| Allele Name | transgene insertion 1, Thomas M Jessell |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | Gfp-HB9; Hb9::EGFP; Hb9:GFP-1B; Hb9-eGFP; Hb9-Gfp; Hlxb9:GFP |
| Gene Symbol and Name | Tg(Hlxb9-GFP)1Tmj, transgene insertion 1, Thomas M Jessell |
| Gene Synonym(s) | |
| Promoter | Mnx1, motor neuron and pancreas homeobox 1, mouse, laboratory |
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| Expressed Gene | GFP, Green Fluorescent Protein, |
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| Site of Expression | Dendrites, axons, and soma of spinal motor neurons display distinct expression of GFP. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites, and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. |
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| Strain of Origin | (C57BL/6 x CBA)F1 |
| Chromosome | 15 |
| General Note | Homozygous transgenic mice on a genetic background that involves C57BL/6 and CBA are viable and fertile. They do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons, and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. |
| Molecular Note | The transgenic construct contains a 9kb sequence of the 5' portion of the mouse Hlxb9 (Mnx1) gene, a 5' splice substrate, an enhanced Green Fluorescent Protein (EGFP) open reading frame, and a bovine growth hormome polyadenylation site sequence. Line 1 contains 10 to 11 tandem copies of the transgene. The expression pattern of this transgene is the same as that for the endogenous gene from which the promoter is derived. |
| Mutations Made By | Ivo Lieberam, Columbia University/HHMI |
