These IL-Ira-Overexpressing mice express a transgene containing the sequence encoding the secreted mouse Il1rn protein with a point mutation (G to A) in exon 3, resulting in overexpression after lipopolysaccharide (LPS) endotoxin challenge.
Dr. Emmet Hirsch, Northwestern University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These transgenic mice overexpress the secreted mouse Il1rn under the control of endogenous regulatory elements. The transgene contains the sequence encoding the secreted protein with a point mutation (G to A) in exon 3. These mice have an estimated 8 copies of the transgene in tandem array. Northern blot analysis of liver tissue following lipopolysaccharide (LPS) endotoxin challenge detects overexpression. RT-PCR analysis of corneal tissue detects higher levels of gene product (mRNA) than wildtype controls. The Donating Investigator reports that hemizygous mice are viable and fertile, whereas homozygous mice are sickly and die prematurely. Transgenic mice are less susceptible to LPS challenge than wildtype controls, but are more susceptible to experimental Listeria monocytogenes infection. Following endotoxin (LPS) challenge serum IL-1 levels are higher. Mice hemizygous for the transgene exhibit an intermediate susceptibility. Severity of stromal keratitis caused by herpes simplex virus (HSV) ocular infection is diminished. Mice with HSV ocular infection display reduced angiogenesis in the cornea, reduced polymorphonuclear leukocyte infiltration, decreased levels of macrophage-inflammatory protein 2 (MIP-2), IL-6, vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 2 (VEGFR-2)levels, and delayed viral clearance. This mutant mouse strain may be useful in studies of inflammation and cytokine regulation.
A transgenic construct containing the sequence encoding the mouse Il1rn gene (secreted protein) with a site directed mutation (G to A) in exon 3 was injected into fertilized B6CBAF2 eggs. The resulting transgenic mice, founder line 14, were backcrossed to the B6CBAF1/J background for an unspecified number of generations before being backcrossed for more than 20 generations onto the C57BL/6J background.
Expressed Gene | Il1rn, interleukin 1 receptor antagonist, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1, David Hirsh |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | IL1ra Tg; IL-1ratg |
Gene Symbol and Name | Tg(Il1rn)1Dih, transgene insertion 1, David Hirsh |
Gene Synonym(s) | |
Promoter | Il1rn, interleukin 1 receptor antagonist, mouse, laboratory |
Expressed Gene | Il1rn, interleukin 1 receptor antagonist, mouse, laboratory |
Strain of Origin | (C57BL/6J x CBA)F2 |
Chromosome | UN |
Molecular Note | This transgene contains the sequence encoding the secreted gene protein and approximately 2.0kb of upstream and 2.5kb of downstream untranslated flanking regions, and a site directed mutation (G to A) in exon 3. Northern blot analysis of liver tissue following LPS challenge detects overexpression. Two lines were created and called T14 and T16. |
Mutations Made By | Dr. Emmet Hirsch, Northwestern University |
When maintaining a live colony, hemizygous mice are bred to wildtype siblings.
When using the IL-Ira-Overexpressing mouse strain in a publication, please cite the originating article(s) and include JAX stock #004753 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Il1rn)1Dih |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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