C57BL/6-Tg(CAG-OVAL)916Jen/J

Stock number: 005145
Product name: Act-mOVA
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Ovalbumin expression in mice of this strain is detected immunohistochemically on the surfaces of cells of all organs and functions in skin-graft experiments as a minor histocompatibility antigen that triggers graft rejection by a mechanism involving both CD4 and CD8 T lymphocytes. This mutant mouse strain represents a model that may be useful in studies of adoptive transfer and graft rejection as source for tissue and cells expressing a defined minor histocompatibility antigen.

Detailed description

Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the membrane bound chicken ovalbumin OVA gene under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer. Chicken ovalbumin expression is detected by immunohistochemical analysis of all tissues. Splenocytes from transgenic mice display the 254-267-K^b complex, which is recognized by T-cells from the transgenic strain C57BL/6-Tg(TcraTcrb)1100Mjb/J (Stock No. 3831) and the 323-339-I-A^b complex, which is recognized by T-cells from the transgenic C57BL/6-Tg(TcraTcrb)425Cbn/J (Stock No. 4194). Skin grafts from transgenic mice are rejected by C57BL/6 recipients. Ovalbumin antigen specific T cells can be tracked in vivo. This mutant mouse strain represents a model that may be useful in studies of adoptive transfer and graft rejection as source for tissue and cells expressing a defined minor histocompatibility antigen.

Development

A transgenic construct containing sequence encoding chicken ovalbumin, the leader of the H-2K^b and the transmembrane region of the H-2D^b gene under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer, and a rabbit beta-globin poly(A) signal, was introduced into C57BL/6 donor eggs. The resulting male transgenic mouse (916, also referred to as Act-mOVA-II) was crossed to C57BL/6 female mice.

A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 5 markers that determine C57BL/6J from C57BL/6N was found to be segregating, the rd8 recessive mutation associated with retinal degeneration on Chromosome 1 within the Crb1 locus. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6N;C57BL/6J genetic background.

Allele

Symbol: Tg(CAG-OVAL)916Jen
Name: transgene insertion 916, Marc K Jenkins
MGI accession ID: MGI:3050829
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(CAG-OVAL)916Jen
Marker name: transgene insertion 916, Marc K Jenkins
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: OVA,ovalbumin,chicken
Molecular note: The transgene consists of a full-length chicken ovalbumin cDNA preceded by the 5' leader sequence of the mouse H2-K^b gene and followed by the DNA sequence encoding the short extracellular spacer and transmembrane domain of the mouse H2-D^b gene joined to the rabbit beta-globin polyadenylation signal; its expression is driven by the chicken beta-actin promoter under control of the CMV immediate early enhancer. Ovalbumin expression was detected immunohistochemically on the surfaces of cells of all organs and shown to function in skin-graft experiments as a minor histocompatibility antigen that triggered graft rejection by a mechanism involving both CD4 and CD8 T lymphocytes.