Overview

Peritoneal neutrophils and macrophages, bone marrow cells and neutrophils isolated from bone marrow of mice homozygous for Ncf1^m1J spontaneous mutation fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA).

Genetic overview

Genetic Background	Generation
	N3+N3F3 (2019-05-03 00:00:00)

Ncf1^m1J

Allele Type	Gene Symbol	Gene Name
Spontaneous	Ncf1	neutrophil cytosolic factor 1

View Genetics

Research Applications

Mouse/Human Gene Homologs
Research Tools
Hematological Research
Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$236.78 Domestic price for female 4-week

View Price List

Details

Detailed Description

Peritoneal neutrophils and macrophages, bone marrow cells and neutrophils isolated from bone marrow of mice homozygous for Ncf1^m1J fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA), as determined by kinetic spectrophotometric analysis of cytochrome c reduction. Western blot analysis detected no full-length NCF1/p47^phox protein in cells from these mice; a faint band of slightly smaller molecular size than the wild type NCF1 protein was observed on probing with antibodies to NCF1. To exclude the possibility that the NCF1 protein is produced in cells of mutant mice but is degraded rapidly by endogenous proteases, bone marrow cells were isolated and samples prepared for western blot analysis in the presence of diisopropyl fluorophosphate (DFP); no difference was observed upon analysis of freshly prepared cell lysates made with and without DFP, indicating that NCF1 protein is not expressed in mutant cells (Huang et al. 2000).

Analysis for other NADPH oxidases involved in neutrophil superoxide production revealed that NCF2/p67^phox was present at wild type levels and CYBB/gp91^phox and CYBA/p22^phox were expressed at higher than wild type levels. Other neutrophil products were assayed and found not to differ in bone marrow cells of C57BL/6J vs. mutant mice: GR-1, a granulocyte marker present on approximately 40% of marrow cells by fluorescence activated cell sorting (FACS) analysis; NCF4/p40^phox and RAC1/RAC2 proteins, analyzed by western blot analysis; and expression of mRNAs encoding the granule proteins myeloperoxidase, elastase, cathepsin, lysozyme, lactoferrin and gelatinase as detected by reverse transcriptase - polymerase chain reaction (RT-PCR) (Huang et al. 2000).

The response of Ncf1^m1J/Ncf1^m1J mice to infectious or irritant (inflammatory stimulus) challenge has not been investigated (Huang et al. 2000). However, mice homozygous for the targeted mutation Ncf1^tm1Shl (Stock No. 027331) - which disrupts the gene near the point of the Ncf1^m1J mutation and, like the latter, results in failure of phagocytes to generate a superoxide burst following PMA stimulation in vitro - developed spontaneous infections with a variety of microorganisms including Staphylococcus xylosus, Lactobacillus, a hyaline septate mold and the fungus Paecilomyces, despite being maintained under specific pathogen free conditions. Ten-fold more Staphyloccoccus aureus bacteria survived intracellularly following in vitro uptake by phagocytes inblood from these mice than from control littermates, demonstrating a defect in the intracellular bactericidal activity of the mutant mice. Following intraperitoneal injection of the sterile irritant thioglycolate, twice as many leukocytes were recruited to the peritoneal cavities of Ncf1^tm1Shl homozygous mice than of littermate controls; proportions of neutrophils in mutant and control exudates were similar (Jackson et al., J Exp Med 182:751-758 1995).

Development

This spontaneous mutation arose in the B6.BKS(D)-Lepr^db/J (Stock No. 000697) production colony at The Jackson Laboratory. The mutation was identified in 1999. (Huang CK. et al. 2000) The mutations Lepr^db and m have been selectively bred out of this strain by crossing to C57BL/6J at least three times.

Control Suggestions

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Huang CK; Zhan L; Hannigan MO; Ai Y; Leto TL. 2000. P47(phox)-deficient NADPH oxidase defect in neutrophils of diabetic mouse strains, C57BL/6J-m db/db and db/+. J Leukoc Biol 67(2):210-5PubMed: 10670582 MGI: J:83428

View All References

Genetics

Ncf1^m1J

Allele Symbol: Ncf1^m1J

Allele Name	mutation 1, Jackson
Allele Type	Spontaneous
Allele Synonym(s)	Ncf1^m1J; mutation 1, Jackson
Gene Symbol and Name	Ncf1, neutrophil cytosolic factor 1
Gene Synonym(s)	Ncf-1; NCF1A; NOXO2; NADPH oxidase subunit (47kDa); p47^phox; p47phox; meutrophil cytosolic factor 1; SH3PXD1A; Ncf-1
Strain of Origin	B6.Cg-Dock7^m +/+ Lepr^db/J
Chromosome	5
Molecular Note	The mutation is an A to C transversion at -2 position at the 5' end of exon 8 of the Ncf1 gene, resulting in aberrant splicing of the Ncf1 transcript. Immunoblotting detected no intact NCF1 protein in cells from these mice.

Disease/Phenotype

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

chronic granulomatous disease

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Ncf1^m1J related

Mouse/Human Gene Homologs
- granulomatous disease, chronic, autosomal cytochrome-b-positive form 1 (CGD)
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - neutrophil NADPH oxydase deficiency
Hematological Research
- Immunological Defects
- Neutrophil Defects
  - NADPH oxydase deficiency
Immunology, Inflammation and Autoimmunity Research
- Inflammation
  - Neutrophil defects
- Immunodeficiency
  - Neutrophil Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Ncf1^m1J/Ncf1^m1J
B6.Cg-Dock7 +/+ Lepr/J

hematopoietic system phenotype

abnormal macrophage physiology
- peritoneal macrophages fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA)

impaired granulocyte bactericidal activity
- peritoneal neutrophils, bone marrow cells and neutrophils isolated from bone marrow fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA)

immune system phenotype

abnormal macrophage physiology
- peritoneal macrophages fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA)

decreased susceptibility to viral infection
- following exposure to inactivated H5N1 virus, acute-lung injury is attenuated compared to in wild-type mice

impaired granulocyte bactericidal activity
- peritoneal neutrophils, bone marrow cells and neutrophils isolated from bone marrow fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA)

Genotype: Ncf1^m1J/Ncf1^m1J
involves: C57BL/6

cellular phenotype

oxidative stress
- in the sinoatrial node of STZ-treated mice

References

Huang CK; Zhan L; Hannigan MO; Ai Y; Leto TL. 2000. P47(phox)-deficient NADPH oxidase defect in neutrophils of diabetic mouse strains, C57BL/6J-m db/db and db/+. J Leukoc Biol 67(2):210-5PubMed: 10670582 MGI: J:83428

Additional References

Hultqvist M; Olofsson P; Holmberg J; Backstrom BT; Tordsson J; Holmdahl R. 2004. Enhanced autoimmunity, arthritis, and encephalomyelitis in mice with a reduced oxidative burst due to a mutation in the Ncf1 gene. Proc Natl Acad Sci U S A 101(34):12646-51PubMed: 15310853 MGI: J:92437

Additional - Ncf1^m1J related

Technical Support

Contact Technical Support

Genotyping Protocols
- Restriction Enzyme Digest: Ncf1^m1J
- Pyrosequencing: Ncf1^m1J
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Mating System
- Homozygote x Homozygote
Citation
When using the B6(Cg)-Ncf1^m1J/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004742 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Ncf1<m1J>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Ncf1m1J

Allele Symbol: Ncf1m1J

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Ncf1m1J related

Mammalian Phenotype Terms by Genotype

Genotype: Ncf1m1J/Ncf1m1JB6.Cg-Dock7 +/+ Lepr/J

hematopoietic system phenotype

immune system phenotype

Genotype: Ncf1m1J/Ncf1m1Jinvolves: C57BL/6

cellular phenotype

References

Additional References

Additional - Ncf1m1J related

Genotyping Protocols

Dietary Information

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Ncf1^m1J

Allele Symbol: Ncf1^m1J

Genotype: Ncf1^m1J related

Genotype: Ncf1^m1J/Ncf1^m1J
B6.Cg-Dock7 +/+ Lepr/J

Genotype: Ncf1^m1J/Ncf1^m1J
involves: C57BL/6

Additional - Ncf1^m1J related