Overview

Also Known As: Tau KO

A congenic C57BL/6J background version of this strain is available as Stock No. 029219.

Enhanced green fluorescent protein (EGFP) is detected throughout the developing central nervous system and persists to adult, closely following the expression of neuron specific beta-tubulin III. The expression of cytoplasmic EGFP in the central nervous system of this mutant allows for non-invasive visualization of elongating nerve axons.

Donating Investigator

Dr. Kerry L. Tucker, Ruprecht Karls University of Heidelberg

Genetic overview

Genetic Background	Generation
	?+N2F11 (2019-06-12 00:00:00)

Mapt^tm1(EGFP)Klt

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Mapt	microtubule-associated protein tau

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Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$333.00 Domestic price for female

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Details

Detailed Description

Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A knock-in of the EGFP coding sequence into the first exon disrupts expression of the Mapt gene and produces a cytoplasmic EGFP fused to the first 31 amino acids. No gene product (isoform proteins) is detected in whole brain lysates by Western blot analysis. EGFP signal is detected beginning at 9.0 days post coitum in the trigeminal ganglion and by 10.75 days post coitum fluorescent signal is detected throughout the developing central nervous system. EGFP expression persists to adult and closely patterns the expression of neuron specific beta-tubulin III, as detected by the TuJ1 antibody. The expression of cytoplasmic EGFP in the central nervous system of this mutant allows for non-invasive visualization of elongating nerve axons.

Development

A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter and a EGFP (Clonetech) cDNA was inserted into exon 1. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were crossed to C57BL/6 mice. Heterozygotes were intercrossed to generate homozygotes. The mice were then crossed to a transgenic strain that was generated in embryos derived from a cross between Swiss Webster females and B6D2F1 males. This transgenic allele was subsequently removed from the strain during the development of our production stocks through selective breeding. These mice carry only the Mapt^tm1(EGFP)Klt allele.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP signal is detected beginning a embryonic day 9 in the trigeminal ganglion and throughout the developing central nervous system by embryonic day 10.75. GFP expression persists in adults and closely resembles the expression of neuron specific beta-tubulin III.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Tucker KL; Meyer M; Barde YA. 2001. Neurotrophins are required for nerve growth during development. Nat Neurosci 4(1):29-37PubMed: 11135642 MGI: J:66561

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Genetics

Mapt^tm1(EGFP)Klt

Allele Symbol: Mapt^tm1(EGFP)Klt

Allele Name	targeted mutation 1, Kerry Lee Tucker
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Kerry Lee Tucker; Mapt^tm1(EGFP)Klt
Gene Symbol and Name	Mapt, microtubule-associated protein tau
Gene Synonym(s)	RNPTAU; MSTD; FTDP-17; Mtapt; pTau; Mtapt; MTBT1; AI413597; PPND; PPP1R103; DDPAC; Tau; expressed sequence AI413597; MTBT2; expressed sequence AW045860; MAPTL; TAU; AW045860; Tau
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP signal is detected beginning a embryonic day 9 in the trigeminal ganglion and throughout the developing central nervous system by embryonic day 10.75. GFP expression persists in adults and closely resembles the expression of neuron specific beta-tubulin III.
Strain of Origin	129S4/SvJae
Chromosome	11
Molecular Note	Exon 1 was disrupted by the insertion of a cassette encoding the EGFP fluorescent marker gene and a neomycin resistance gene. Fusion protein including EGFP was detected by Western blot analysis of homozygous mutant brain lysates. Fluorescent illumination indicated that expression of the reporter gene was similar to that of the endogenous gene.
Mutations Made By	Dr. Kerry Tucker, Ruprecht Karls University of Heidelberg

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Fluorescent Proteins
- Genetics Research
  - Tissue/Cell Markers
  - Tissue/Cell Markers: neurons
- Neurobiology Research
  - cell marker
Neurobiology Research
- Fluorescent protein expression in neural tissue
- Alzheimer's Disease

Genotype: Mapt^tm1(EGFP)Klt related

Neurobiology Research
- Alzheimer's Disease
  - Tau (Mapt) mutants

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt
involves: 129S4/SvJae * C57BL/6

mammalian phenotype

behavior/neurological phenotype
- Normal - mice show no significant motor deficits up to 12 months of age
- Normal - spatial working memory is not impaired

normal phenotype

no abnormal phenotype detected
- Normal - homozygotes are viable and fertile with no detectable abnormalities in morphology of the central or peripheral nervous systems
- viable and fertile with no central or peripheral nervous system defects

Genotype: Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt
B6.129S4-Mapt

behavior/neurological phenotype

abnormal motor learning
- however, latency to fall is similar to wild-type
- no improvement in motor skills or learning is observed in 6 month old male mice on accelerating rotarod as compared to wild-type

enhanced spatial learning
- improved performance in spatial navigation (escape latency and path length)is observed in 6 month old male mice, but only in the second week (days 6-10) of Morris water maze testing

impaired contextual conditioning behavior
- 6 month old male mice freeze less during contextual fear conditioning as compared to wild-type

impaired cued conditioning behavior
- 6 month old male mice freeze less during cued fear conditioning as compared to wild-type

nervous system phenotype

reduced long term potentiation
- mice display a brief episode of potentiation but fail to develop long term potentiation following 3 repetitive theta bursts

References

Tucker KL; Meyer M; Barde YA. 2001. Neurotrophins are required for nerve growth during development. Nat Neurosci 4(1):29-37PubMed: 11135642 MGI: J:66561

Additional - Mapt^tm1(EGFP)Klt related

Technical Support

Contact Technical Support

Genotyping Protocols
- MELT: Mapt^tm1(EGFP)Klt Alternate 1
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, these mice may be bred as homozygotes. Expected coat color is Agouti and Black.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the Tau KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004779 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX11 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Mapt<tm1(EGFP)Klt>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Also Known As: Tau KO

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Mapttm1(EGFP)Klt

Allele Symbol: Mapttm1(EGFP)Klt

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Mapttm1(EGFP)Klt related

Mammalian Phenotype Terms by Genotype

Genotype: Mapttm1(EGFP)Klt/Mapttm1(EGFP)Kltinvolves: 129S4/SvJae * C57BL/6

mammalian phenotype

normal phenotype

Genotype: Mapttm1(EGFP)Klt/Mapttm1(EGFP)KltB6.129S4-Mapt

behavior/neurological phenotype

nervous system phenotype

References

Additional - Mapttm1(EGFP)Klt related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Mapt^tm1(EGFP)Klt

Allele Symbol: Mapt^tm1(EGFP)Klt

Genotype: Mapt^tm1(EGFP)Klt related

Genotype: Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt
involves: 129S4/SvJae * C57BL/6

Genotype: Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt
B6.129S4-Mapt

Additional - Mapt^tm1(EGFP)Klt related