B6.129S7-Itgad^tm1Bll/J

Stock number: 005258
Product name: CD11d-KO
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

These Itgad knock-out mice exhibit reduced CD3 and CD28 expression levels, and CD4+ to CD8+ cell ratios.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by flow cytometry analysis of splenocytes and peripheral leucocytes. T cells from mutant mice exhibit reduced CD3 and CD28 expression levels, and CD4+ to CD8+ cell ratios. Superantigen (staphylococcal enterotoxin) challenge reveals impaired splenocyte response and T cell proliferation. This mutant mouse strain may be useful in studies of T cell development and proliferation, and leukocyte adhesion deficiency type I (LADI).

Development

A targeting vector containing neomycin resistance gene driven by the mouse RNA polymerase II promoter was used to disrupt a 2.2 Kb region containing exons 1 and 2. The construct was electroporated into 129S7/SvEvBrd-Hprt^b-m2 derived AB2.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 12 generations.

Allele

Symbol: Itgad^tm1Bll
Name: targeted mutation 1, Christie M Ballantyne
MGI accession ID: MGI:3579559
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Itgad
Marker name: integrin, alpha D
Chromosome: 7
Strain of origin: 129S7/SvEvBrd-Hprt1^b-m2
Molecular note: A 2.2 kb genomic fragment containing exons 1 and 2 were replaced with a neomycin selection cassette. Flow cytometry demonstrated the absence of protein expression in splenocytes.