FVB-Tg(ZP2)2Dean/J

Stock number: 005021
Product name: huZP2
Research areas: Developmental Biology Research, Reproductive Biology Research

Description

These huZP2 mice express the human zona pellucida 2 (ZP2) protein that rescues the homozygous null Zp2 infertility phenotype.

Detailed description

These transgenic mice express the human zona pellucida 2 (ZP2) protein. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes die shortly after birth. Transgene expression (mRNA and protein) is oocyte specific. This transgene rescues the homozygous null Zp2 infertility phenotype (see Stock No. 004129). This mutant mouse strain represents a model that may be useful in studies of reproduction and fertility.

Development

A transgenic construct containing 16.3 Kb sequence containing the entire coding region of the human ZP2 gene and 2.3 Kb of 5' flanking sequence was injected into fertilized FVB/N mouse eggs.

Allele

Symbol: Tg(ZP2)2Dean
Name: transgene insertion 2, Jurrien Dean
MGI accession ID: MGI:3057275
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(ZP2)2Dean
Marker name: transgene insertion 2, Jurrien Dean
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: ZP2,zona pellucida glycoprotein 2,human
Molecular note: The transgene consists of a 16.3 kb human genomic DNA fragment containing all 19 exons of the zona pellucida 2 gene and 2.3 kb of 5' flanking DNA. Northern blot analysis and in situ hybridization of ovarian tissue demonstrated oocyte-specific transgene expression. Immunoblot analysis revealed coexpression of mouse and human ZP2 in oocytes. The molecular mass of the transgenic protein suggests it undergoes post-translational modification similarly to the native human protein.
General note: 3 transgenic lines, designated by the authors TgN(HuZP2)1NIH-3NIH, were generated. All three lines were used in experiments, and no distinction was made among their phenotypes.