These mice carry an ENU-induced mutation characterized by retinal vasculopathy.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (ENU) | Lama1 | laminin, alpha 1 |
Homozygotes have retinal vasculopathy, characterized by vitreal fibroplasias and vessel tortuosity. There is thinning of the peripheral inner nuclear layer and variable cell loss in the retinal ganglion cell layer, along with reduced dark and light adapted electroretinogram amplitudes. There is abnormal migration of retinal astrocytes into the vitreous and the persistence of hyaloid vaculature. While targeted disruption of the alpha 1 laminin gene causes a more severe phenotype, including the absence of the inner limiting membrane, this hypomorph nevertheless displays ectopic cells and blood vessels within the vitreous indicative of reduced integrity of the inner limiting membrane.
A C57BL/6J male treated with N-ethyl-N-nitrosourea was bred with a C57BL/6J female, a resulting F1 male was backcrossed to C57BL/6J females from which an N2 female offspring was backcrossed to the F1 male parent to generate a population expressing the captured recessive mutations. From this population nmf223 was identified and kept on the C57BL/6J background. In 2004 embryos were generated for cryopreservation via in vitro fertilization using C57BL/6J females and a homozygous male at generation N3F1.
Allele Name | neuroscience mutagenesis facility, 223 |
---|---|
Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | |
Gene Symbol and Name | Lama1, laminin, alpha 1 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 17 |
Molecular Note | An A to G missense mutation results in a tyrosine to cysteine mutation at the highly conserved amino acid 265, which lies in the end of the N-terminal domain. |
When using the nmf223 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005094 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
unverified Heterozygous for Lama1<nmf223> |
Frozen Mouse Embryo | C57BL/6J-Lama1<nmf223>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Lama1<nmf223>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Lama1<nmf223>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Lama1<nmf223>/J Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.