These Tulp3 knock-out mice exhibit failure of neural tube closure, and die by embryonic day 14.5. They are suitable for use in applications related to studies of sonic hedgehog (Shh) mediated signaling in embryonic development.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Tulp3 | tubby-like protein 3 |
The tubby-like protein 3 (Tulp3) gene has been identified as a member of a small novel gene family that is primarily neuronally expressed, and is involved in the sonic hedgehog (Shh) and modulation of the Shh signaling pathway. Embryos homozygous for the Tulp3tm1Jng mutant allele exhibit failure of neural tube closure, and die by embryonic day 14.5. Failure of cranial neural tube closure coincided with increased neuroepithelial apoptosis specifically in the hindbrain and the caudal neural tube. In addition, the number of betaIII-tubulin positive cells is significantly decreased in the hindbrain of Tulp3tm1Jng(-/-) embryos. Some Tulp3tm1Jng heterozygotes also manifest embryonic lethality with neuroepithelial cell death.
The targeting construct (described elsewhere) was electroporated into 129-derived R1 embryonic stem cells. Chimeric mice were crossed to 129S1/SvImJ and maintained by intercross. Embryos were cryopreserved using 129X1/SvJ oocyte donors.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Embryo |
Allele Name | targeted mutation 1, Jurgen Naggert |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Tulp3- |
Gene Symbol and Name | Tulp3, tubby-like protein 3 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Embryo |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 6 |
Molecular Note | A portion of exon 5 and exons 6-9 were removed and replaced with an in-frame GFP-neo construct via homologous recombination. The targeted mutation results in deletion of the C-terminus and part of the N-terminal region of the protein, and creates a fusion protein with GFP. Homozygous mutant embryos were identified by PCR genotype analysis. |
When using the Tulp3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005291 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Tulp3<tm1Jng> |
Frozen Mouse Embryo | 129-Tulp3<tm1Jng>/Pjn Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129-Tulp3<tm1Jng>/Pjn Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129-Tulp3<tm1Jng>/Pjn Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | 129-Tulp3<tm1Jng>/Pjn Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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