA peptide presented by H2-K^d. When mated with Tg(Ins2-HA)165Bri, double transgenic neonates have similar levels of V-beta 8 and total number of thymocytes as Tg(TcraCl4,TcrbCl4) mice however the double transgenics become spontaneously diabetic after birth and die within 10 days.

This transgenic model is useful in the study of T-cell activation, cross presentation of antigens, process of thymic selection, peripheral tolerance and the immune response to influenza.

Development

The Tcra and Tcrb transgenes encode a Tcr cDNA from Clone 4 derived from a B10.D2-Hc¹ H2^d H2-T18^c/nSn mouse previously immunized with Influenza virus A/PR/8. The clone 4 transgenic constructs were co-injected and co-inserted in (C57BL/6 x BALB/c)F1 oocytes. Transgene positive mice were backcrossed to B10.D2-Hc¹ H2^d H2-T18^c for 20 generations. In 2005, The Jackson Laboratory received B10.Cg-H2^d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ (Clone4 TCR) at generation N20 and backcrossed it to B10.D2-Hc¹ H2^d H2-T18^c/nSnJ (Stock No. 000463) one generation prior to maintaining by brother-sister matings.

Expression Data

Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcra, T cell receptor alpha chain, mouse, laboratory
Site of Expression	T-cells.

Control Suggestions

Noncarrier

Additional Information

Considerations for Choosing Controls

Selected References

Morgan DJ; Liblau R; Scott B; Fleck S; McDevitt HO; Sarvetnick N; Lo D; Sherman LA. 1996. CD8(+) T cell-mediated spontaneous diabetes in neonatal mice. J Immunol 157(3):978-83PubMed: 8757600 MGI: J:99756

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Genetics

Hc¹

Allele Symbol: Hc¹

Allele Name	sufficient
Allele Type	Not Applicable
Allele Synonym(s)	C5 sufficient; C5-s; C5-suf
Gene Symbol and Name	Hc, hemolytic complement
Gene Synonym(s)
Strain of Origin	multiple strains
Chromosome	2
General Note	Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The C3H/HeJ allele does not have the 2 bp deletion and is associated with resistance to allergen-induced bronchial hyperresponsiveness. (J:108211)
Molecular Note	This allele does not contain the 2 base "TA" deletion found in the Hc⁰ allele and is found in the following mouse strains BALB/cJ, C57BL/6J, DBA/1J, and B10.D2/nSnJ. This is equivalent to the wild-type allele.

H2^d

Allele Symbol: H2^d

Allele Name	d variant
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	H2, histocompatibility-2, MHC
Gene Synonym(s)
Strain of Origin	various
Chromosome	17
General Note	The d variant has been observed in the following strains: DBA/2, DBA/2J BALB/c, BALB/cByJ, BALB/cJ, C57BLKS, NZB.

Tg(TcraCl4,TcrbCl4)1Shrm

Allele Symbol: Tg(TcraCl4,TcrbCl4)1Shrm

Allele Name	transgene insertion 1, Linda Sherman
Allele Type	Transgenic (Inserted expressed sequence)
Allele Synonym(s)	(HA)-TCR; C4; CL4; CL-4; CL-4 TCR; CL4-TCR; Clone 4; clone 4 TCR; Clone-4 TCR
Gene Symbol and Name	Tg(TcraCl4,TcrbCl4)1Shrm, transgene insertion 1, Linda Sherman
Gene Synonym(s)
Promoter	Tcrb, T cell receptor beta chain, mouse, laboratory
Promoter	Tcra, T cell receptor alpha chain, mouse, laboratory
Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcra, T cell receptor alpha chain, mouse, laboratory
Site of Expression	T-cells.
Strain of Origin	(C57BL/6 x BALB/c)F1
Chromosome	UN
Molecular Note	Tcra and Tcrb cDNAs were isolated from Clone-4, a CTL line derived from a B10.D2-Hc¹ H2^d H2-t18^c/nSnJ mouse immunized with influenza virus A/PR/8. A Tcra cDNA fragment representing the V-J exon, containing Valpha10, was cloned into a Tcra shuttle vector that includes a mouse Tcra-V promoter, the immunoglobulin heavy-chain enhancer E_H, and the Tcra-C genes with some flanking DNA. Likewise, a Tcrb cDNA fragment representing the VDJ exon, containing Vbeta8.2, was cloned into a Tcrb shuttle vector including a mouse Tcrb-V promoter, E_H, and the Tcrb-C genes with some flanking DNA. The plasmid constructs were coinjected. This transgenic TCR specifically recognizes influenza virus A/PR/8 hemagglutinin restricted by MHC class I H2k^d.
Mutations Made By	Linda Sherman, The Scripps Research Institute

H2-T18

Marker Symbol: H2-T18

Marker Synonym(s)
Chromosome(s)	17

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T Cell Receptor Transgenics
- Diabetes and Obesity Research
- Cancer Research
  - tumor immunology
Immunology, Inflammation and Autoimmunity Research
- Rearranged Antigen-Specific T Cell Receptor Transgenes

Genotype: H2^d related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genotype: Hc¹ related

Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - specific complement deficiency, control
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - specific complement deficiency, C5 complement, control

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Tg(TcraCl4,TcrbCl4)1Shrm/?
B10.Cg-H2 Tg(TcraCl4,TcrbCl4)1Shrm

hematopoietic system phenotype

abnormal CD8-positive, alpha-beta T cell differentiation
- the majority of mature CD8 T cells in the periphery express the transgenic T cell receptor (TCR) that is specific for influenza virus hemagglutinin (HA) peptide presented by MHC-Class I H2-K^d
- 40% of the CD8 T cells in the blood are reactive to HA peptide presented by H2-K^d
- most thymocytes also express the transgenic TCR

abnormal T cell subpopulation ratio
- skewing toward the development of single-positive CD8 T cells is evident in the thymus
- there are 4-fold more CD8 T cells than CD4 T cells found in the lymph nodes

immune system phenotype

abnormal CD8-positive, alpha-beta T cell differentiation
- 40% of the CD8 T cells in the blood are reactive to HA peptide presented by H2-K^d
- the majority of mature CD8 T cells in the periphery express the transgenic T cell receptor (TCR) that is specific for influenza virus hemagglutinin (HA) peptide presented by MHC-Class I H2-K^d
- most thymocytes also express the transgenic TCR

abnormal T cell subpopulation ratio
- there are 4-fold more CD8 T cells than CD4 T cells found in the lymph nodes
- skewing toward the development of single-positive CD8 T cells is evident in the thymus

References

Morgan DJ; Liblau R; Scott B; Fleck S; McDevitt HO; Sarvetnick N; Lo D; Sherman LA. 1996. CD8(+) T cell-mediated spontaneous diabetes in neonatal mice. J Immunol 157(3):978-83PubMed: 8757600 MGI: J:99756

Additional - H2^d related

Additional - H2-T18 related

Additional - Hc¹ related

Additional - Tg(TcraCl4,TcrbCl4)1Shrm related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Tg(TcraCl4,TcrbCl4)1Shrm
- Probe:Tg(TcraCl4,TcrbCl4)1Shrm-Probe
- Genotyping resources and troubleshooting
Mating System
- +/+ sibling x Hemizygote
Appearance
- black
  Related Genotype: a/a
Citation
When using the clone 4 TCR mouse strain in a publication, please cite the originating article(s) and include JAX stock #005308 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Hemizygous or Non carrier for Tg(TcraCl4,TcrbCl4)1Shrm

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:clone 4 TCR

Donating Investigator

Genetic overview

Marker(s)

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Hc1

Allele Symbol: Hc1

H2d

Allele Symbol: H2d

Tg(TcraCl4,TcrbCl4)1Shrm

Allele Symbol: Tg(TcraCl4,TcrbCl4)1Shrm

H2-T18

Marker Symbol: H2-T18

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: H2d related

Genotype: Hc1 related

Mammalian Phenotype Terms by Genotype

Genotype: Tg(TcraCl4,TcrbCl4)1Shrm/?B10.Cg-H2 Tg(TcraCl4,TcrbCl4)1Shrm

hematopoietic system phenotype

immune system phenotype

References

Additional - H2d related

Additional - H2-T18 related

Additional - Hc1 related

Additional - Tg(TcraCl4,TcrbCl4)1Shrm related

Genotyping Protocols

Mating System

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Hc¹

Allele Symbol: Hc¹

H2^d

Allele Symbol: H2^d

Genotype: H2^d related

Genotype: Hc¹ related

Genotype: Tg(TcraCl4,TcrbCl4)1Shrm/?
B10.Cg-H2 Tg(TcraCl4,TcrbCl4)1Shrm

Additional - H2^d related

Additional - Hc¹ related