Mice carry a transgenic TCR allowing the examination of T-cell tolerance to two independent antigens with the same transgenic mouse line.
Dr. David Serreze, The Jackson Laboratory
Genetic Background | Generation |
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N2F8p+F13
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These mice carry a T-cell receptor (Tcra-V2, Tcra-J TA31 / Tcrb-V8.1, Tcrb-D, Tcrb-J 2.4) specific for LCMV (lymphocytic choriomeningitis virus), H2-Db . They offer the unique possibility to examine T-cell tolerance to two independent antigens with the same transgenic mouse line. First, T-cell tolerance to LCMV has been studied in transgenic mice carrying the non-cytopathic LCM virus after neonatal infection. Second, the transgenic TCR use the beta-chain variable gene segment Tcrb-V8.1 that reacts preferentially with Mlsa (mixed lymphocyte stimulatory) antigen. Ten to twenty copies of the Tcr transgene are reportedly integrated on the same chromosome. The animals are on a mixed C57BL/6 and DBA/2 background but the MHC haplotype has been checked and found to be H2b.
Zinkernagel et al, established a set of LCMV-specific cytotoxic T-cell clones from lethally irradiated B10.BR/SgSn(H-2K reconstituted with T-cell depleted C57BL/10Sn (H-2bbone marrow cells. The rearranged TCR alpha (Tcra-V2 and Tcra-J TA31) and beta (Tcrb-V8.1, TcrB-D, TcrB-J2.4) chain gene sequences from clone P14 were incorporated into transgene contructs regulated by the H-2Kbpromoter and co-injected into B6D2F2 fertilized embryos. 10-20 copies of both the alpha and beta transgenes co-integrated on the same chromosome in male founder number 327. Founder 327 was backcrossed to C57BL/6J for one generation prior to being intercrossed to make this a homozygous strain. This strain is maintained by homozygous sibling matings.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion 327, Birgit Ledermann |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | P14; P14 TCR; P14 TCR-alphabeta (Valpha2Vbeta8.1); P14 TCRValpha2Vbeta8; P14-TCR; TCR LCMV; TCR P14; TCR-Tg; TgTcr |
Gene Symbol and Name | Tg(TcrLCMV)327Sdz, transgene insertion 327, Birgit Ledermann |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
General Note | Transgenic mice carry a T-cell receptor (Tcra-V2, Tcra-J TA31 / Tcrb-V8.1, Tcrb-D, Tcrb-J 2.4) specific for the gp33 protein of LCMV (lymphocytic choriomeningitis virus), H2-Db. |
Molecular Note | The transgene carries both Tcra and Tcrb. Ten to twenty copies of the Tcr transgene were reported to have integrated on the same chromosome. At least two transgenic lines are known to exist. Line 327 is the representative line. The second line is 318. |
Mutations Made By | Dr. Rolf Zinkernagel, University of Zurich |
While maintaining a live colony, homozygous mice may be mated together.
When using the B6;D2-TCR LCMV mouse strain in a publication, please cite the originating article(s) and include JAX stock #004694 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Homozygous for Tg(TcrLCMV)327Sdz, 1 pair minimum |
Frozen Mouse Embryo | B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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