These mice carry an ENU-induced mutation characterized by an unsteady, 'seal-like' gait.
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The mutants are small and show an intense body tremor with unsteady, 'seal-like' gait; their hind limbs appear weak, i.e. appear to carry the body weight with difficulty only, and sometimes one or the other hind limb lags behind. The phenotype can be observed at 3.5 weeks of age (+/-0.63; n=118). Mice of either gender are affected.
Standard pathology work-up on two mutants (27 or 28 days of age) revealed a hypoplastic spleen with atrophy of both lymphoid and hemaopoietic areas, and atrophy of the thymus in both mice. Hypoplastic bone marrow was noted in one, and fatty changes in the liver were noted in the other animal.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||neuroscience mutagenesis facility, 148|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||nmf148, neuroscience mutagenesis facility, 148|
|Strain of Origin||C57BL/6J|
|Molecular Note||This phenotypic mutant was identified in an ENU mutagenesis screen.|
A colony needs to be maintained through ovarian transplants and heterozygote matings.
When using the C57BL/6J-nmf148/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004765 in your Materials and Methods section.