B6.129S2-Myb^tm1Ssp/J

Stock number: 004757
Product name: c-myb-
Research areas: Developmental Biology Research, Hematological Research

Description

These Myb knock-out mice have an embryonic lethal phenotype with embryos exhibiting defective hematopoiesis.

Detailed description

Homozygous null mice have an embryonic lethal phenotype, failing to develop past embryonic days 15.5. At embryonic day 13, homozygotes are normal in size and morphology, but have 10 fold lower hematocrit levels, due to anemia caused by diminished fetal hepatic erthropoiesis. In vitro examination of cells from the para-aortic, splanchnopleural (P-Sp) and aorta-gonad-mesonephros regions reveals defective hematopoiesis. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of fetal definitive hematopoiesis.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 6. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for at least 6 generations.

Allele

Symbol: Myb^tm1Ssp
Name: targeted mutation 1, S Steven Potter
MGI accession ID: MGI:2662859
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Myb
Marker name: myeloblastosis oncogene
Chromosome: 10
Strain of origin: 129S2/SvPas
Molecular note: The endogenous locus was disrupted by the insertion of a neomycin selection cassette into exon 6.