These mice carry an ENU-induced mutation characterized by circling, head tossing behavior, and deafness.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | Cdh23 | cadherin 23 (otocadherin) |
The mutants exhibit circling and head tossing behavior by weaning age (at 3-4 weeks), and three animals tested at four weeks of age for Auditory brain stem response were deaf. Mutants of either gender have been produced and the colony is maintained through regular breeding. Serial sections of the ears of two mutants (24 and 29 days of age) and additional whole ear histology on one mutant (33 days of age) revealed no abnormalities. Standard pathology work-up on all three animals showed no other abnormalities. Because of the map position of this mutation, and its phenotypic similarity to the waltzer mutations, a series of complementation tests has been performed to determine if allelic relationships exist between NMF181, NMF112, NMF252 and Cdh23. The results showed NMF181 to be an allele of NMF112(one mating produced 2 mutants in a total of 7 progeny), of NMF252(one mating produced 2 mutants in a total of 8 progeny) and therefore of Cdh23, since NMF252 has been identified as a new allele of Cdh23. This information suggests therefore that NMF181 mutants might be useful for studying neurobiological mechanisms related to Usher syndrome and human deafness.
Serial sections of the ears of two mutants (24 and 29 days of age) and additional whole ear histology on one mutant (33 days of age) revealed no abnormalities. Standard pathology work-up on all three animals showed no other abnormalities.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
Allele Name | waltzer 9 Jackson |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | Cdh23nmf181; neuroscience mutagenesis facility, 181; NMF181 |
Gene Symbol and Name | Cdh23, cadherin 23 (otocadherin) |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 10 |
Molecular Note | The mutant nmf181 has a similar phenotype and mapping position to the waltzer mutant. Complementation testing between nmf181 and a known allele at the waltzer locus, Cdh23nmf112, confirmed the allelic relationship. |
When using the waltzer 9 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #004819 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Unknown for Cdh23<v-9J> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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