These NOD.RIP-Cat mice express a transgene containing a rat catalase cDNA and show an increase in catalase activity.
Paul N. Epstein, University of Louisville
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Transgenic mice are viable, fertile, and normal in size, display accelerated spontaneous diabetes onset in males, and have coat color pigmentation. Northern blot analysis and immunohistochemistry confirm pancreatic islet specific expression of the Ins1-Cat transgene. There is a 50-fold increase in catalase activity in transgenic islets when compared to wild-type controls, which approximates catalase activity normally found in the liver. Insulin and DNA content, insulin staining and islet morphology in transgenic animals is essentially the same as in wild-type controls.
Transgenic islets have a similar reactive oxygen species (ROS) level as wild-type controls in normal media. However, after exposure to cytokines there is a significant increase of ROS fluorescence in control islets, but much smaller increase in transgenic islets. Transgenic mice are resistant to diabetes when treated with streptozotocin or alloxan. Transgenic beta cells generate less ROS fluorescence when treated with hydrogen peroxide, superoxide or peroxynitrite, but surprisingly cyclophosphamide accelerates diabetes onset in transgenic mice.
This model provides a tool for looking at the role of oxidative stress in diabetes.
NOD.FVB-Tg(Ins1-Cat, Tyr)25Pne/PneJ expresses the full-length rat catalase cDNA under the control of the rat insulin 1 promoter. In addition, these mice express tyrosinase under the control of the tyrosinase promoter, commonly referred to as TyBs or tyrosinase minigene (Xu et al, 1999, Overbeek et al., 1991). These transgenes were first co-inserted by Xu et al., (1999) into FVB oocytes. Transgenic founders carrying both genes were mated to FVB and found to co-segregate. This strain was maintained by Epstein et al., and backcrossed to NOD for 8 generations. A genome wide scan confirmed that all known Idd markers were homozygous for the NOD allele. In 2004, The Jackson Laboratory received NOD.FVB-Tg(Ins1-Cat,Tyr)25Pne/PneJ at generation N8F7.
Expressed Gene | Cat, catalase, rat |
---|---|
Site of Expression |
Allele Name | transgene insertion 25, Paul N Epstein |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | RipCat |
Gene Symbol and Name | Tg(Ins1-Cat,Tyr)25Pne, transgene insertion 25, Paul N Epstein |
Gene Synonym(s) | |
Promoter | Ins1, insulin 1, rat |
Expressed Gene | Cat, catalase, rat |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | The symbol represents a double transgene. In one transgene, a rat insulin I promoter drives the expression of a rat catalase cDNA. Northern blot analysis and immunohistochemistry detected expression of this transgene in pancreatic beta cells. A second transgene that expresses tyrosinase under control of the tyrosinase promoter was coinjected with the first transgene, and apparently cosegregated. |
Mutations Made By | Paul Epstein, University of Louisville |
When using the NOD.RIP-Cat mouse strain in a publication, please cite the originating article(s) and include JAX stock #005114 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Ins1-Cat,Tyr)25Pne |
Frozen Mouse Embryo | NOD.FVB-Tg(Ins1-Cat;Tyr)25Pne/PneJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.FVB-Tg(Ins1-Cat;Tyr)25Pne/PneJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.FVB-Tg(Ins1-Cat;Tyr)25Pne/PneJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | NOD.FVB-Tg(Ins1-Cat;Tyr)25Pne/PneJ Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.