These mice carry an ENU-induced mutation characterized by body tremor, spasms, and abnormal hind leg movement.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | Cacna2d2 | calcium channel, voltage-dependent, alpha 2/delta subunit 2 |
The mutants can be detected at or shortly after weaning age (average age of onset 5 weeks +/-1.9; n=9). They are small, show body tremor, splayed hind limbs, and might advance with both hind limbs simultaneously, in a 'frog-like' manner. Intermittently, body spasms can be observed, which may be limited to one or the other hind- or fore limb, or to the head, neck, shoulder and front limb area, or include the whole body, so the animal needs to lie on its side. Either episode may last for several seconds. Because of the observed body spasms and splayed hind limbs, allele tests were performed with Cacna2d2 (calcium channel, voltage-dependent, alpha 2/delta subunit 2) or Cacnb4 (calcium channel, voltage-dependent, beta 4 subunit) mutants. The results showed NMF299 to be an allele of Cacna2d2, i.e. 2 heterozygous matings (w/ducky-2J) resulted in 2 affected mice in a total of 12 progeny. NMF299 are now backcrossed to the BALB/cByJ background. Standard pathology work-up on one mutant (57 days of age) showed no abnormalities, except body and organs to be small and immature.
With special care and feeding mutants can live to over 2 months of age, without it, they may die as early as 4 weeks of age. Males are fertile, females may require ovarian transplants.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. This line was back-crossed to BALB/cByJ beyond the N5 generation.
Allele Name | ducky 3 Jackson |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | Cacna2d2nmf299; neuroscience mutagenesis facility, 299; NMF299 |
Gene Symbol and Name | Cacna2d2, calcium channel, voltage-dependent, alpha 2/delta subunit 2 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 9 |
Molecular Note | This phenotypic mutation, identified in an ENU mutagenesis screen, was shown by complementation testing to be an allele of Cacna2d2. |
With special care and feeding mutants can live to over 2 months of age, without it, they may die as early as 4 weeks of age. Males are fertile, females may require ovarian transplants.
When using the ducky 3 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #005050 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Unknown for Cacna2d2<du-3J> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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