B6.Cg-Tg(Hlxb9-GFP)1Tmj/J

Stock number: 005029
Product name: Hb9:GFP
Research areas: Neurobiology Research, Research Tools

Description

Mice homozygous for the transgene display distinct expression of GFP in dendrites, axons and soma of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. Expression mimics endogenous HLXB9 expression pattern. This mutant mouse strain represents a model that may be useful for purification and in vivo tracking of spinal motor neurons.

Detailed description

These transgenic mice express Green Fluorescent Protein (GFP) under the direction of the mouse Mnx1 (also called Hlxb9) promoter. Mice homozygous for the transgenic insert are viable, fertile, do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. This mutant mouse strain represents a model that may be useful for purification and in vivo tracking of spinal motor neurons.

Development

A transgenic construct containing a 9kb sequence of the 5' portion of the mouse Mnx1 gene, a GFP open reading frame, and bovine growth hormome polyadenylation site sequence was injected into fertilized B6CBAF1 mouse eggs.

Allele

Symbol: Tg(Hlxb9-GFP)1Tmj
Name: transgene insertion 1, Thomas M Jessell
MGI accession ID: MGI:3056906
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Hlxb9-GFP)1Tmj
Marker name: transgene insertion 1, Thomas M Jessell
Chromosome: 15
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Dendrites, axons, and soma of spinal motor neurons display distinct expression of GFP. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites, and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice.
Molecular note: The transgenic construct contains a 9kb sequence of the 5' portion of the mouse Hlxb9 (Mnx1) gene, a 5' splice substrate, an enhanced Green Fluorescent Protein (EGFP) open reading frame, and a bovine growth hormome polyadenylation site sequence. Line 1 contains 10 to 11 tandem copies of the transgene. The expression pattern of this transgene is the same as that for the endogenous gene from which the promoter is derived.
General note: Homozygous transgenic mice on a genetic background that involves C57BL/6 and CBA are viable and fertile. They do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons, and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice.