cellular phenotype

• decreased T cell proliferation
  • response of uninfected transgenic Mls^a spleen cells to LCMV is reduced ~3-fold
  (MGI Ref ID J:77696)

endocrine/exocrine gland phenotype

• thymus hypoplasia
  • numbers of double-positive thymocytes and CD8+ thymocytes are drastically reduced in transgenic mice
  (MGI Ref ID J:77696)

hematopoietic system phenotype

• decreased CD8-positive, alpha-beta T cell number
  • number of CD8+ T cells in spleens of transgenic Mls^a mice is reduced 4-fold compared to uninfected transgenic Mls^b but ~4-fold higher than LCMV-infected Mls^b mice
  (MGI Ref ID J:77696)

• decreased cytotoxic T cell cytolysis
  • LCMV-infected Mls^b show decreased significantly reduced proliferation and specific lysis compared to uninfected transgenic mice
  (MGI Ref ID J:77696)
  • lysis of LCMV-infected target cells is decreased ~3-fold in uninfected transgenic mice positive for mixed-lymphocyte stimulatory (Mls^a) antigen compared to transgenic mice positive for Mls^b antigen
  (MGI Ref ID J:77696)

• thymus hypoplasia
  • numbers of double-positive thymocytes and CD8+ thymocytes are drastically reduced in transgenic mice
  (MGI Ref ID J:77696)

• decreased T cell proliferation
  • response of uninfected transgenic Mls^a spleen cells to LCMV is reduced ~3-fold
  (MGI Ref ID J:77696)

• decreased double-positive T cell number
  • numbers of CD4+CD8+ thymocytes are drastically reduced in LCMV-tolerant (carrier) transgenic mice
  (MGI Ref ID J:77696)

immune system phenotype

• decreased CD8-positive, alpha-beta T cell number
  • number of CD8+ T cells in spleens of transgenic Mls^a mice is reduced 4-fold compared to uninfected transgenic Mls^b but ~4-fold higher than LCMV-infected Mls^b mice
  (MGI Ref ID J:77696)

• decreased cytotoxic T cell cytolysis
  • lysis of LCMV-infected target cells is decreased ~3-fold in uninfected transgenic mice positive for mixed-lymphocyte stimulatory (Mls^a) antigen compared to transgenic mice positive for Mls^b antigen
  (MGI Ref ID J:77696)
  • LCMV-infected Mls^b show decreased significantly reduced proliferation and specific lysis compared to uninfected transgenic mice
  (MGI Ref ID J:77696)

• thymus hypoplasia
  • numbers of double-positive thymocytes and CD8+ thymocytes are drastically reduced in transgenic mice
  (MGI Ref ID J:77696)

• decreased T cell proliferation
  • response of uninfected transgenic Mls^a spleen cells to LCMV is reduced ~3-fold
  (MGI Ref ID J:77696)

• decreased double-positive T cell number
  • numbers of CD4+CD8+ thymocytes are drastically reduced in LCMV-tolerant (carrier) transgenic mice
  (MGI Ref ID J:77696)

hematopoietic system phenotype

• abnormal T cell differentiation
  • there is only minimal rearrangement of endogenous TCRalpha chains needed in double-positive thymocytes to express a transgenic TCR, so that mice have very few CD4 single-positive thymocytes or CD4⁺ peripheral T cells
  (MGI Ref ID J:97610)
  • T cells expressing the transgene preferentially develop into CD8⁺ peripheral T cells
  (MGI Ref ID J:97610)

• decreased CD4-positive, alpha beta T cell number
  • very few CD4⁺T cells are found in the thymus or periphery
  (MGI Ref ID J:97610)

immune system phenotype

• decreased CD4-positive, alpha beta T cell number
  • very few CD4⁺T cells are found in the thymus or periphery
  (MGI Ref ID J:97610)

• abnormal T cell differentiation
  • T cells expressing the transgene preferentially develop into CD8⁺ peripheral T cells
  (MGI Ref ID J:97610)
  • there is only minimal rearrangement of endogenous TCRalpha chains needed in double-positive thymocytes to express a transgenic TCR, so that mice have very few CD4 single-positive thymocytes or CD4⁺ peripheral T cells
  (MGI Ref ID J:97610)