These mice express a transgene containing the mouse cyclin D1 gene that is over-expressed in the liver and epithelial cells of the small intestine and colon.
R. D. Beauchamp, Vanderbilt University Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These transgenic mice express the mouse cyclin D1 under the direction of the rat fatty acid binding protein 1 promoter (–596 to +21 fragment). Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Hemizygous mutant mice exhibit reduced fertility after 3 months of age. Transgenic mice overexpress cyclin D1 in the liver and epithelial cells of the small intestine and colon. A lower level of expression occurs in the kidneys. Onset of progressive liver disease begins at one month of age with hepatomegaly. Male transgenic mice exhibit more severe hepatomegaly than transgenic females. By 6 months of age, all transgenic males and half of the transgenic females display hepatocellular dysplasia. 87% of transgenic males and 69% of transgenic females develop hepatocellular adenoma or hepatocellular carcinoma by 15 months of age. By 6 months of age, approximately half of mutant mice have lost hepatic expression of Smad4. This mutant mouse strain may be useful in studies of hepatocellular carcinogenesis.
A transgenic construct containing a mouse cyclin D1 gene inserted into sequence encoding the rabbit Beta- globin under the control of the rat fatty acid binding protein 1 promoter was injected into fertilized C57BL/6 mouse eggs.
Expressed Gene | Ccnd1, cyclin D1, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 4, R Daniel Beauchamp |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Fabp1-Ccnd1)4Rdb, transgene insertion 4, R Daniel Beauchamp |
Gene Synonym(s) | |
Promoter | Fabp1, fatty acid binding protein 1, rat |
Expressed Gene | Ccnd1, cyclin D1, mouse, laboratory |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | This transgenic construct contains the mouse cyclin D1 gene driven by the rat fatty acid binding protein promoter. Transgenic mice overexpress cyclin D1 in the liver and epithelial cells of the small intestine and colon. A lower level of expression occurs in the kidneys. This line carries 20-fold more transgene copies than the F2 line, which itself carries multiple transgene copies. |
Mutations Made By | Natasha Deane, Vanderbilt University Medical Center |
This strain originated on a C57BL/6 background. When maintaining a live colony, these mice are bred as hemizygotes.
When using the F4 mouse strain in a publication, please cite the originating article(s) and include JAX stock #004834 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Fabp1-Ccnd1)4Rdb |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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