These Itgad knock-out mice exhibit reduced CD3 and CD28 expression levels, and CD4+ to CD8+ cell ratios.
Christie M. Ballantyne, Baylor College of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Itgad | integrin, alpha D |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by flow cytometry analysis of splenocytes and peripheral leucocytes. T cells from mutant mice exhibit reduced CD3 and CD28 expression levels, and CD4+ to CD8+ cell ratios. Superantigen (staphylococcal enterotoxin) challenge reveals impaired splenocyte response and T cell proliferation. This mutant mouse strain may be useful in studies of T cell development and proliferation, and leukocyte adhesion deficiency type I (LADI).
A targeting vector containing neomycin resistance gene driven by the mouse RNA polymerase II promoter was used to disrupt a 2.2 Kb region containing exons 1 and 2. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2 derived AB2.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 12 generations.
Allele Name | targeted mutation 1, Christie M Ballantyne |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD11d- |
Gene Symbol and Name | Itgad, integrin, alpha D |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 7 |
Molecular Note | A 2.2 kb genomic fragment containing exons 1 and 2 were replaced with a neomycin selection cassette. Flow cytometry demonstrated the absence of protein expression in splenocytes. |
Mutations Made By | Christie Ballantyne, Baylor College of Medicine |
When maintaining a live colony, these mice are bred as homozygotes.
When using the CD11d-KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005258 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Itgad<tm1Bll> |
Frozen Mouse Embryo | B6.129S7-Itgad<tm1Bll>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Itgad<tm1Bll>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Itgad<tm1Bll>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S7-Itgad<tm1Bll>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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