NOD/ShiLt-Tg(Ins2-cre)3Lt/LtJ

Stock number: 004986
Product name: NOD.RIPCre3
Research areas: Diabetes and Obesity Research, Research Tools

Description

These NOD.RIPCre3 mice express cre recombinase in pancreatic beta cells.

Detailed description

This transgenic strain uses the rat insulin 2 promoter to drive expression of Cre recombinase in pancreatic beta cells. Hemizygous mice develop a diabetes that is characteristic of the NOD/ShiLt inbred strain. The investigator reports that insulin expression appears to be moderately suppressed.

Development

A transgenic construct containing the rat insulin 2 promoter and cre coding sequence was injected into fertilized NOD/ShiLt mouse eggs. The strain was developed in the laboratory of Dr. Ed Leiter at The Jackson Laboratory.

Allele

Symbol: Tg(Ins2-cre)3Lt
Name: transgene insertion 3, Edward Leiter
MGI accession ID: MGI:3721302
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Ins2-cre)3Lt
Marker name: transgene insertion 3, Edward Leiter
Chromosome: UN
Strain of origin: NOD/ShiLt
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: pancreatic beta cells
Molecular note: A construct with the rat insulin II promoter driving cre recombinase was injected into fertilized NOD/ShiLt embryos. Line 3 was established by mating to NOD/ShiLt mice prior to sibling mating. Mice overexpress Cre specifically in pancreatic beta cells.