These R26CreER mutant mice have a tamoxifen-inducible Cre-mediated recombination system driven by the endogenous mouse Gt(ROSA)26Sor promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions.
Of note, a similar strain (Stock No. 008463 B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J) enables temporal control of floxed gene expression in vivo and is reportedly more sensitive to tamoxifen than Stock No. 004847.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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F?+N2F14
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing, Inducible) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These R26CreER mutant mice have a tamoxifen-inducible Cre-mediated recombination system driven by the endogenous mouse Gt(ROSA)26Sor promoter. The mutant allele consists of a fusion product involving Cre recombinase and an altered version of the mouse estrogen receptor ligand binding domain. The mutant ligand binding domain does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the CRE/ESR1 protein can only gain access to the nuclear compartment to mediate recombination after exposure to tamoxifen. Tamoxifen administration will also induce Cre recombination in the developing embryos of treated mothers. When crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
A targeting vector containing a PGK-neo cassette and Cre recombinase/estrogen receptor ligand-binding domain fusion protein sequence was used to insert the fusion protein into a site located within intron 1 of the GT(ROSA)26Sor locus. The construct was introduced into 129X1/SvJ x 129S1/Sv-derived R1-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Correctly targeted ES cells were injected into C57BL/6J blastocysts to obtain chimeric animals.
Expressed Gene | cre/ER, cre inducible estrogen receptor, bacteriophage P1/Human |
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Site of Expression | tamoxifen-inducible cre; when crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions |
Allele Name | targeted mutation 1, Jeremy Nathans |
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Allele Type | Targeted (Recombinase-expressing, Inducible) |
Allele Synonym(s) | CreERT1; Gt(ROSA)26Sortm1(cre/Esr1)Nat; R26CreER; R26ERCre; R26RNatCre |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | cre/ER, cre inducible estrogen receptor, bacteriophage P1/Human |
Site of Expression | tamoxifen-inducible cre; when crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 6 |
Molecular Note | A cDNA fragment encoding a fusion protein comprised of cre recombinase and the ligand binding domain of Esr1 was inserted at the endogenous locus via homologous recombination. The initiator methionine was placed in an optimal translational context, allowing for near ubiquitous expression via the endogenous promoter. Activation of cre recombinase activity is dependent on the administration of 4-HO-tamoxifen. |
Mutations Made By | Tudor Badea, Johns Hopkins University |
This strain originated and is maintained on a B6;129 background as a homozygote.
When using the R26CreER mouse strain in a publication, please cite the originating article(s) and include JAX stock #004847 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm1(cre/Esr1)Nat> |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(cre/ERT)Nat>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(cre/ERT)Nat>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(cre/ERT)Nat>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(cre/ERT)Nat>/J Frozen Embryos | $3373.50 |
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