NOD/ShiLtDvs

Stock number: 005100
Strain synonyms: NOD/LtDvs
Research areas: Immunology, Inflammation and Autoimmunity Research, Neurobiology Research, Research Tools, Sensorineural Research

Description

This NOD subline, which separated from NOD/ShiLtJ in 1992, has the same diabetes phenotype as NOD/ShiLtJ with similar onset. However, in addition to many SNP variants this strain also has an intragenic deletion of Icam2, which results in a null allele. No impact on the diabetes phenotype has been attributed to this loss of Icam2.

Development

The NOD/ShiLtDvs inbred substrain separated from NOD/ShiLt in 1992, before that strain reached generation F70. It was maintained by sibling inbreeding in the laboratory of Dr. David Serreze and embryos were cryopreserved in 2007 at generation +F39. The 111,160 bp deletion on Chromozome 3 was present in NOD/ShiLt before the separation of this substrain and the spontaneous Icam2^m1Dvs null mutation that is specific to this substrain arose in approximately the year 2000.

Allele

Symbol: mt-Tr^m2
Name: mutation 2
MGI accession ID: MGI:3832223
Generation method: Spontaneous
Marker symbol: mt-Tr
Marker name: mitochondrially encoded tRNA arginine
Chromosome: MT
Strain of origin: various
Molecular note: The adenine repeat in the D stem is polymorphic with 9 adenines in this allele.
General note: This polymorphism is present in ALR/Lt, NOD/ShiLtDvs, and SKH2/J. A variant with 10 adenines is found in A/J, ALS/Lt, NOD/ShiLtJ and NZB/B1NJ.

Allele

Symbol: Il2^m1
Name: mutation 1
MGI accession ID: MGI:4352594
Generation method: Spontaneous
Marker symbol: Il2
Marker name: interleukin 2
Chromosome: 3
Strain of origin: multiple strains
Molecular note: This hypoactive polymorphism, found in MRL/MpJ, SJL/J, and NOD/ShiLtJ inbred strains, includes a smaller polyglutamine tract predicted to shorten the first alpha helix, which forms part of the IL2 receptor binding site.

Allele

Symbol: Gpr84^del
Name: deletion
MGI accession ID: MGI:5812913
Generation method: Spontaneous
Attributes: Null/Knockout
Marker symbol: Gpr84
Marker name: G protein-coupled receptor 84
Chromosome: 15
Strain of origin: multiple strains
Molecular note: This spontaneously arising frameshift deletion is located in exon 2 at position 103308576 bp (NCBI Build 37) and results in a premature stop codon. The mutation is predicted to result in a truncated protein lacking the transmembrane domains 4-7. The inbred strains BDP/J, DBA/1J, DBA/2J, I/LnJ, FVB/NJ, LG/J, MRL/MpJ, NODShi/LtJ, NOR/LtJ, P/J, PL/J, SKHIN/Sprd, SJL/J, SM/J are homozygous for the deletion. The allele is segregating in the outbred stocks ICR and CD-1.

Allele

Symbol: Hc⁰
Name: deficient
MGI accession ID: MGI:3027637
Generation method: Spontaneous
Marker symbol: Hc
Marker name: hemolytic complement
Chromosome: 2
Strain of origin: multiple strains
Molecular note: A 2 base "TA" deletion at positions 62 and 63 of an 83 base pair exon near the 5' end of the gene is found in the following mouse strains: A/HeJ, A/J, AKR/J, DBA/2J, I/LnJ, KK/HlJ, MOLF/EiJ, NZB/B1NJ, RF/J, ST/bJ SWR/J, B10.D2/oSnJ. The consequence of this deletion is the creation of a stop codon starting four bases after the deletion. A truncated product of 216 amino acids is predicted as a result although contradictory reports exist that a larger pro-C5 protein may be synthesized. Nevertheless, macrophages from mouse strains carrying this allele do not secrete complement 5.
General note:

This is an allele characteristic of various inbred mouse strains including the following: A/HeJ, A/J, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ

Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The A/J allele contains a 2 bp deletion resulting in deficient Hc mRNA and protein production and is associated with susceptibility to allergen-induced bronchial hyperresponsiveness. (J:108211)

Allele

Symbol: H2^g7
Name: g7 variant
MGI accession ID: MGI:3579321
Generation method: Not Applicable
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Chromosome: 17
Strain of origin: Not Applicable
General note: The g7 variant has been observed in the following strains: DBR7, NON.NOD-H2^g7

Allele

Symbol: Cdh23^ahl
Name: age related hearing loss 1
MGI accession ID: MGI:3028349
Generation method: Spontaneous
Marker symbol: Cdh23
Marker name: cadherin 23 (otocadherin)
Chromosome: 10
Strain of origin: multiple strains
Molecular note: Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further analysis has shown this is caused by a G to A transition at coding nucleotide position 753 of Cdh23 (SNP rs257098870). This hypomorphic allele changes splice donor site G-GT to A-GT, causing frame skipping of exon 7. This is predicted to delete part of the 2nd and 3rd ectodomains and cause reduced message stability. Twenty-seven strains classified with ahl and carrying the 753A allele include: CD-1, RBF/DnJ, PL/J, AKR/J, RF/J, BALB/cBy, A/WySnJ, P/J, SENCARA/PtJ, DBA/1J, ALS/LtJ, C58/J, C57BLKS/J, 129P1/ReJ, C57BR/cd, SKH2/J, BUB/Bn, MA/MyJ, LP/J, 129X1/SvJ, NOR/LtJ, A/J, C57BL/6, NOD/LtJ, DBA/2J, ALR/LtJ, C57L/J. Strains classified with ahl that DO NOT carry this mutation include: 129S1/SvImJ, C3H/HeSnJ, I/LnJ, YBR/Ei, MRL/MpJ.

Allele

Symbol: Del(3)1Lt
Name: deletion, Chr 3, Edward H Leiter 1
MGI accession ID: MGI:6120887
Generation method: Spontaneous
Attributes: Null/Knockout
Marker symbol: Del(3)1Lt
Marker name: deletion, Chr 3, Edward H Leiter 1
Chromosome: 3
Strain of origin: NOD/ShiLtJ
Molecular note: This spontaneous 111,169 bp deletion spans from Chr 3: 24,170,854 bp to 24,282,022 bp (GRCm38/mm10), and includes several QTL and the gene N-acetylated alpha-linked acidic dipeptidase-like 2

Allele

Symbol: Icam2^m1Dvs
Name: mutation 1, David V Serreze
MGI accession ID: MGI:6120486
Generation method: Spontaneous
Attributes: Null/Knockout
Marker symbol: Icam2
Marker name: intercellular adhesion molecule 2
Chromosome: 11
Strain of origin: NOD/ShiLtDvs
Molecular note: Exome sequencing shows this spontaneous intragenic deletion removes all exons except the last one (ENSMUSE00000374954) and this mutation is believed to have arisen in the NOD/ShiLtDvs inbred substrain in approximately the year 2000.