Marker-assisted breeding was used to construct a set of overlapping congenic strains called genome-tagged mice (GTM) that span the entire mouse genome. Each member of the panel carries an average of a 23 cM segment (range 8 to 58 cM) from DBA/2J introgressed on to a C57BL/6J background. The segments represent the distal (D), middle (M), and proximal (P) portions of all or most of a given chromosome. GTM are a valuable resource for mapping and confirmation of loci contributing to complex traits. A more detailed explanation of the chromosome intervals are described in Iakoubova et al. 2001 Genomics 74:89 and Davis et al. 2005 Genomics 86:259. Dr. Lusis donated the panel to The Jackson Laboratory in 2004.
Stock No. 005188 is a C57BL/6J congenic strain carrying the middle portion of chromosome 13 from
DBA/2J. The Mit or SNP (rs) markers in the strain name define the
congenic interval.
In
2018, a 163 SNP (single nucleotide polymorphism) panel analysis
distinguishing between C57BL/6J and DBA/2J was performed on this mouse
line. Consistent with previous results, the DBA/2J congenic region on
chromosome 13 showed the proximal crossover point between 23.1-33.6 Mbp.
However, this analyses extends the most distal end of the DBA/2J
region: the most distal markers tested were DBA/2J allele-type (82.2,
85.1, 93.0 and 94.0 Mbp). Additionally, three markers within this region
were C57BL/6J allele-type (at 65.3, 65.9 and 66.8 Mbp), flanked by
DBA/2J allele-type markers proximally at 61.9 Mbp and distally at 77.8
Mbp. Taken together, this suggests these mice have two DBA/2J congenic
regions on chromosome 13: one spanning at least from 33.6-61.9 Mbp and
the other spanning at least from 77.8-94.0 Mbp.
