Mice homozygous for the transgene display distinct expression of GFP in dendrites, axons and soma of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. Expression mimics endogenous HLXB9 expression pattern. This mutant mouse strain represents a model that may be useful for purification and in vivo tracking of spinal motor neurons.
Thomas M. Jessell, Columbia University/HHMI
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These transgenic mice express Green Fluorescent Protein (GFP) under the direction of the mouse Mnx1 (also called Hlxb9) promoter. Mice homozygous for the transgenic insert are viable, fertile, do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. This mutant mouse strain represents a model that may be useful for purification and in vivo tracking of spinal motor neurons.
A transgenic construct containing a 9kb sequence of the 5' portion of the mouse Mnx1 gene, a GFP open reading frame, and bovine growth hormome polyadenylation site sequence was injected into fertilized B6CBAF1 mouse eggs.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Dendrites, axons, and soma of spinal motor neurons display distinct expression of GFP. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites, and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. |
Allele Name | transgene insertion 1, Thomas M Jessell |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Gfp-HB9; Hb9::EGFP; Hb9:GFP-1B; Hb9-eGFP; Hb9-Gfp; Hlxb9:GFP |
Gene Symbol and Name | Tg(Hlxb9-GFP)1Tmj, transgene insertion 1, Thomas M Jessell |
Gene Synonym(s) | |
Promoter | Mnx1, motor neuron and pancreas homeobox 1, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Dendrites, axons, and soma of spinal motor neurons display distinct expression of GFP. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites, and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | 15 |
General Note | Homozygous transgenic mice on a genetic background that involves C57BL/6 and CBA are viable and fertile. They do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons, and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. |
Molecular Note | The transgenic construct contains a 9kb sequence of the 5' portion of the mouse Hlxb9 (Mnx1) gene, a 5' splice substrate, an enhanced Green Fluorescent Protein (EGFP) open reading frame, and a bovine growth hormome polyadenylation site sequence. Line 1 contains 10 to 11 tandem copies of the transgene. The expression pattern of this transgene is the same as that for the endogenous gene from which the promoter is derived. |
Mutations Made By | Ivo Lieberam, Columbia University/HHMI |
The resulting male transgenic mice were backcrossed for 8 generations on the C57BL/6J background. The strain is maintained as a hemizygote, due to high mortality of homozygous pups born to homozygous females.
When using the Hb9:GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #005029 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Hlxb9-GFP)1Tmj |
Frozen Mouse Embryo | B6.Cg-Tg(Hlxb9-GFP)1Tmj/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Hlxb9-GFP)1Tmj/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Hlxb9-GFP)1Tmj/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Hlxb9-GFP)1Tmj/J Frozen Embryos | $3373.50 |
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