Ovalbumin expression in mice of this strain is detected immunohistochemically on the surfaces of cells of all organs and functions in skin-graft experiments as a minor histocompatibility antigen that triggers graft rejection by a mechanism involving both CD4 and CD8 T lymphocytes. This mutant mouse strain represents a model that may be useful in studies of adoptive transfer and graft rejection as source for tissue and cells expressing a defined minor histocompatibility antigen.
Marc Jenkins, University of Minnesota
Genetic Background | Generation |
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N?+N30
|
Allele Type |
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Transgenic (Inserted expressed sequence) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the membrane bound chicken ovalbumin OVA gene under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer. Chicken ovalbumin expression is detected by immunohistochemical analysis of all tissues. Splenocytes from transgenic mice display the 254-267-Kb complex, which is recognized by T-cells from the transgenic strain C57BL/6-Tg(TcraTcrb)1100Mjb/J (Stock No. 3831) and the 323-339-I-Ab complex, which is recognized by T-cells from the transgenic C57BL/6-Tg(TcraTcrb)425Cbn/J (Stock No. 4194). Skin grafts from transgenic mice are rejected by C57BL/6 recipients. Ovalbumin antigen specific T cells can be tracked in vivo. This mutant mouse strain represents a model that may be useful in studies of adoptive transfer and graft rejection as source for tissue and cells expressing a defined minor histocompatibility antigen.
A transgenic construct containing sequence encoding chicken ovalbumin, the leader of the H-2Kb and the transmembrane region of the H-2Db gene under the control of a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer, and a rabbit beta-globin poly(A) signal, was introduced into C57BL/6 donor eggs. The resulting male transgenic mouse (916, also referred to as Act-mOVA-II) was crossed to C57BL/6 female mice.
Expressed Gene | OVA, ovalbumin, chicken |
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Site of Expression |
Allele Name | transgene insertion 916, Marc K Jenkins |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Actin-mOVA; actin-OVA; ActmOVA; Act-mOVA; actOVA; Tg(ACTB-OVA)916Jen; Tg(CAG-OVA)916Jen |
Gene Symbol and Name | Tg(CAG-OVAL)916Jen, transgene insertion 916, Marc K Jenkins |
Gene Synonym(s) | |
Promoter | ACTB, actin beta, human |
Expressed Gene | OVA, ovalbumin, chicken |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The transgene consists of a full-length chicken ovalbumin cDNA preceded by the 5' leader sequence of the mouse H2-Kb gene and followed by the DNA sequence encoding the short extracellular spacer and transmembrane domain of the mouse H2-Db gene joined to the rabbit beta-globin polyadenylation signal; its expression is driven by the chicken beta-actin promoter under control of the CMV immediate early enhancer. Ovalbumin expression was detected immunohistochemically on the surfaces of cells of all organs and shown to function in skin-graft experiments as a minor histocompatibility antigen that triggered graft rejection by a mechanism involving both CD4 and CD8 T lymphocytes. |
Mutations Made By | Ben Ehst, University of Minnesota |
As preliminary attempts to make a homozygous colony at The Jackson Laboratory yielded smaller than normal homozygous males (September 2006), this strain is maintained by breeding hemizygotes to wildtype siblings or C57BL/6J mice.
When using the Act-mOVA mouse strain in a publication, please cite the originating article(s) and include JAX stock #005145 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(CAG-OVA)916Jen |
Frozen Mouse Embryo | C57BL/6-Tg(CAG-OVA)916Jen/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(CAG-OVA)916Jen/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(CAG-OVA)916Jen/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(CAG-OVA)916Jen/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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