This is an NIH subline of C57BL/6. It was separated from C57BL/6J in 1951. Five SNP differences have been identified that distinguish C57BL/6J from C57BL/6ByJ and C57BL/6NJ. This strain does not have the deletion in the Nnt gene that has been found in the C57BL/6J strain (Stock No. 000664).Read More +
This strain is homozygous for Crb1rd8, the retinal degeneration 8 mutation. Photoreceptor degeneration is observed in spots, caused by retinal folds and pseudorosettes, found in the fundus of the eye.
This is an NIH subline of C57BL/6. It was separated from C57BL/6J in 1951. 5 SNP differences have been identified that distinguish C57BL/6J from C57BL/6ByJ and C57BL/6NJ. Both C57BL/6ByJ and C57BL/6NJ type as follows: 08-015199792-M (rs3709624) is C; 11-004367508-M (rs3659787) is A; 13-041017317-M (rs3722313) is C; 15-057561875-M (rs3702158) is G; 19-049914266-M (rs3724876) is T. C57BL/6J types as follows: 08-015199792-M is T; 11-004367508-M is G; 13-041017317-M is T; 15-057561875-M is A; 19-049914266-M is G (Petkov and Wiles 2005.) This strain does not have the deletion in the Nnt gene that has been found in the C57BL/6J strain (Stock No. 000664). C57BL/6NJ mice are homozygous for Cyfip2M1N, a spontaneous mutation in the cytoplasmic FMR1 interacting protein 2 that results in an amino acid substitution of phenylalanine for serine at position 968 (S968F). The mutation is found in all C57BL/6N substrains but is not present in the C57BL/6J strain or substrains. The mutation results in 45% lower acute response to cocaine as measured by locomotor hyperactivity.
In 1951 C57BL/6J, then at generation F32, were sent from The Jackson Laboratory to The National Institute of Health where they were maintained via sibling mating for decades. In 1980 this subline reached F126 and in 1984 embryos were cryopreserved at the NIH cryopreservation facility. In approximately 1994 some of these embryos were thawed and maintained via sibling mating and this subline was referred to as B6(e84) to specify that it derived from the embryos frozen in 1984. In September and October of 1997 a new set of embryos were cryopreserved from this thawed line by backcrossing +F6 females to their +F5 fathers. In 2005 some of these embryos frozen in 1997 were sent to The Jackson Laboratory where they were thawed and gave rise to C57BL/6NJ. Thus, C57BL/6NJ is devoid of mutations that may have arisen in any C57BL/6N sublines that remained on the shelf after the 1984 freeze. The embryos received by The Jackson Laboratory were thawed, the colony expanded, and re-frozen immediately. This strain is maintained with frequent replenishment from this frozen stock in order to arrest genetic drift.
|Allele Name||retinal degeneration 8|
|Allele Synonym(s)||Rd8-; nmf144|
|Gene Symbol and Name||Crb1, crumbs family member 1, photoreceptor morphogenesis associated|
|Gene Synonym(s)||7530426H14Rik; 7530426H14Rik; A930008G09Rik; A930008G09Rik; LCA8; RIKEN cDNA 7530426H14 gene; RIKEN cDNA A930008G09 gene; RP12|
|Strain of Origin||C57BL/6By or C57BL/6N|
|Molecular Note||The mutation in the rd8 mouse has been identified as a single base deletion in the Crb1 gene. This deletion causes a frame shift and a premature stop codon that truncates the transmembrane and cytoplasmic domain of the protein. This mutation has been found to be present in all sublines of C57BL/6N and in C57BL/6ByJ, but not in any C57BL/6J subline. It occurred sometime between transfer of mice from JAX to NIH, in 1951, and from NIH to Donald Bailey, in 1961.|
|Allele Name||mutation 1, National Institutes of Health|
|Gene Symbol and Name||Cyfip2, cytoplasmic FMR1 interacting protein 2|
|Gene Synonym(s)||1500004I01Rik; 1500004I01Rik; 6430511D02Rik; 6430511D02Rik; AA930218; AU022376; PIR121; Pir121; RIKEN cDNA 1500004I01 gene; RIKEN cDNA 6430511D02 gene; expressed sequence AA930218; expressed sequence AU022376; mKIAA1168|
|Strain of Origin||C57BL/6N|
|Molecular Note||An G to A mutation at 46,036,117 base pair (bp) of chromosome 11 (mm9) occured between 1961 and 1974 in the C57BL/6N strain that resulted in the amino acid substitution of phenylalanine for serine at position 968 (S968F). This mutation is found in all C57BL/6N substrains (C57BL/6NCrl, C57BL/6NTac and C57BL/6NJ) but is not present in the C57BL/6J strain or substrains (C57BL/6Ei, C57BL/6EiJ, C57BL/6ByJ and C57BL/6By). The mutation destabilizes the protein leading to decreased half-life.|
|Allele Type||Spontaneous (Not Applicable)|
|Gene Symbol and Name||Nlrp12, NLR family, pyrin domain containing 12|
|Gene Synonym(s)||CLR19.3; FCAS2; NACHT, LRR and PYD containing protein 12; NALP12; Nalp12; Nalp12; PAN6; PYPAF7; RNO; RNO2|
|Strain of Origin||C57BL/6N|
|Molecular Note||A sequence variant is identified in C57BL/6N (C) as compared to C57BL/6J (T) at position 3,222,538 (version mm9-NCB1m37). The polymorphism is located in the C-terminal leucine-rich repeat domain and results in a lysine to arginine change at amino acid residue 1034.|
When using the C57BL/6NJ mouse strain in a publication, please include JAX stock #005304 in your Materials and Methods section.
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