B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ

Stock number: 004694
Product name: B6;D2-TCR LCMV
Research areas: Research Tools

Description

Mice carry a transgenic TCR allowing the examination of T-cell tolerance to two independent antigens with the same transgenic mouse line.

Detailed description

These mice carry a T-cell receptor (Tcra-V2, Tcra-J TA31 / Tcrb-V8.1, Tcrb-D, Tcrb-J 2.4) specific for LCMV (lymphocytic choriomeningitis virus), H2-Db . They offer the unique possibility to examine T-cell tolerance to two independent antigens with the same transgenic mouse line. First, T-cell tolerance to LCMV has been studied in transgenic mice carrying the non-cytopathic LCM virus after neonatal infection. Second, the transgenic TCR use the beta-chain variable gene segment Tcrb-V8.1 that reacts preferentially with Mls^a (mixed lymphocyte stimulatory) antigen. Ten to twenty copies of the Tcr transgene are reportedly integrated on the same chromosome. The animals are on a mixed C57BL/6 and DBA/2 background but the MHC haplotype has been checked and found to be H2b.

Development

Zinkernagel et al, established a set of LCMV-specific cytotoxic T-cell clones from lethally irradiated B10.BR/SgSn(H-2^K reconstituted with T-cell depleted C57BL/10Sn (H-2^bbone marrow cells. The rearranged TCR alpha (Tcra-V2 and Tcra-J TA31) and beta (Tcrb-V8.1, TcrB-D, TcrB-J2.4) chain gene sequences from clone P14 were incorporated into transgene contructs regulated by the H-2K^bpromoter and co-injected into B6D2F2 fertilized embryos. 10-20 copies of both the alpha and beta transgenes co-integrated on the same chromosome in male founder number 327. Founder 327 was backcrossed to C57BL/6J for one generation prior to being intercrossed to make this a homozygous strain. This strain is maintained by homozygous sibling matings.

Allele

Symbol: Tg(TcrLCMV)327Sdz
Name: transgene insertion 327, Birgit Ledermann
MGI accession ID: MGI:2665105
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(TcrLCMV)327Sdz
Marker name: transgene insertion 327, Birgit Ledermann
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F2
Expressed genes: Tcrb,T cell receptor beta chain,mouse, laboratory; Tcra,T cell receptor alpha chain,mouse, laboratory
Molecular note: The transgene carries both Tcra and Tcrb. Ten to twenty copies of the Tcr transgene were reported to have integrated on the same chromosome. At least two transgenic lines are known to exist. Line 327 is the representative line. The second line is 318.
General note: Transgenic mice carry a T-cell receptor (Tcra-V2, Tcra-J TA31 / Tcrb-V8.1, Tcrb-D, Tcrb-J 2.4) specific for the gp33 protein of LCMV (lymphocytic choriomeningitis virus), H2-D^b.