These mice carry an ENU-induced mutation characterized by limb spasms.
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The hind- and front limbs of these mutants exhibit spasms intermittently, i.e. paws or the whole front limb will be contracted, or a hind leg will be briefly stretched to the side or backward while the animal moves around. These episodes are usually very brief, and can be observed at 4 weeks of age (average onset 4.6 +/- 0.8 weeks of age; n=15). The mice also rise frequently on their hind limbs. Mutants breed, and the colony is maintained through mutant x heterozygote or heterozygote x heterozygote breedings. Standard pathology work-up on two mutants (37 or 258 days of age) showed a lack of myelin in the cortical spinal tract. Bodian staining was performed on spinal cord tissue of the older mouse and revealed no other abnormalities.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||neuroscience mutagenesis facility, 161|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||nmf161, neuroscience mutagenesis facility, 161|
|Strain of Origin||C57BL/6J|
|Molecular Note||This mutation was identified in an ENU mutagenesis screen.|