These mice carry an ENU-induced mutation that is characterized by abnormal eye development.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.Read More +
Routine ophthalmoscopic examination showed hyaloid remnants and vitreal fibroplasia with white retinal spots in the majority of mutants, and cataracts, corneal scarring, microphthalmia, and/or cataracts in some mutants. The colony can be maintained through regular breeding; however, because of the low penetrance of the phenotype, which requires a large number of animals to be tested, and our limited testing capacity, we chose to cryopreserve the line. NMF131 is not available live; if you are interested in these mutants please inquire about details of availability.
Standard pathology work-up on 3 mutants (100, 116 or 173 days of age) showed variable retinal and vitreal abnormalities which include retinal dysplasia, topical degeneration and vitreal fibroplasia.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment. This mutation has been maintained on this background and in 2004 in vitro fertilization using C57BL/6J females and N4F1 homozygous males produced embryos for cryopreservation.
|Allele Name||neuroscience mutagenesis facility, 131|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||nmf131, neuroscience mutagenesis facility, 131|
|Strain of Origin||C57BL/6J|
|Molecular Note||This phenotypic mutant was identified in an ENU mutagenesis screen.|