Overview

Also Known As:B6N, Black 6N

This is an NIH subline of C57BL/6. It was separated from C57BL/6J in 1951. Five SNP differences have been identified that distinguish C57BL/6J from C57BL/6ByJ and C57BL/6NJ. This strain does not have the deletion in the Nnt gene that has been found in the C57BL/6J strain (Stock No. 000664).

Genetic overview

Genetic Background	Generation

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Research Applications

Research Tools
Developmental Biology Research
Sensorineural Research
Cell Biology Research
Mouse/Human Gene Homologs

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Details

Important Note

This strain is homozygous for Crb1^rd8, the retinal degeneration 8 mutation. Photoreceptor degeneration is observed in spots, caused by retinal folds and pseudorosettes, found in the fundus of the eye.

Detailed Description

This is an NIH subline of C57BL/6. It was separated from C57BL/6J in 1951. 5 SNP differences have been identified that distinguish C57BL/6J from C57BL/6ByJ and C57BL/6NJ. Both C57BL/6ByJ and C57BL/6NJ type as follows: 08-015199792-M (rs3709624) is C; 11-004367508-M (rs3659787) is A; 13-041017317-M (rs3722313) is C; 15-057561875-M (rs3702158) is G; 19-049914266-M (rs3724876) is T. C57BL/6J types as follows: 08-015199792-M is T; 11-004367508-M is G; 13-041017317-M is T; 15-057561875-M is A; 19-049914266-M is G (Petkov and Wiles 2005.) This strain does not have the deletion in the Nnt gene that has been found in the C57BL/6J strain (Stock No. 000664). C57BL/6NJ mice are homozygous for Cyfip2^M1N, a spontaneous mutation in the cytoplasmic FMR1 interacting protein 2 that results in an amino acid substitution of phenylalanine for serine at position 968 (S968F). The mutation is found in all C57BL/6N substrains but is not present in the C57BL/6J strain or substrains. The mutation results in 45% lower acute response to cocaine as measured by locomotor hyperactivity (Kumar et al., 2013). This strain is also homozygous for the Del(13)1N deletion, common to the NIH sublines, which includes Zfp998 and Zfp997 and has been found to cause the loss of transcriptional repression of non-ecotropic endogenous retroviruses in C57BL/6NJ compared with C57BL/6J, which does not have this deletion (Treger et al., 2019).

Development

In 1951 C57BL/6J, then at generation F32, were sent from The Jackson Laboratory to The National Institute of Health where they were maintained via sibling mating for decades. In 1980 this subline reached F126 and in 1984 embryos were cryopreserved at the NIH cryopreservation facility. In approximately 1994 some of these embryos were thawed and maintained via sibling mating and this subline was referred to as B6(e84) to specify that it derived from the embryos frozen in 1984. In September and October of 1997 a new set of embryos were cryopreserved from this thawed line by backcrossing +F6 females to their +F5 fathers. In 2005 some of these embryos frozen in 1997 were sent to The Jackson Laboratory where they were thawed and gave rise to C57BL/6NJ. Thus, C57BL/6NJ is devoid of mutations that may have arisen in any C57BL/6N sublines that remained on the shelf after the 1984 freeze. The embryos received by The Jackson Laboratory were thawed, the colony expanded, and re-frozen immediately. This strain is maintained with frequent replenishment from this frozen stock in order to arrest genetic drift.

Selected References

Fontaine DA; Davis DB. 2016. Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium. Diabetes 65(1):25-33PubMed: 26696638 MGI: J:231066
Kapur M; Ganguly A; Nagy G; Adamson SI; Chuang JH; Frankel WN; Ackerman SL. 2020. Expression of the Neuronal tRNA n-Tr20 Regulates Synaptic Transmission and Seizure Susceptibility. Neuron 108(1):193-208.e9PubMed: 32853550 MGI: J:297745
Keane TM; Goodstadt L; Danecek P; White MA; Wong K; Yalcin B; Heger A; Agam A; Slater G; Goodson M; Furlotte NA; Eskin E; Nellaker C; Whitley H; Cleak J; Janowitz D; Hernandez-Pliego P; Edwards A; Belgard TG; Oliver PL; McIntyre RE; Bhomra A; Nicod J; Gan X; Yuan W; van der Weyden L; Steward CA; Bala S; Stalker J; Mott R; Durbin R; Jackson IJ; Czechanski A; Guerra-Assuncao JA; Donahue LR; Reinholdt LG; Payseur BA; Ponting CP; Birney E; Flint J; Adams DJ. 2011. Mouse genomic variation and its effect on phenotypes and gene regulation. Nature 477(7364):289-94PubMed: 21921910 MGI: J:177037
Kumar V; Kim K; Joseph C; Kourrich S; Yoo SH; Huang HC; Vitaterna MH; de Villena FP; Churchill G; Bonci A; Takahashi JS. 2013. C57BL/6N mutation in Cytoplasmic FMRP interacting protein 2 regulates cocaine response. Science 342(6165):1508-12PubMed: 24357318 MGI: J:203183
Simon MM; Greenaway S; White JK; Fuchs H; Gailus-Durner V; Wells S; Sorg T; Wong K; Bedu E; Cartwright EJ; Dacquin R; Djebali S; Estabel J; Graw J; Ingham NJ; Jackson IJ; Lengeling A; Mandillo S; Marvel J; Meziane H; Preitner F; Puk O; Roux M; Adams DJ; Atkins S; Ayadi A; Becker L; Blake A; Brooker D; Cater H; Champy MF; Combe R; Danecek P; di Fenza A; Gates H; Gerdin AK; Golini E; Hancock JM; Hans W; Holter SM; Hough T; Jurdic P; Keane TM; Morgan H; Muller W; Neff F; Nicholson G; Pasche B; Roberson LA; R. 2013. A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains. Genome Biol 14(7):R82PubMed: 23902802 MGI: J:220417
Timmermans S; Van Montagu M; Libert C. 2017. Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains. Proc Natl Acad Sci U S A 114(34):9158-9163PubMed: 28784771 MGI: J:244295
Treger RS; Pope SD; Kong Y; Tokuyama M; Taura M; Iwasaki A. 2019. The Lupus Susceptibility Locus Sgp3 Encodes the Suppressor of Endogenous Retrovirus Expression SNERV. Immunity 50(2):334-347.e9PubMed: 30709743 MGI: J:282526

View All References

Genetics

Crb1^rd8

Allele Symbol: Crb1^rd8

Allele Name	retinal degeneration 8
Allele Type	Spontaneous
Allele Synonym(s)	Crb1^6N; nmf144; Rd8^-
Gene Symbol and Name	Crb1, crumbs family member 1, photoreceptor morphogenesis associated
Gene Synonym(s)
Strain of Origin	C57BL/6By or C57BL/6N
Chromosome	1
Molecular Note	The mutation in the rd8 mouse has been identified as a single base deletion of a C (G on forward strand) at coding nucleotide 3481 in the gene. This deletion causes a frame shift and a premature stop codon that truncates the transmembrane and cytoplasmic domain of the protein after amino acid 1207. This mutation has been found to be present in all sublines of C57BL/6N and in C57BL/6ByJ, but not in any C57BL/6J subline. It occurred sometime between transfer of mice from JAX to NIH, in 1951, and from NIH to Donald Bailey, in 1961.

Cyfip2^M1N

Allele Symbol: Cyfip2^M1N

Allele Name	mutation 1, National Institutes of Health
Allele Type	Spontaneous
Allele Synonym(s)	Cyfip2^6N; Cyfip2^B6N; Cyfip2^N
Gene Symbol and Name	Cyfip2, cytoplasmic FMR1 interacting protein 2
Gene Synonym(s)
Strain of Origin	C57BL/6N
Chromosome	11
Molecular Note	An G-to-A mutation at 46,222,615 bp of chromosome 11 (GRCm38) occurred between 1961 and 1974 in the C57BL/6N strain that resulted in the amino acid substitution of serine with phenylalanine at position 968 (p.S968F). This mutation is found in all C57BL/6N substrains (C57BL/6NCrl, C57BL/6NTac and C57BL/6NJ) but is not present in the C57BL/6J strain or substrains (C57BL/6Ei, C57BL/6EiJ, C57BL/6ByJ and C57BL/6By). The mutation destabilizes the protein leading to decreased half-life.

Nlrp12^C57BL/6N

Allele Symbol: Nlrp12^C57BL/6N

Allele Name	C57BL/6N
Allele Type	Spontaneous (Not Applicable)
Allele Synonym(s)	Nlrp12^B6N
Gene Symbol and Name	Nlrp12, NLR family, pyrin domain containing 12
Gene Synonym(s)
Strain of Origin	C57BL/6N
Chromosome	7
Molecular Note	A sequence variant is identified in C57BL/6N (C) as compared to C57BL/6J (T) at position 3,222,537 (GRCm38). The polymorphism is located in the C-terminal leucine-rich repeat domain and results in a lysine to arginine change at amino acid residue 1034 (p.K1034R).

Cox7a2l^s

Allele Symbol: Cox7a2l^s

Allele Name	short
Allele Type	Not Applicable (Not Specified)
Allele Synonym(s)	SCAF1¹¹¹
Gene Symbol and Name	Cox7a2l, cytochrome c oxidase subunit 7A2 like
Gene Synonym(s)
Strain of Origin	multiple strains
Chromosome	17
General Note	Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T⁺ Itpr3^tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ.
Molecular Note	This allele contains a 6 bp microdeletion causing the loss of two amino acids and truncating the protein to 111 amino acids instead of 113 amino acids. It is found in BALB/cAnCrl, BALB/cJ, C57BL/6Cr, C57BL/6JCrl, C57BL/6JOlaHsd, C57BL/6NCrl, C57BL/6NJcl, C57BL/6NHsd, C57BL/6NTac, C57BL/6NJ, B6(CG)-Tyr/J, C57BL/6JClr, C57BL/6JArc, C57BL/6JJcl, C57BL/6JJmsSlc, C57BL/6JOla, C57BL/6JOlaHsd, C57BL/6JRcc, C57BL/6JRccHsd, C57BL/6JBom, and C57BL/6JBomTac mice.

Del(13)1N

Allele Symbol: Del(13)1N

Allele Name	Deletion, Chr 13, NIH 1
Allele Type	Spontaneous (Modified regulatory region)
Allele Synonym(s)
Gene Symbol and Name	Del(13)1N, Deletion, Chr 13, NIH 1
Gene Synonym(s)
Strain of Origin	C57BL/6N
Chromosome	13
Molecular Note	This multigenic deletion, present in C57BL/6NJ and C57BL/6NTac but not C57BL/6J inbred strains, is approximately 1 Mb in size and spans from Chr13:65.66Mb-66.67Mb which includes several lincRNA and pseudogenes along with Zfp998 and Zfp997, with the distal breakpoint falling between Zfp997 and Zfp640.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- General Purpose

Genotype: Crb1^rd8 related

Developmental Biology Research
- Eye Defects
Sensorineural Research
- Retinal Degeneration
Cell Biology Research
- Defects in Cell Adhesion Molecules
Mouse/Human Gene Homologs
- Retinitis pigmentosa 12; Leber congenital amaurosis (CLA)

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Cdh23^ahl/Cdh23^ahl
C57BL/6NJ

hearing/vestibular/ear phenotype

cochlear outer hair cell degeneration

cochlear inner hair cell degeneration

cochlear hair cell degeneration
- degeneration of outer hair cells is more extensive than that of inner hair cells and losses of both are more severe in the base of the cochlea than in the apex

nervous system phenotype

cochlear inner hair cell degeneration

cochlear outer hair cell degeneration

cochlear hair cell degeneration
- degeneration of outer hair cells is more extensive than that of inner hair cells and losses of both are more severe in the base of the cochlea than in the apex

Phenotype Information

Body Weight Information - JAX^® Mice Strain C57BL/6NJ (005304)
Festing Inbred Strain Characteristics: C57BL

References

Fontaine DA; Davis DB. 2016. Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium. Diabetes 65(1):25-33PubMed: 26696638 MGI: J:231066
Kapur M; Ganguly A; Nagy G; Adamson SI; Chuang JH; Frankel WN; Ackerman SL. 2020. Expression of the Neuronal tRNA n-Tr20 Regulates Synaptic Transmission and Seizure Susceptibility. Neuron 108(1):193-208.e9PubMed: 32853550 MGI: J:297745
Keane TM; Goodstadt L; Danecek P; White MA; Wong K; Yalcin B; Heger A; Agam A; Slater G; Goodson M; Furlotte NA; Eskin E; Nellaker C; Whitley H; Cleak J; Janowitz D; Hernandez-Pliego P; Edwards A; Belgard TG; Oliver PL; McIntyre RE; Bhomra A; Nicod J; Gan X; Yuan W; van der Weyden L; Steward CA; Bala S; Stalker J; Mott R; Durbin R; Jackson IJ; Czechanski A; Guerra-Assuncao JA; Donahue LR; Reinholdt LG; Payseur BA; Ponting CP; Birney E; Flint J; Adams DJ. 2011. Mouse genomic variation and its effect on phenotypes and gene regulation. Nature 477(7364):289-94PubMed: 21921910 MGI: J:177037
Kumar V; Kim K; Joseph C; Kourrich S; Yoo SH; Huang HC; Vitaterna MH; de Villena FP; Churchill G; Bonci A; Takahashi JS. 2013. C57BL/6N mutation in Cytoplasmic FMRP interacting protein 2 regulates cocaine response. Science 342(6165):1508-12PubMed: 24357318 MGI: J:203183
Simon MM; Greenaway S; White JK; Fuchs H; Gailus-Durner V; Wells S; Sorg T; Wong K; Bedu E; Cartwright EJ; Dacquin R; Djebali S; Estabel J; Graw J; Ingham NJ; Jackson IJ; Lengeling A; Mandillo S; Marvel J; Meziane H; Preitner F; Puk O; Roux M; Adams DJ; Atkins S; Ayadi A; Becker L; Blake A; Brooker D; Cater H; Champy MF; Combe R; Danecek P; di Fenza A; Gates H; Gerdin AK; Golini E; Hancock JM; Hans W; Holter SM; Hough T; Jurdic P; Keane TM; Morgan H; Muller W; Neff F; Nicholson G; Pasche B; Roberson LA; R. 2013. A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains. Genome Biol 14(7):R82PubMed: 23902802 MGI: J:220417
Timmermans S; Van Montagu M; Libert C. 2017. Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains. Proc Natl Acad Sci U S A 114(34):9158-9163PubMed: 28784771 MGI: J:244295
Treger RS; Pope SD; Kong Y; Tokuyama M; Taura M; Iwasaki A. 2019. The Lupus Susceptibility Locus Sgp3 Encodes the Suppressor of Endogenous Retrovirus Expression SNERV. Immunity 50(2):334-347.e9PubMed: 30709743 MGI: J:282526

Additional References

Toye AA; Lippiat JD; Proks P; Shimomura K; Bentley L; Hugill A; Mijat V; Goldsworthy M; Moir L; Haynes A; Quarterman J; Freeman HC; Ashcroft FM; Cox RD. 2005. A genetic and physiological study of impaired glucose homeostasis control in C57BL/6J mice. Diabetologia 48(4):675-86PubMed: 15729571 MGI: J:106692
Ulland TK; Jain N; Hornick EE; Elliott EI; Clay GM; Sadler JJ; Mills KA; Janowski AM; Volk AP; Wang K; Legge KL; Gakhar L; Bourdi M; Ferguson PJ; Wilson ME; Cassel SL; Sutterwala FS. 2016. Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment. Nat Commun 7:13180PubMed: 27779193 MGI: J:238047

Additional - Cox7a2l^s related

Additional - Crb1^rd8 related

Additional - Cyfip2^M1N related

Additional - Nlrp12^C57BL/6N related

Additional - Del(13)1N related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:GAL Panel
- End Point Analysis:H2rs253049200-Alt 1-EP
Inbred mouse strains are maintained through sibling (sister x brother) matings; no genotyping required.

Breeding Considerations

This strain is a good breeder.

Additional Breeding and Husbandry Support

Mating System

Inbred x Inbred

Appearance

black
Related Genotype: a/a

Citation
When using the B6N mouse strain in a publication, please include JAX stock #005304 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

RB07 (Maximum)
MP13 (Maximum)
AX5 (Standard)
EM05 (Maximum)

Pricing & Availability

Readily Available

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International

Live Mouse

Age Genotype Price

weeks

Volume Pricing Details

QUANTITY Volume Pricing

Volume Pricing Program
Quantities: Volume pricing is automatically applied when a minimum quantity per strain for a shipment is reached
Sexes: Sexes of the same strain may be combined to reach minimum quantity levels to receive the volume pricing
Shipment: All shipping destinations qualify

Cryorecovery - Pricing

Service/Product Description Price

> > Inbred, 1 pair minimum will be supplied

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:B6N, Black 6N

Genetic overview

Research Applications

Base Price

Volume Pricing Available!

Important Note

Detailed Description

Development

Selected References

Crb1rd8

Allele Symbol: Crb1rd8

Cyfip2M1N

Allele Symbol: Cyfip2M1N

Nlrp12C57BL/6N

Allele Symbol: Nlrp12C57BL/6N

Cox7a2ls

Allele Symbol: Cox7a2ls

Del(13)1N

Allele Symbol: Del(13)1N

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Crb1rd8 related

Mammalian Phenotype Terms by Genotype

Genotype: Cdh23ahl/Cdh23ahlC57BL/6NJ

hearing/vestibular/ear phenotype

nervous system phenotype

Phenotype Information

References

Additional References

Additional - Cox7a2ls related

Additional - Crb1rd8 related

Additional - Cyfip2M1N related

Additional - Nlrp12C57BL/6N related

Additional - Del(13)1N related

Genotyping Protocols

Breeding Considerations

Mating System

Appearance

Citation

Animal Health Reports

Live Mouse

Volume Pricing Details

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Crb1^rd8

Allele Symbol: Crb1^rd8

Cyfip2^M1N

Allele Symbol: Cyfip2^M1N

Nlrp12^C57BL/6N

Allele Symbol: Nlrp12^C57BL/6N

Cox7a2l^s

Allele Symbol: Cox7a2l^s

Genotype: Crb1^rd8 related

Genotype: Cdh23^ahl/Cdh23^ahl
C57BL/6NJ

Additional - Cox7a2l^s related

Additional - Crb1^rd8 related

Additional - Cyfip2^M1N related

Additional - Nlrp12^C57BL/6N related