These transgenic mice express a T-cell receptor specific for an epitope of hemagglutinin that is restricted by MHC class I H2-Kd.
Linda Sherman, The Scripps Research Institute
Genetic Background | Generation |
---|---|
000463 B10.D2-Hc1 H2d H2-T18c/nSnJ |
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Not Applicable | Hc | hemolytic complement |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | H2 | histocompatibility-2, MHC |
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Marker Symbol | Marker Name | |
---|---|---|
H2-T18 | histocompatibility 2, T region locus 18 |
Transgenic mice are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities.The TCR expressed from this transgene is specific for influenza virus A/PR/8 hemagglutinin (HA) in the context of the MHC class I molecule H2-Kd. Both thymic and peripheral T-cell populations are skewed toward CD8+ cells. The majority of thymocytes and virtually all CD8+ T cells in lymph nodes express the transgenic TCR beta chain. About 40% of peripheral blood CD8+ T cells react with the HA peptide presented by H2-Kd. When mated with Tg(Ins2-HA)165Bri, double transgenic neonates have similar levels of V-beta 8 and total number of thymocytes as Tg(TcraCl4,TcrbCl4) mice however the double transgenics become spontaneously diabetic after birth and die within 10 days.
This transgenic model is useful in the study of T-cell activation, cross presentation of antigens, process of thymic selection, peripheral tolerance and the immune response to influenza.
The Tcra and Tcrb transgenes encode a Tcr cDNA from Clone 4 derived from a B10.D2-Hc1 H2d H2-T18c/nSn mouse previously immunized with Influenza virus A/PR/8. The clone 4 transgenic constructs were co-injected and co-inserted in (C57BL/6 x BALB/c)F1 oocytes. Transgene positive mice were backcrossed to B10.D2-Hc1 H2d H2-T18c for 20 generations. In 2005, The Jackson Laboratory received B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ (Clone4 TCR) at generation N20 and backcrossed it to B10.D2-Hc1 H2d H2-T18c/nSnJ (Stock No. 000463) one generation prior to maintaining by brother-sister matings.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
---|---|
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression | T-cells. |
Allele Name | sufficient |
---|---|
Allele Type | Not Applicable |
Allele Synonym(s) | C5 sufficient; C5-s; C5-suf |
Gene Symbol and Name | Hc, hemolytic complement |
Gene Synonym(s) | |
Strain of Origin | multiple strains |
Chromosome | 2 |
General Note | Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The C3H/HeJ allele does not have the 2 bp deletion and is associated with resistance to allergen-induced bronchial hyperresponsiveness. (J:108211) |
Molecular Note | This allele does not contain the 2 base "TA" deletion found in the Hc0 allele and is found in the following mouse strains BALB/cJ, C57BL/6J, DBA/1J, and B10.D2/nSnJ. This is equivalent to the wild-type allele. |
Allele Name | d variant |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | H2, histocompatibility-2, MHC |
Gene Synonym(s) | |
Strain of Origin | various |
Chromosome | 17 |
General Note | The d variant has been observed in the following strains: DBA/2, DBA/2J BALB/c, BALB/cByJ, BALB/cJ, C57BLKS, NZB. |
Allele Name | transgene insertion 1, Linda Sherman |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | (HA)-TCR; C4; CL4; CL-4; CL-4 TCR; CL4-TCR; Clone 4; clone 4 TCR; Clone-4 TCR |
Gene Symbol and Name | Tg(TcraCl4,TcrbCl4)1Shrm, transgene insertion 1, Linda Sherman |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression | T-cells. |
Strain of Origin | (C57BL/6 x BALB/c)F1 |
Chromosome | UN |
Molecular Note | Tcra and Tcrb cDNAs were isolated from Clone-4, a CTL line derived from a B10.D2-Hc1 H2d H2-t18c/nSnJ mouse immunized with influenza virus A/PR/8. A Tcra cDNA fragment representing the V-J exon, containing Valpha10, was cloned into a Tcra shuttle vector that includes a mouse Tcra-V promoter, the immunoglobulin heavy-chain enhancer EH, and the Tcra-C genes with some flanking DNA. Likewise, a Tcrb cDNA fragment representing the VDJ exon, containing Vbeta8.2, was cloned into a Tcrb shuttle vector including a mouse Tcrb-V promoter, EH, and the Tcrb-C genes with some flanking DNA. The plasmid constructs were coinjected. This transgenic TCR specifically recognizes influenza virus A/PR/8 hemagglutinin restricted by MHC class I H2kd. |
Mutations Made By | Linda Sherman, The Scripps Research Institute |
Marker Synonym(s) | |
---|---|
Chromosome(s) | 17 |
When using the clone 4 TCR mouse strain in a publication, please cite the originating article(s) and include JAX stock #005308 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(TcraCl4,TcrbCl4)1Shrm |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.