OGTF floxed mice possess loxP sites flanking the exon encoding amino acids 206-232 of the X-linked Ogt gene. These mice may be useful in studying intracellular glycosylation, lipid use by tumors, hyperpagia/obesity, metabolism, immune cells and anti-inflammatory function.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
These mice possess loxP restriction sites on either side of the exon encoding amino acids 206-232 of the X-linked Ogt gene. Female mice bearing two copies of the floxed allele, and males carrying one, are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. At least one intact, functional Ogt allele is required for embryonic stem cell viability and subsequent ontogenic events, as well as the viability and function of germ cells, neurons, thymoyctes, fibroblasts. When used in conjunction with a Cre recombinase-expressing strain, this mutant is useful in generating tissue-specific mutants of the floxed gene.
For example, when crossed to a strain expressing Cre recombinase in thymocytes (see Stock No. 003802), this mutant mouse strain may be useful in studies of intracellular glycosylation and T cell apoptosis.
When crossed to a strain expressing Cre recombinase in embryonic neuronal cells (see Stock No. 003966), this mutant mouse strain may be useful in studies of intracellular glycosylation and tau hyperphosphorylation.
When crossed to a strain expressing Cre recombinase in the male germ line (see Stock No. 003328), this mutant mouse strain may be useful in studies of paternal X inactivation.
When crossed to a strain expressing Cre recombinase in oocytes (see Stock No. 006888), this mutant mouse strain may be useful in studies of intracellular glycosylation and embryogenesis.
A targeting vector was designed to have a loxP site upstream of the Ogt exon encoding amino acids 206-232 and a loxP-flanked neo cassette just downstream of that exon. The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre-mediated recombination and no longer retained the cassette but did retain the loxP-flanked exon (also described as the OGTF allele) were injected in C57BL/6 blastocysts and chimeric mice obtained. This strain originated on a B6;129 background and has been backcrossed to C57BL/6 for at least ten generations [March 2003].
|Allele Name||targeted mutation 1, Gerald W Hart|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Ogt, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The exon encoding amino acids 206 through 232 (numbering derived from the rat cDNA encoding 1037 residues) was flanked by single loxP sites.|
|Mutations Made By|| |
Linda Walker, University of California at San Diego
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Alternatively, homozygous mice may be bred together.
When using the OGTF mouse strain in a publication, please cite the originating article(s) and include JAX stock #004860 in your Materials and Methods section.