These mutants were detected through routine inspections for overt abnormal behavior and the onset of head- and body tilt has been determined to be at 4 weeks of age (+/- 0.3 weeks); occasional circling behavior has also been observed. Results of Auditory brainstem recordings (n=2) were normal. Complementation analysis, i.e. matings between heterozygous nmf250 (female) and homozygous Nox3 mice (male, Stock Number 002557, B6Ei.GL-Nox3^het/J) resulted in 5 mutants in a total of 7 progeny, demonstrating that nmf250 represents an allele of Nox3. Standard pathology work-up on two mutants (43 or 112 days of age) revealed no abnormalities. Serial sections of the ears of the older mouse showed a lack of otoconia in the ear, which was confirmed by examination of ear whole mounts of two other mutants. No other abnormalities were observed. Male or female mutants have been produced and the colony can usually be maintained through homozygous matings.

Development

This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Nox3^het-4J

Allele Symbol: Nox3^het-4J

Allele Name	head tilt 4 Jackson
Allele Type	Chemically induced (ENU)
Allele Synonym(s)	neuroscience mutagenesis facility, 250; nmf250; Nox3^nmf250
Gene Symbol and Name	Nox3, NADPH oxidase 3
Gene Synonym(s)
Strain of Origin	C57BL/6J
Chromosome	17
Molecular Note	This phenotypic mutation, discovered in an ENU mutagenesis screen, was shown to be an allele of Nox3 by its failure to complement the original het mutation. A T-to-A transversion has been identified in the splice donor consensus sequence at the 3' end of exon 10.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Nox3^het-4J related

Neurobiology Research
- Neuroscience Mutagenesis Facility Strain
Sensorineural Research
- Vestibular Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Nox3^het-4J/Nox3^het-4J
C57BL/6J-Nox3/J

behavior/neurological phenotype

circling
- mutant mice begin to exhibit circling behavior at 4 +/- 0.3 weeks of age

head tilt
- mutant mice begin to exhibit head tilting at 4 +/- 0.3 weeks of age

mammalian phenotype

hearing/vestibular/ear phenotype
- Normal - auditory brainstem response (ABR) testing of two affected mice indicated their hearing was normal

References

Additional - Nox3^het-4J related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Sanger sequencing:Nox3
- Genotyping resources and troubleshooting
Appearance
- black
  Related Genotype: a/a
Citation
When using the head tilt 4 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #005014 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Nox3<het-4J>

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:C57BL/6J-Nox3nmf250/J, head tilt 4 Jackson

Genetic overview

Research Applications

Base Price

Detailed Description