| Allele Name | transgene insertion 2, Constance L Cepko |
| Allele Type | Transgenic (Recombinase-expressing, Reporter) |
| Allele Synonym(s) | Chx10 BAC; Chx10 BAC line 2; Chx10::Cre; Chx10CreGFP; Chx10-cre; Tg(Chx10-EGFP/cre-ALPP)2Clc; Tg(Chx10-GFP/cre, ALPP)2Clc/J; Tg(Vsx2-EGFP/cre-ALPP)2Clc |
| Gene Symbol and Name | Tg(Chx10-EGFP/cre,-ALPP)2Clc, transgene insertion 2, Constance L Cepko |
| Gene Synonym(s) | |
| Promoter | Vsx2, visual system homeobox 2, rat |
|---|
| Expressed Gene | ALPP, alkaline phosphatase, placental, human |
|---|
| Expressed Gene | GFP, Green Fluorescent Protein, |
|---|
| Expressed Gene | cre, cre recombinase, bacteriophage P1 |
|---|
| Site of Expression | expression pattern is mosaic, but specific to the retina and Muller glial cells |
|---|
| Strain of Origin | C57BL/6 and SJL |
| Chromosome | UN |
| General Note | According to Rowan and Cepko (2004), the modified BAC DNA "was injected into male pronuclei of SJL/B6 fertilized eggs." Two transgenic lines were generated. Line 1 exhibited highly mosaic expression of the EGFP reporter. Line 2 expressed EGFP more uniformly, but weakly; excision of the FRT-neo cassette increased EGFP expression in subsequent generations, so line 2 was used for further studies. Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Alkaline phosphatase expression is mosaic, but specific to the retina, and is also detected in Muller glial cells. Green Fluorescent Protein (GFP) expression is detected in the outer neuroblastic layer of the retina at embryonic days 14.5, 17.5 and neonates. When crossed to a cre reporter strain, the resulting mice exhibit mosaicism in reporter gene expression. |
| Molecular Note | This reporter gene expresses an enhanced green fluorescent protein-Cre recombinase fusion protein and alkaline phosphatase under control of Chx10 promoter and enhancer elements.The transgene was generated in a 129/Sv-derived bacterial artificial chromosome (BAC) containing the Chx10 gene and extending approximately 55 kb upstream of the translation start site and about 22 kb downstream of the polyadenylation signal. The 8th codon of Chx10 is joined in-frame to an EGFP-cre fusion gene followed by an internal ribosome entry sequence (IRES) coupled to the human placental alkaline phosphatase gene; 32 codons are deleted from within the N-terminal coding sequence of Chx10. The transgene was found by Southern blot analysis to be present in multiple copies, most or all of them intact. The mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice to remove the FRT-flanked selection cassette. |
