These thymoma viral proto-oncogene 1 knock-out mice have defects in both fetal and postnatal growth persisting into adulthood, and may be useful in studies related to organismal growth.
Morris J. Birnbaum, Un of Pennsylvania Schl of Medicine
Mice that are homozygous for the targeted mutation are viable and do not display any gross behavioral abnormalities. Homozygotes exhibit lower fertility. Female homozygotes do not nurse well; up to 50% perinatal mortality can occur. No gene product (mRNA or protein) is detected by Northern or Western blot analysis of mouse embryonic fibroblasts. Homozygotes are only 80% of wildtype body weight at birth, and remain small. This mutant mouse strain may be useful in related to organismal growth.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exons 4 through 7 and the 5' end of exon 8. The construct was electroporated into 129P2Ola/Hsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were crossed to C57BL/6 mice, and then backcrossed to the same for 10 generations (March 2003).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Morris J Birnbaum|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Akt1, thymoma viral proto-oncogene 1|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A neomycin resistance gene was used to replace exons 4 through 7 and the 5' portion of exon 8. Exon 5 is known to encode an lysine residue essential for catalytic activity. Expression was undetected in homozygous mutant animals by both Northern and Western blot analyses. The authors noted that the expression of Akt2 and Akt3 was normal in mice homozygous for the targeted allele.|
|Mutations Made By|| |
Morris Birnbaum, Un of Pennsylvania Schl of Medicine
This strain originated on a B6;129 background and has been backcrossed to C57BL/6 for at least 10 generations (March 2003). The strain is maintained as a heterozygote. Homozygotes are reported to be less fertile. Female homozygotes do not nurse well; up to 50% perinatal mortality can occur.
When using the Akt1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004912 in your Materials and Methods section.