STOCK Tg(KRT14-cre)1Amc/J

Stock number: 004782
Product name: K14-Cre
Research areas: Cancer Research, Developmental Biology Research, Research Tools

Description

A congenic version of this strain is available as Stock No. 018964.

The K14-Cre transgene has a human keratin 14 promoter directing expression of Cre recombinase. These K14-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in the ectoderm and its derivatives (including the skin, dental epithelium, and oral ectoderm).

Detailed description

Mice hemizygous for the K14-Cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the human keratin 14 promoter. Cre transcript is detected in the skin. When crossed to a reporter line containing Gt(ROSA)26Sor^tm1Sor, Beta-galactosidase activity is detected in the oral ectoderm at 11.75 dpc, and at 14.5 dpc activity is detected in the skin and throughout the dental epithelium. This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful to study developmentally critical gene function in the ectoderm and its derivatives.

View cre expression characterization.

Development

The K14-Cre transgene was designed with a human keratin 14 promoter/enhancer sequence, Cre recombinase gene, and the human growth hormone gene and polyadenylation signal. This transgene was introduced into B6CBAF1 donor eggs. The resulting transgenic founder animals were initially crossed to C57BL/6 mice, and then crossed to outbred Swiss Webster mice. Mice from two founder lines (199 and 200) were crossed with Shhⁿ (null allele) mice for an unknown number of times. The Shhⁿallele was subsequently bred out of the The Jackson Laboratory Repository colony before 2005.

Allele

Symbol: Tg(KRT14-cre)1Amc
Name: transgene insertion 1, Andrew P McMahon
MGI accession ID: MGI:2445832
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(KRT14-cre)1Amc
Marker name: transgene insertion 1, Andrew P McMahon
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: skin, the oral ectoderm including the dental lamina at 11.75 d.p.c., and the dental epithelium by 14.5 d.p.c.
Molecular note: The transgene is composed of a cre recombinase gene under the control of a human keratin 14 promoter. The human KRT14 promoter directs transgene expression in hair and skin. The transgene also contains a human growth hormone sequence and polyadenylation signal. When crossed to a transgenic reporter line, beta-galactosidase activity was detected in the oral ectoderm at 11.75 dpc, and at 14.5 dpc activity is detected in the skin and throughout the dental epithelium.
General note: This allele is representative of 2 founders (#199 and #200) exhibiting the highest level of transgene expression.

Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.