B6.D2-(D13Mit198-D13Mit314)/LusJ

Stock number: 005188
Research areas: Research Tools

Description

These congenic genome-tagged mice (GTM) were originally identified to carry a Chromosome 13 segment between markers Mit198 to Mit314 from the DBA/2 strain.

In 2018, a 163 SNP (single nucleotide polymorphism) panel analysis distinguishing between C57BL/6J and DBA/2J was performed on this mouse line. Consistent with previous results, the DBA/2J congenic region on chromosome 13 showed the proximal crossover point between 23.1-33.6 Mbp. However, this analyses extends the most distal end of the DBA/2J region: the most distal markers tested were DBA/2J allele-type (82.2, 85.1, 93.0 and 94.0 Mbp). Additionally, three markers within this region were C57BL/6J allele-type (at 65.3, 65.9 and 66.8 Mbp), flanked by DBA/2J allele-type markers proximally at 61.9 Mbp and distally at 77.8 Mbp. Taken together, this suggests these mice have two DBA/2J congenic regions on chromosome 13: one spanning at least from 33.6-61.9 Mbp and the other spanning at least from 77.8-94.0 Mbp.

Detailed description

Marker-assisted breeding was used to construct a set of overlapping congenic strains called genome-tagged mice (GTM) that span the entire mouse genome. Each member of the panel carries an average of a 23 cM segment (range 8 to 58 cM) from DBA/2J introgressed on to a C57BL/6J background. The segments represent the distal (D), middle (M), and proximal (P) portions of all or most of a given chromosome. GTM are a valuable resource for mapping and confirmation of loci contributing to complex traits. A more detailed explanation of the chromosome intervals are described in Iakoubova et al. 2001 Genomics 74:89 and Davis et al. 2005 Genomics 86:259. Dr. Lusis donated the panel to The Jackson Laboratory in 2004.

Stock No. 005188 is a C57BL/6J congenic strain carrying the middle portion of chromosome 13 from DBA/2J. The Mit or SNP (rs) markers in the strain name define the congenic interval.
In 2018, a 163 SNP (single nucleotide polymorphism) panel analysis distinguishing between C57BL/6J and DBA/2J was performed on this mouse line. Consistent with previous results, the DBA/2J congenic region on chromosome 13 showed the proximal crossover point between 23.1-33.6 Mbp. However, this analyses extends the most distal end of the DBA/2J region: the most distal markers tested were DBA/2J allele-type (82.2, 85.1, 93.0 and 94.0 Mbp). Additionally, three markers within this region were C57BL/6J allele-type (at 65.3, 65.9 and 66.8 Mbp), flanked by DBA/2J allele-type markers proximally at 61.9 Mbp and distally at 77.8 Mbp. Taken together, this suggests these mice have two DBA/2J congenic regions on chromosome 13: one spanning at least from 33.6-61.9 Mbp and the other spanning at least from 77.8-94.0 Mbp.

Development

This congenic panel was initiated by a cross of B6D2F1/J females (Stock No. 100006) to C57BL/6J males (Stock No. 000664).

Male progeny at each generation were selected by marker assisted genome scan and were backcrossed to C57BL/6J females to produce the N3-N6 generations. All animals in the panel carry a C57BL/6J Y chromosome. Sibling pairs at the N6 generation were intercrossed to produce homozygotes. A more detailed explanation of the chromosome intervals are described in Iakoubova et al. 2001 Genomics 74:89 and Davis et al. 2005 Genomics 86:259. Dr. Lusis donated the panel to The Jackson Laboratory in 2004.

In 2018, a 163 SNP (single nucleotide polymorphism) panel analysis distinguishing between C57BL/6J and DBA/2J was performed on this mouse line. Consistent with previous results, the DBA/2J congenic region on chromosome 13 showed the proximal crossover point between 23.1-33.6 Mbp. However, this analyses extends the most distal end of the DBA/2J region: the most distal markers tested were DBA/2J allele-type (82.2, 85.1, 93.0 and 94.0 Mbp). Additionally, three markers within this region were C57BL/6J allele-type (at 65.3, 65.9 and 66.8 Mbp), flanked by DBA/2J allele-type markers proximally at 61.9 Mbp and distally at 77.8 Mbp. Taken together, this suggests these mice have two DBA/2J congenic regions on chromosome 13: one spanning at least from 33.6-61.9 Mbp and the other spanning at least from 77.8-94.0 Mbp.

Allele

MGI accession ID: MGI:105837
Marker symbol: D13Mit314
Marker name: DNA Segment, Chr 13 Massachusetts Institute of Technology 314
Chromosome: 13

Allele

MGI accession ID: MGI:89741
Marker symbol: D13Mit198
Marker name: DNA segment, Chr 13, Massachusetts Institute of Technology 198
Chromosome: 13