A fraction of these transgenic mice show increased insulitis, and diabetes incidence is partially restored in females.
F. Susan Wong, Cardiff University School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ighm | immunoglobulin heavy constant mu |
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Transgenic mice carrying the Ighmtm1Cgn allele are viable, fertile, and normal in size and restores the B220+ B-cells to normal. The number of B-cells is normal and the ratio of CD4 to CD8 is normal. Although only a fraction of the transgenic mice have an increased amount of insulitis, it is more severe, especially in males. Diabetes incidence is partially restored in NODCaj.Cg-Ighmtm1Cgn Tg(Igh-VB1-8/Igh-6m)1Mjsk/Fsw with approximately, 11% females and 0% males becoming diabetic by 50 weeks of age compared to approximately 2% Ighmtm1Cgn female controls and 0% Ighmtm1Cgn male controls by 50 weeks of age or 90% female NOD controls and 70% male NOD controls by 30 weeks of age. T- cell depleted spleen cells from Ighmtm1Cgn Tg(Igh-VB1-8/Igh-6m)1Mjsk mice stimulated with lipopolysaccharide (LPS) respond normally. NODCaj.Cg-Ighmtm1Cgn Tg(Igh-VB1-8/Igh-6m )2Mjsk/Fsw like NOD.129S2(B6)-Ighmtm1Cgn generate low T-cell responses to GAD and insulin.
This model is useful for separating the role of B-cells in antigen presentation from antibody production involved in diabetes development.
The transgenic construct Tg(Igh-VB1-8/Igh-6m)1Mjsk that encodes a membrane bound, but not a secreted, immunoglobulin mu heavy chain, was injected into NOD/Caj zygotes. Founders were backcrossed to NOD/Caj. An outcross-intercross strategy was employed to introduce an inactivated Ighm allele from NOD.129S2(B6)-Ighmtm1Cgn. In 2005, the Type 1 Diabetes Resource received NOD/Caj.Cg-Ighmtm1Cgn Tg(Igh-VB1-8/Igh-6m)1Mjsk/FswJ at generation N12F3.
Expressed Gene | Ighm, immunoglobulin heavy constant mu, mouse, laboratory |
---|---|
Site of Expression |
Allele Name | targeted mutation 1, University of Cologne |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | B cell-; BCKO; BCR-; BKO; Cmu -; Ig- muMT; Ig-; IgH-; IgHmuMT; Igh-6-; Igh-6null; Igh-6tm1Cgn; Igh-6tm1CgnmuMT0; Ighm-; Igmunull; muIgKO; mum-; muMT; muMt-; mu-MT- |
Gene Symbol and Name | Ighm, immunoglobulin heavy constant mu |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 12 |
Molecular Note | A neomycin resistance cassette disrupted one of the membrane exons of the gene encoding immunoglobulin heavy chain of the class mu (IgM). |
Mutations Made By | Daisuke Kitamura, University of Cologne |
Allele Name | transgene insertion 1, Mark J Shlomchik |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | mIg; Tg(Igh-VB1-8/Igh-6)1Mjsk |
Gene Symbol and Name | Tg(Igh-VB1-8/Igh-6m)1Mjsk, transgene insertion 1, Mark J Shlomchik |
Gene Synonym(s) | |
Promoter | Igh-V, immunoglobulin heavy chain variable region, mouse, laboratory |
Expressed Gene | Ighm, immunoglobulin heavy constant mu, mouse, laboratory |
Strain of Origin | NOD/Caj |
Chromosome | UN |
Molecular Note | The transgene encodes a membrane-bound, but not a secreted, immunoglobulin mu heavy chain. The variable region of the transgene comprises the VDJ moiety from the functionally rearranged Igh gene of the C57BL/6-derived B1-8 hybridoma, containing the V-region segment Vh186.2 and recognizing the hapten (4-hydroxy-3-nitrophenyl)acetyl. This is followed by a J-C intron fragment containing the Igh intronic enhancer and an Igh-6 constant region gene (of haplotype a) from which the exon encoding the secreted form of Igmu and its associated polyadenylation signal have been deleted. |
Mutations Made By | Mark Shlomchik, Yale School of Medicine |
When using the NOD.mIgM mouse strain in a publication, please cite the originating article(s) and include JAX stock #005306 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Igh-6<tm1Cgn> , Hemizygous or Homozygous for Tg(Igh-VB1-8/Igh-6m)1Mjsk, Tg/? |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.