These mice possess loxP restriction sites on either side of the exon encoding amino acids 206-232 of the X-linked Ogt gene. Female mice bearing two copies of the floxed allele, and males carrying one, are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. At least one intact, functional Ogt allele is required for embryonic stem cell viability and subsequent ontogenic events, as well as the viability and function of germ cells, neurons, thymoyctes, fibroblasts. When used in conjunction with a Cre recombinase-expressing strain, this mutant is useful in generating tissue-specific mutants of the floxed gene.
For example, when crossed to a strain expressing Cre recombinase in thymocytes (see Stock No. 003802), this mutant mouse strain may be useful in studies of intracellular glycosylation and T cell apoptosis.
When crossed to a strain expressing Cre recombinase in embryonic neuronal cells (see Stock No. 003966), this mutant mouse strain may be useful in studies of intracellular glycosylation and tau hyperphosphorylation.
When crossed to a strain expressing Cre recombinase in the male germ line (see Stock No. 003328), this mutant mouse strain may be useful in studies of paternal X inactivation.
When crossed to a strain expressing Cre recombinase in oocytes (see Stock No. 006888), this mutant mouse strain may be useful in studies of intracellular glycosylation and embryogenesis.
