C57BL/6NJ

Stock No:: 005304

Also Known As: B6N, Black 6N

This is an NIH subline of C57BL/6. It was separated from C57BL/6J in 1951. Five SNP differences have been identified that distinguish C57BL/6J from C57BL/6ByJ and C57BL/6NJ. This strain does not have the deletion in the Nnt gene that has been found in the C57BL/6J strain (Stock No. 000664).

Genetic overview

Genetic Background	Generation

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Research Applications

Cell Biology Research
Developmental Biology Research
Sensorineural Research
Research Tools

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$20.83 Domestic price for male 3-week

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Details

Important Note

This strain is homozygous for Crb1^rd8, the retinal degeneration 8 mutation. Photoreceptor degeneration is observed in spots, caused by retinal folds and pseudorosettes, found in the fundus of the eye.

Detailed Description

This is an NIH subline of C57BL/6. It was separated from C57BL/6J in 1951. 5 SNP differences have been identified that distinguish C57BL/6J from C57BL/6ByJ and C57BL/6NJ. Both C57BL/6ByJ and C57BL/6NJ type as follows: 08-015199792-M (rs3709624) is C; 11-004367508-M (rs3659787) is A; 13-041017317-M (rs3722313) is C; 15-057561875-M (rs3702158) is G; 19-049914266-M (rs3724876) is T. C57BL/6J types as follows: 08-015199792-M is T; 11-004367508-M is G; 13-041017317-M is T; 15-057561875-M is A; 19-049914266-M is G (Petkov and Wiles 2005.) This strain does not have the deletion in the Nnt gene that has been found in the C57BL/6J strain (Stock No. 000664). C57BL/6NJ mice are homozygous for Cyfip2^M1N, a spontaneous mutation in the cytoplasmic FMR1 interacting protein 2 that results in an amino acid substitution of phenylalanine for serine at position 968 (S968F). The mutation is found in all C57BL/6N substrains but is not present in the C57BL/6J strain or substrains. The mutation results in 45% lower acute response to cocaine as measured by locomotor hyperactivity.

Development

In 1951 C57BL/6J, then at generation F32, were sent from The Jackson Laboratory to The National Institute of Health where they were maintained via sibling mating for decades. In 1980 this subline reached F126 and in 1984 embryos were cryopreserved at the NIH cryopreservation facility. In approximately 1994 some of these embryos were thawed and maintained via sibling mating and this subline was referred to as B6(e84) to specify that it derived from the embryos frozen in 1984. In September and October of 1997 a new set of embryos were cryopreserved from this thawed line by backcrossing +F6 females to their +F5 fathers. In 2005 some of these embryos frozen in 1997 were sent to The Jackson Laboratory where they were thawed and gave rise to C57BL/6NJ. Thus, C57BL/6NJ is devoid of mutations that may have arisen in any C57BL/6N sublines that remained on the shelf after the 1984 freeze. The embryos received by The Jackson Laboratory were thawed, the colony expanded, and re-frozen immediately. This strain is maintained with frequent replenishment from this frozen stock in order to arrest genetic drift.

Selected References

Keane TM; Goodstadt L; Danecek P; White MA; Wong K; Yalcin B; Heger A; Agam A; Slater G; Goodson M; Furlotte NA; Eskin E; Nellaker C; Whitley H; Cleak J; Janowitz D; Hernandez-Pliego P; Edwards A; Belgard TG; Oliver PL; McIntyre RE; Bhomra A; Nicod J; Gan X; Yuan W; van der Weyden L; Steward CA; Bala S; Stalker J; Mott R; Durbin R; Jackson IJ; Czechanski A; Guerra-Assuncao JA; Donahue LR; Reinholdt LG; Payseur BA; Ponting CP; Birney E; Flint J; Adams DJ. 2011. Mouse genomic variation and its effect on phenotypes and gene regulation. (7364): 289-94. PubMed: 21921910 MGI: 178133
Kumar V; Kim K; Joseph C; Kourrich S; Yoo SH; Huang HC; Vitaterna MH; de Villena FP; Churchill G; Bonci A; Takahashi JS. 2013. C57BL/6N mutation in Cytoplasmic FMRP interacting protein 2 regulates cocaine response. (6165): 1508-12. PubMed: 24357318 MGI: 204280
Fontaine DA; Davis DB. 2016. Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium. (1): 25-33. PubMed: 26696638 MGI: 232160
Simon MM; Greenaway S; White JK; Fuchs H; Gailus-Durner V; Wells S; Sorg T; Wong K; Bedu E; Cartwright EJ; Dacquin R; Djebali S; Estabel J; Graw J; Ingham NJ; Jackson IJ; Lengeling A; Mandillo S; Marvel J; Meziane H; Preitner F; Puk O; Roux M; Adams DJ; Atkins S; Ayadi A; Becker L; Blake A; Brooker D; Cater H; Champy MF; Combe R; Danecek P; di Fenza A; Gates H; Gerdin AK; Golini E; Hancock JM; Hans W; Holter SM; Hough T; Jurdic P; Keane TM; Morgan H; Muller W; Neff F; Nicholson G; Pasche B; Roberson LA; R. 2013. A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains. (7): R82. PubMed: 23902802 MGI: 221514
Timmermans S; Van Montagu M; Libert C. 2017. Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains. (34): 9158-9163. PubMed: 28784771 MGI: 245385

View All References

Genetics

Crb1^rd8

Allele Symbol: Crb1^rd8

Allele Name	retinal degeneration 8
Allele Type	Spontaneous
Allele Synonym(s)	Rd8^-; nmf144
Gene Symbol and Name	Crb1, crumbs family member 1, photoreceptor morphogenesis associated
Gene Synonym(s)	7530426H14Rik; A930008G09Rik; RIKEN cDNA 7530426H14 gene; RIKEN cDNA A930008G09 gene; LCA8; 7530426H14Rik; RP12; A930008G09Rik
Strain of Origin	C57BL/6By or C57BL/6N
Chromosome	1
Molecular Note	The mutation in the rd8 mouse has been identified as a single base deletion at nt3481 in the gene. This deletion causes a frame shift and a premature stop codon that truncates the transmembrane and cytoplasmic domain of the protein after amino acid 1207. This mutation has been found to be present in all sublines of C57BL/6N and in C57BL/6ByJ, but not in any C57BL/6J subline. It occurred sometime between transfer of mice from JAX to NIH, in 1951, and from NIH to Donald Bailey, in 1961.

Cyfip2^M1N

Allele Symbol: Cyfip2^M1N

Allele Name	mutation 1, National Institutes of Health
Allele Type	Spontaneous
Allele Synonym(s)	Cyfip2^B6N
Gene Symbol and Name	Cyfip2, cytoplasmic FMR1 interacting protein 2
Gene Synonym(s)	AU022376; expressed sequence AA930218; 6430511D02Rik; 1500004I01Rik; RIKEN cDNA 6430511D02 gene; expressed sequence AU022376; AA930218; 1500004I01Rik; PIR121; mKIAA1168; 6430511D02Rik; RIKEN cDNA 1500004I01 gene; Pir121; EIEE65
Strain of Origin	C57BL/6N
Chromosome	11
Molecular Note	An G to A mutation at 46,036,117 base pair (bp) of chromosome 11 (mm9) occured between 1961 and 1974 in the C57BL/6N strain that resulted in the amino acid substitution of phenylalanine for serine at position 968 (S968F). This mutation is found in all C57BL/6N substrains (C57BL/6NCrl, C57BL/6NTac and C57BL/6NJ) but is not present in the C57BL/6J strain or substrains (C57BL/6Ei, C57BL/6EiJ, C57BL/6ByJ and C57BL/6By). The mutation destabilizes the protein leading to decreased half-life.

Nlrp12^C57BL/6N

Allele Symbol: Nlrp12^C57BL/6N

Allele Name	C57BL/6N
Allele Type	Spontaneous
Allele Synonym(s)	Nlrp12^B6N
Gene Symbol and Name	Nlrp12, NLR family, pyrin domain containing 12
Gene Synonym(s)	FCAS2; Nalp12; NALP12; PYPAF7; PAN6; RNO2; RNO; CLR19.3; NACHT, LRR and PYD containing protein 12; Nalp12
Strain of Origin	C57BL/6N
Chromosome	7
Molecular Note	A sequence variant is identified in C57BL/6N (C) as compared to C57BL/6J (T) at position 3,222,538 (version mm9-NCB1m37). The polymorphism is located in the C-terminal leucine-rich repeat domain and results in a lysine to arginine change at amino acid residue 1034.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Crb1^rd8 related

Cell Biology Research
- Defects in Cell Adhesion Molecules
Developmental Biology Research
- Eye Defects
Sensorineural Research
- Retinal Degeneration

Research Tools
- General Purpose

Mammalian Phenotype Terms by Genotype

Genotype: Cdh23^ahl/Cdh23^ahl
C57BL/6NJ

hearing/vestibular/ear phenotype

cochlear hair cell degeneration
- degeneration of outer hair cells is more extensive than that of inner hair cells and losses of both are more severe in the base of the cochlea than in the apex

cochlear inner hair cell degeneration

cochlear outer hair cell degeneration

nervous system phenotype

cochlear hair cell degeneration
- degeneration of outer hair cells is more extensive than that of inner hair cells and losses of both are more severe in the base of the cochlea than in the apex

cochlear inner hair cell degeneration

cochlear outer hair cell degeneration

Phenotype Information

Body Weight Information - JAX^® Mice Strain C57BL/6NJ (005304)

Festing Inbred Strain Characteristics: C57BL

References

Keane TM; Goodstadt L; Danecek P; White MA; Wong K; Yalcin B; Heger A; Agam A; Slater G; Goodson M; Furlotte NA; Eskin E; Nellaker C; Whitley H; Cleak J; Janowitz D; Hernandez-Pliego P; Edwards A; Belgard TG; Oliver PL; McIntyre RE; Bhomra A; Nicod J; Gan X; Yuan W; van der Weyden L; Steward CA; Bala S; Stalker J; Mott R; Durbin R; Jackson IJ; Czechanski A; Guerra-Assuncao JA; Donahue LR; Reinholdt LG; Payseur BA; Ponting CP; Birney E; Flint J; Adams DJ. 2011. Mouse genomic variation and its effect on phenotypes and gene regulation. (7364): 289-94. PubMed: 21921910 MGI: 178133
Kumar V; Kim K; Joseph C; Kourrich S; Yoo SH; Huang HC; Vitaterna MH; de Villena FP; Churchill G; Bonci A; Takahashi JS. 2013. C57BL/6N mutation in Cytoplasmic FMRP interacting protein 2 regulates cocaine response. (6165): 1508-12. PubMed: 24357318 MGI: 204280
Fontaine DA; Davis DB. 2016. Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium. (1): 25-33. PubMed: 26696638 MGI: 232160
Simon MM; Greenaway S; White JK; Fuchs H; Gailus-Durner V; Wells S; Sorg T; Wong K; Bedu E; Cartwright EJ; Dacquin R; Djebali S; Estabel J; Graw J; Ingham NJ; Jackson IJ; Lengeling A; Mandillo S; Marvel J; Meziane H; Preitner F; Puk O; Roux M; Adams DJ; Atkins S; Ayadi A; Becker L; Blake A; Brooker D; Cater H; Champy MF; Combe R; Danecek P; di Fenza A; Gates H; Gerdin AK; Golini E; Hancock JM; Hans W; Holter SM; Hough T; Jurdic P; Keane TM; Morgan H; Muller W; Neff F; Nicholson G; Pasche B; Roberson LA; R. 2013. A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains. (7): R82. PubMed: 23902802 MGI: 221514
Timmermans S; Van Montagu M; Libert C. 2017. Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains. (34): 9158-9163. PubMed: 28784771 MGI: 245385

Additional References

Toye AA; Lippiat JD; Proks P; Shimomura K; Bentley L; Hugill A; Mijat V; Goldsworthy M; Moir L; Haynes A; Quarterman J; Freeman HC; Ashcroft FM; Cox RD. 2005. A genetic and physiological study of impaired glucose homeostasis control in C57BL/6J mice. (4): 675-86. PubMed: 15729571 MGI: 107766
Ulland TK; Jain N; Hornick EE; Elliott EI; Clay GM; Sadler JJ; Mills KA; Janowski AM; Volk AP; Wang K; Legge KL; Gakhar L; Bourdi M; Ferguson PJ; Wilson ME; Cassel SL; Sutterwala FS. 2016. Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment. 13180. PubMed: 27779193 MGI: 239139

Additional - Crb1^rd8

Chang B; Hawes NL; Hurd RE; Davisson MT; Nusinowitz S; Heckenlively JR. 2002. Retinal degeneration mutants in the mouse. (4): 517-25. PubMed: 11853768 MGI: 76050
Mehalow AK; Kameya S; Smith RS; Hawes NL; Denegre JM; Young JA; Bechtold L; Haider NB; Tepass U; Heckenlively JR; Chang B; Naggert JK; Nishina PM. 2003. CRB1 is essential for external limiting membrane integrity and photoreceptor morphogenesis in the mammalian retina. (17): 2179-89. PubMed: 12915475 MGI: 86437
Vessey KA; Greferath U; Jobling AI; Phipps JA; Ho T; Waugh M; Fletcher EL. 2012. Ccl2/Cx3cr1 knockout mice have inner retinal dysfunction but are not an accelerated model of AMD. (12): 7833-46. PubMed: 23074204 MGI: 215313
Chang B; Hawes NL; Hurd RE; Wang J; Howell D; Davisson MT; Roderick TH; Nusinowitz S; Heckenlively JR. 2005. Mouse models of ocular diseases. (5): 587-93. PubMed: 16332269 MGI: 157466
Lakowski J; Baron M; Bainbridge J; Barber AC; Pearson RA; Ali RR; Sowden JC. 2010. Cone and rod photoreceptor transplantation in models of the childhood retinopathy Leber congenital amaurosis using flow-sorted Crx-positive donor cells. (23): 4545-59. PubMed: 20858907 MGI: 166670
Won J; Shi LY; Hicks W; Wang J; Hurd R; Naggert JK; Chang B; Nishina PM. 2011. Mouse model resources for vision research. 391384. PubMed: 21052544 MGI: 167775
Chen M; Glenn JV; Dasari S; McVicar C; Ward M; Colhoun L; Quinn M; Bierhaus A; Xu H; Stitt AW. 2014. RAGE regulates immune cell infiltration and angiogenesis in choroidal neovascularization. (2): e89548. PubMed: 24586862 MGI: 214916
Aleman TS; Cideciyan AV; Aguirre GK; Huang WC; Mullins CL; Roman AJ; Sumaroka A; Olivares MB; Tsai FF; Schwartz SB; Vandenberghe LH; Limberis MP; Stone EM; Bell P; Wilson JM; Jacobson SG. 2011. Human CRB1-associated retinal degeneration: comparison with the rd8 Crb1-mutant mouse model. (9): 6898-910. PubMed: 21757580 MGI: 182492
Mattapallil MJ; Wawrousek EF; Chan CC; Zhao H; Roychoudhury J; Ferguson TA; Caspi RR. 2012. The Rd8 mutation of the Crb1 gene is present in vendor lines of C57BL/6N mice and embryonic stem cells, and confounds ocular induced mutant phenotypes. (6): 2921-7. PubMed: 22447858 MGI: 183449
Hippert C; Graca AB; Barber AC; West EL; Smith AJ; Ali RR; Pearson RA. 2015. Muller glia activation in response to inherited retinal degeneration is highly varied and disease-specific. (3): e0120415. PubMed: 25793273 MGI: 234530
Barber AC; Hippert C; Duran Y; West EL; Bainbridge JW; Warre-Cornish K; Luhmann UF; Lakowski J; Sowden JC; Ali RR; Pearson RA. 2013. Repair of the degenerate retina by photoreceptor transplantation. (1): 354-9. PubMed: 23248312 MGI: 193617
Perkins GA; Scott R; Perez A; Ellisman MH; Johnson JE; Fox DA. 2012. Bcl-xL-mediated remodeling of rod and cone synaptic mitochondria after postnatal lead exposure: electron microscopy, tomography and oxygen consumption. 3029-48. PubMed: 23288995 MGI: 193683
Zhang N; Kolesnikov AV; Jastrzebska B; Mustafi D; Sawada O; Maeda T; Genoud C; Engel A; Kefalov VJ; Palczewski K. 2013. Autosomal recessive retinitis pigmentosa E150K opsin mice exhibit photoreceptor disorganization. (1): 121-37. PubMed: 23221340 MGI: 195254
Chang B; Hurd R; Wang J; Nishina P. 2013. Survey of common eye diseases in laboratory mouse strains. (7): 4974-81. PubMed: 23800770 MGI: 200013
Datta P; Allamargot C; Hudson JS; Andersen EK; Bhattarai S; Drack AV; Sheffield VC; Seo S. 2015. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. (32): E4400-9. PubMed: 26216965 MGI: 227607
Chen Y; Sawada O; Kohno H; Le YZ; Subauste C; Maeda T; Maeda A. 2013. Autophagy protects the retina from light-induced degeneration. (11): 7506-18. PubMed: 23341467 MGI: 197988
Luhmann UF; Carvalho LS; Holthaus SM; Cowing JA; Greenaway S; Chu CJ; Herrmann P; Smith AJ; Munro PM; Potter P; Bainbridge JW; Ali RR. 2015. The severity of retinal pathology in homozygous Crb1rd8/rd8 mice is dependent on additional genetic factors. (1): 128-41. PubMed: 25147295 MGI: 217610
Low BE; Krebs MP; Joung JK; Tsai SQ; Nishina PM; Wiles MV. 2014. Correction of the Crb1rd8 allele and retinal phenotype in C57BL/6N mice via TALEN-mediated homology-directed repair. (1): 387-95. PubMed: 24346171 MGI: 207886
Kohno H; Maeda T; Perusek L; Pearlman E; Maeda A. 2014. CCL3 production by microglial cells modulates disease severity in murine models of retinal degeneration. (8): 3816-27. PubMed: 24639355 MGI: 211098
Mirza M; Volz C; Karlstetter M; Langiu M; Somogyi A; Ruonala MO; Tamm ER; Jagle H; Langmann T. 2013. Correction: Progressive Retinal Degeneration and Glial Activation in the CLN6 Mouse Model of Neuronal Ceroid Lipofuscinosis: A Beneficial Effect of DHA and Curcumin Supplementation. (10): . PubMed: 24130650 MGI: 210223
Wang Y; Abu-Asab MS; Yu CR; Tang Z; Shen D; Tuo J; Li X; Chan CC. 2014. Platelet-derived growth factor (PDGF)-C inhibits neuroretinal apoptosis in a murine model of focal retinal degeneration. (6): 674-82. PubMed: 24709779 MGI: 212061
Luhmann UF; Carvalho LS; Robbie SJ; Cowing JA; Duran Y; Munro PM; Bainbridge JW; Ali RR. 2013. Ccl2, Cx3cr1 and Ccl2/Cx3cr1 chemokine deficiencies are not sufficient to cause age-related retinal degeneration. 80-7. PubMed: 23232206 MGI: 211529
Ardeljan D; Wang Y; Park S; Shen D; Chu XK; Yu CR; Abu-Asab M; Tuo J; Eberhart CG; Olsen TW; Mullins RF; White G; Wadsworth S; Scaria A; Chan CC. 2014. Interleukin-17 retinotoxicity is prevented by gene transfer of a soluble interleukin-17 receptor acting as a cytokine blocker: implications for age-related macular degeneration. (4): e95900. PubMed: 24780906 MGI: 216369
Herrmann P; Cowing JA; Cristante E; Liyanage SE; Ribeiro J; Duran Y; Abelleira Hervas L; Carvalho LS; Bainbridge JW; Luhmann UF; Ali RR. 2015. Cd59a deficiency in mice leads to preferential innate immune activation in the retinal pigment epithelium-choroid with age. (9): 2637-48. PubMed: 26234657 MGI: 227363
Greferath U; Vessey KA; Jobling AI; Mills SA; Bui BV; He Z; Nag N; Ohtsu H; Fletcher EL. 2014. The role of histamine in the retina: studies on the Hdc knockout mouse. (12): e116025. PubMed: 25545149 MGI: 226475
Ramkumar N; Harvey BM; Lee JD; Alcorn HL; Silva-Gagliardi NF; McGlade CJ; Bestor TH; Wijnholds J; Haltiwanger RS; Anderson KV. 2015. Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2. (10): e1005551. PubMed: 26496195 MGI: 228431
Sahu B; Chavali VR; Alapati A; Suk J; Bartsch DU; Jablonski MM; Ayyagari R. 2015. Presence of rd8 mutation does not alter the ocular phenotype of late-onset retinal degeneration mouse model. 273-84. PubMed: 25814825 MGI: 221921
Liegel RP; Ronchetti A; Sidjanin DJ. 2014. Alkylglycerone phosphate synthase (AGPS) deficient mice: models for rhizomelic chondrodysplasia punctate type 3 (RCDP3) malformation syndrome. 299-311. PubMed: 25197626 MGI: 225672
Schnabolk G; Stauffer K; O'Quinn E; Coughlin B; Kunchithapautham K; Rohrer B. 2014. A comparative analysis of C57BL/6J and 6N substrains; chemokine/cytokine expression and susceptibility to laser-induced choroidal neovascularization. 18-23. PubMed: 25305577 MGI: 231414
Samardzija M; Caprara C; Heynen SR; Willcox DeParis S; Meneau I; Traber G; Agca C; von Lintig J; Grimm C. 2014. A mouse model for studying cone photoreceptor pathologies. (8): 5304-13. PubMed: 25034607 MGI: 231216
Flynn E; Ueda K; Auran E; Sullivan JM; Sparrow JR. 2014. Fundus autofluorescence and photoreceptor cell rosettes in mouse models. (9): 5643-52. PubMed: 25015357 MGI: 231230
Boatright JH; Dalal N; Chrenek MA; Gardner C; Ziesel A; Jiang Y; Grossniklaus HE; Nickerson JM. 2015. Methodologies for analysis of patterning in the mouse RPE sheet. 40-60. PubMed: 25593512 MGI: 228676
Pak JS; Lee EJ; Craft CM. 2015. The retinal phenotype of Grk1-/- is compromised by a Crb1 rd8 mutation. 1281-94. PubMed: 26664249 MGI: 229384
Vessey KA; Waugh M; Jobling AI; Phipps JA; Ho T; Trogrlic L; Greferath U; Fletcher EL. 2015. Assessment of retinal function and morphology in aging Ccl2 knockout mice. (2): 1238-52. PubMed: 25626968 MGI: 232074
Borras T; Smith MH; Buie LK. 2015. A Novel Mgp-Cre Knock-In Mouse Reveals an Anticalcification/Antistiffness Candidate Gene in the Trabecular Meshwork and Peripapillary Scleral Region. (4): 2203-14. PubMed: 25711639 MGI: 232067
Dando SJ; Naranjo Golborne C; Chinnery HR; Ruitenberg MJ; McMenamin PG. 2016. A case of mistaken identity: CD11c-eYFP(+) cells in the normal mouse brain parenchyma and neural retina display the phenotype of microglia, not dendritic cells. (8): 1331-49. PubMed: 27189804 MGI: 233880
Nagai N; Ju M; Izumi-Nagai K; Robbie SJ; Bainbridge JW; Gale DC; Pierre E; Krauss AH; Adamson P; Shima DT; Ng YS. 2015. Novel CCR3 Antagonists Are Effective Mono- and Combination Inhibitors of Choroidal Neovascular Growth and Vascular Permeability. (9): 2534-49. PubMed: 26188133 MGI: 227166
Nagai N; Lundh von Leithner P; Izumi-Nagai K; Hosking B; Chang B; Hurd R; Adamson P; Adamis AP; Foxton RH; Ng YS; Shima DT. 2014. Spontaneous CNV in a novel mutant mouse is associated with early VEGF-A-driven angiogenesis and late-stage focal edema, neural cell loss, and dysfunction. (6): 3709-19. PubMed: 24845632 MGI: 231114
Wang X; Zhao L; Zhang J; Fariss RN; Ma W; Kretschmer F; Wang M; Qian HH; Badea TC; Diamond JS; Gan WB; Roger JE; Wong WT. 2016. Requirement for Microglia for the Maintenance of Synaptic Function and Integrity in the Mature Retina. (9): 2827-42. PubMed: 26937019 MGI: 232768
Ji X; Liu Y; Hurd R; Wang J; Fitzmaurice B; Nishina PM; Chang B. 2016. Retinal Pigment Epithelium Atrophy 1 (rpea1): A New Mouse Model With Retinal Detachment Caused by a Disruption of Protein Kinase C, theta. (3): 877-88. PubMed: 26978024 MGI: 239069
Ramkumar N; Omelchenko T; Silva-Gagliardi NF; McGlade CJ; Wijnholds J; Anderson KV. 2016. Crumbs2 promotes cell ingression during the epithelial-to-mesenchymal transition at gastrulation. (12): 1281-1291. PubMed: 27870829 MGI: 239643
Chen Q; Qiu F; Zhou K; Matlock HG; Takahashi Y; Rajala RVS; Yang Y; Moran E; Ma JX. 2017. Pathogenic Role of microRNA-21 in Diabetic Retinopathy Through Downregulation of PPARalpha. (6): 1671-1682. PubMed: 28270521 MGI: 254637
Feng L; Ju M; Lee KYV; Mackey A; Evangelista M; Iwata D; Adamson P; Lashkari K; Foxton R; Shima D; Ng YS. 2017. A Proinflammatory Function of Toll-Like Receptor 2 in the Retinal Pigment Epithelium as a Novel Target for Reducing Choroidal Neovascularization in Age-Related Macular Degeneration. (10): 2208-2221. PubMed: 28739342 MGI: 261275
Fontaine V; Monteiro E; Brazhnikova E; Lesage L; Balducci C; Guibout L; Feraille L; Elena PP; Sahel JA; Veillet S; Lafont R. 2016. Norbixin Protects Retinal Pigmented Epithelium Cells and Photoreceptors against A2E-Mediated Phototoxicity In Vitro and In Vivo. (12): e0167793. PubMed: 27992460 MGI: 262708
Lajko M; Cardona HJ; Taylor JM; Farrow KN; Fawzi AA. 2017. Photoreceptor oxidative stress in hyperoxia-induced proliferative retinopathy accelerates rd8 degeneration. (7): e0180384. PubMed: 28671996 MGI: 249992

Additional - Nlrp12^C57BL/6N

Ulland TK; Jain N; Hornick EE; Elliott EI; Clay GM; Sadler JJ; Mills KA; Janowski AM; Volk AP; Wang K; Legge KL; Gakhar L; Bourdi M; Ferguson PJ; Wilson ME; Cassel SL; Sutterwala FS. 2016. Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment. 13180. PubMed: 27779193 MGI: 239139

Technical Support

Contact Technical Support

Genotyping Protocols
- Separated PCR: GAL Panel
- Separated PCR: Nnt
- End Point Analysis: Crb1^rd8End Point
- End Point Analysis: Kif1a
- Separated MCA: Nnt
- Genotyping resources and troubleshooting
Inbred mouse strains are maintained through sibling (sister x brother) matings; no genotyping required.
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

This strain is a good breeder.
- Additional Breeding and Husbandry Support
Mating System
- Inbred x Inbred
Appearance
- black
  Related Genotype: a/a

Animal Health Reports

Facility Barrier Level Descriptions

Pricing & Availability

Readily Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Volume Pricing Details

QUANTITY	VOLUME PRICING

Volume Pricing Program
Quantities: Volume pricing is automatically applied when a minimum quantity per strain for a shipment is reached
Sexes: Sexes of the same strain may be combined to reach minimum quantity levels to receive the volume pricing
Shipment: All shipping destinations qualify

Cryorecovery - Pricing

Service	Genotype	Price
	Please inquire

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: B6N, Black 6N

Genetic overview

Research Applications

Base Price

Volume Pricing Available!

Important Note

Detailed Description

Development

Selected References

Crb1rd8

Allele Symbol: Crb1rd8

Cyfip2M1N

Allele Symbol: Cyfip2M1N

Nlrp12C57BL/6N

Allele Symbol: Nlrp12C57BL/6N

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Crb1rd8 related

Mammalian Phenotype Terms by Genotype

Genotype: Cdh23ahl/Cdh23ahlC57BL/6NJ

hearing/vestibular/ear phenotype

nervous system phenotype

Phenotype Information

References

Additional References

Additional - Crb1rd8

Additional - Nlrp12C57BL/6N

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Appearance

Animal Health Reports

Live Mouse

Volume Pricing Details

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Crb1^rd8

Allele Symbol: Crb1^rd8

Cyfip2^M1N

Allele Symbol: Cyfip2^M1N

Nlrp12^C57BL/6N

Allele Symbol: Nlrp12^C57BL/6N

Genotype: Crb1^rd8 related

Genotype: Cdh23^ahl/Cdh23^ahl
C57BL/6NJ

Additional - Crb1^rd8

Additional - Nlrp12^C57BL/6N