These huZP2 mice express the human zona pellucida 2 (ZP2) protein that rescues the homozygous null Zp2 infertility phenotype.
Jurrien Dean, NIH / NIDDK
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These transgenic mice express the human zona pellucida 2 (ZP2) protein. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes die shortly after birth. Transgene expression (mRNA and protein) is oocyte specific. This transgene rescues the homozygous null Zp2 infertility phenotype (see Stock No. 004129). This mutant mouse strain represents a model that may be useful in studies of reproduction and fertility.
A transgenic construct containing 16.3 Kb sequence containing the entire coding region of the human ZP2 gene and 2.3 Kb of 5' flanking sequence was injected into fertilized FVB/N mouse eggs.
Expressed Gene | ZP2, zona pellucida glycoprotein 2, human |
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Site of Expression |
Allele Name | transgene insertion 2, Jurrien Dean |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | huZP2; TgN(huZP2)2Dean; TgN(HuZP2)2NIH |
Gene Symbol and Name | Tg(ZP2)2Dean, transgene insertion 2, Jurrien Dean |
Gene Synonym(s) | |
Promoter | ZP2, zona pellucida glycoprotein 2, human |
Expressed Gene | ZP2, zona pellucida glycoprotein 2, human |
Strain of Origin | FVB/N |
Chromosome | UN |
General Note | 3 transgenic lines, designated by the authors TgN(HuZP2)1NIH-3NIH, were generated. All three lines were used in experiments, and no distinction was made among their phenotypes. |
Molecular Note | The transgene consists of a 16.3 kb human genomic DNA fragment containing all 19 exons of the zona pellucida 2 gene and 2.3 kb of 5' flanking DNA. Northern blot analysis and in situ hybridization of ovarian tissue demonstrated oocyte-specific transgene expression. Immunoblot analysis revealed coexpression of mouse and human ZP2 in oocytes. The molecular mass of the transgenic protein suggests it undergoes post-translational modification similarly to the native human protein. |
Mutations Made By | Jurrien Dean, NIH / NIDDK |
A transgenic construct containing 16.3 Kb sequence containing all 19 exons of the human ZP2 gene and 2.3 Kb of 5' flanking sequence was used to create transgenic animals on the FVB/N background. Homozygous mice are perinatal lethal.
When using the huZP2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005021 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(ZP2)2Dean |
Frozen Mouse Embryo | FVB-Tg(ZP2)2Dean/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(ZP2)2Dean/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(ZP2)2Dean/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(ZP2)2Dean/J Frozen Embryos | $3373.50 |
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