B6.129P2-Akt1^tm1Mbb/J

Stock number: 004912
Product name: Akt1 KO
Strain synonyms: Akt1/PKBα KO
Research areas: Cell Biology Research, Developmental Biology Research

Description

These thymoma viral proto-oncogene 1 knock-out mice have defects in both fetal and postnatal growth persisting into adulthood, and may be useful in studies related to organismal growth.

Detailed description

Mice that are homozygous for the targeted mutation are viable and do not display any gross behavioral abnormalities. Homozygotes exhibit lower fertility. Female homozygotes do not nurse well; up to 50% perinatal mortality can occur. No gene product (mRNA or protein) is detected by Northern or Western blot analysis of mouse embryonic fibroblasts. Homozygotes are only 80% of wildtype body weight at birth, and remain small. This mutant mouse strain may be useful in related to organismal growth.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exons 4 through 7 and the 5' end of exon 8. The construct was electroporated into 129P2Ola/Hsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were crossed to C57BL/6 mice, and then backcrossed to the same for 10 generations (March 2003).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Allele

Symbol: Akt1^tm1Mbb
Name: targeted mutation 1, Morris J Birnbaum
MGI accession ID: MGI:2180150
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Akt1
Marker name: thymoma viral proto-oncogene 1
Chromosome: 12
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin resistance gene was used to replace exons 4 through 7 and the 5' portion of exon 8. Exon 5 is known to encode an lysine residue essential for catalytic activity. Expression was undetected in homozygous mutant animals by both Northern and Western blot analyses. The authors noted that the expression of Akt2 and Akt3 was normal in mice homozygous for the targeted allele.