B6.129-Ogt^tm1Gwh/J

Stock number: 004860
Product name: OGT^F
Research areas: Cell Biology Research, Research Tools

Description

OGT^F floxed mice possess loxP sites flanking the exon encoding amino acids 206-232 of the X-linked Ogt gene. These mice may be useful in studying intracellular glycosylation, lipid use by tumors, hyperpagia/obesity, metabolism, immune cells and anti-inflammatory function.

Detailed description

These mice possess loxP restriction sites on either side of the exon encoding amino acids 206-232 of the X-linked Ogt gene. Female mice bearing two copies of the floxed allele, and males carrying one, are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. At least one intact, functional Ogt allele is required for embryonic stem cell viability and subsequent ontogenic events, as well as the viability and function of germ cells, neurons, thymoyctes, fibroblasts. When used in conjunction with a Cre recombinase-expressing strain, this mutant is useful in generating tissue-specific mutants of the floxed gene.

For example, when crossed to a strain expressing Cre recombinase in thymocytes (see Stock No. 003802), this mutant mouse strain may be useful in studies of intracellular glycosylation and T cell apoptosis.

When crossed to a strain expressing Cre recombinase in embryonic neuronal cells (see Stock No. 003966), this mutant mouse strain may be useful in studies of intracellular glycosylation and tau hyperphosphorylation.

When crossed to a strain expressing Cre recombinase in the male germ line (see Stock No. 003328), this mutant mouse strain may be useful in studies of paternal X inactivation.

When crossed to a strain expressing Cre recombinase in oocytes (see Stock No. 006888), this mutant mouse strain may be useful in studies of intracellular glycosylation and embryogenesis.

Development

A targeting vector was designed to have a loxP site upstream of the Ogt exon encoding amino acids 206-232 and a loxP-flanked neo cassette just downstream of that exon. The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre-mediated recombination and no longer retained the cassette but did retain the loxP-flanked exon (also described as the OGT^F allele) were injected in C57BL/6 blastocysts and chimeric mice obtained. This strain originated on a B6;129 background and has been backcrossed to C57BL/6 for at least ten generations [March 2003].

Allele

Symbol: Ogt^tm1Gwh
Name: targeted mutation 1, Gerald W Hart
MGI accession ID: MGI:2387867
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ogt
Marker name: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Chromosome: X
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: The exon encoding amino acids 206 through 232 (numbering derived from the rat cDNA encoding 1037 residues) was flanked by single loxP sites.