JAX Mouse Strain Datasheet - 004686

B6;129S4-Tsc2^tm1Djk/J

Stock No:: 004686

Targeted Mutation

Cryo Recovery

Overview

Tsc2+/- mice are useful in studying Tuberous sclerosis. In addition, the mTOR hyperactivation exhibited by Tsc2+/- mice causes impaired basal neuronal autophagy, resulting in autism spectrum disorder (ASD)-like basal dendritic spine pathology and social recognition/interaction deficits.

Donating Investigator

David J. Kwiatkowski, Brigham and Women's Hospital

Genetic overview

Genetic Background	Generation

Tsc2^tm1Djk

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Tsc2	tuberous sclerosis 2

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Research Applications

Cancer Research
Developmental Biology Research

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Exon 2 of the tuberous sclerosis 2 gene (Tsc2) has been replaced by a neo cassette, abolishing gene expression. TSC2 is a putative tumor supressor. Mutation of TSC2 have been associated with the onset of tuberous sclerosis complex (TSC) which is characterized by the formation of non-malignant tumors in many different organs. Heterozygous (Tsc2+/-) mice are viable and fertile. Homozygous (Tsc2-/-) mice have an embryonic lethal phenotype, failing to develop past embryonic days 9.5 to 12.5 due to hepatic hypoplasia. Cultured neuroepithelial progenitor cells isolated from embryonic day 10.5 embryos display abnormal growth and differentiation. All heterozygotes develop multiple bilateral renal cystadenomas by 12-15 months of age. By 15 months, about half develop liver hemangiomas (more common in females than in males). Less than 10% develop extremity angiosarcomas or renal carcinoma. Little or no gene product (protein) is detected by Western blot in renal cystadenomas. PCR analysis reveals loss of the wildtype allele in about 30% of lesions. Phenotype variability is dependent on genetic background.
Tsc2 haploinsufficiency causes mTOR hyperactivation: the subsequent autophagy inhibition results in autism spectrum disorder (ASD)-like basal dendritic spine pathology and social recognition/interaction deficits. Rapamycin treatment rescues the dendritic spine pruning defect and social abnormalities. Tsc2+/- mice do not exhibit stereotyped/repetitive behavior, motor defects or anxiety-like behavior.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 2 of the tuberous sclerosis 2 locus on chromosome 17. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygotes were then intercrossed.

Control Suggestions

Wild-type from the colony

Approximate Controls

101045 B6129SF2/J

Additional Information

Considerations for Choosing Controls

Selected References

Onda H; Lueck A; Marks PW; Warren HB; Kwiatkowski DJ. 1999. Tsc2(+/-) mice develop tumors in multiple sites that express gelsolin and are influenced by genetic background. J Clin Invest 104(6):687-95. PubMed: 10491404 MGI: J:57631

View All References

Genetics

Tsc2^tm1Djk

Allele Symbol: Tsc2^tm1Djk

Allele Name	targeted mutation 1, David J Kwiatkowski
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Tsc2^-; Tsc^-
Gene Symbol and Name	Tsc2, tuberous sclerosis 2
Gene Synonym(s)	LAM; Nafld; PPP1R160; Rc; TSC4; tuberin
Strain of Origin	129S4/SvJae
Chromosome	17
Molecular Note	A neomycin cassette was inserted into the second coding exon of the gene.
Mutations Made By	David Kwiatkowski, Brigham and Women's Hospital

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cancer Research
- Increased Tumor Incidence
  - Other Tissues/Organs: multiple
Developmental Biology Research
- Embryonic Lethality (Homozygous)

Genotype: Tsc2^tm1Djk related

Cancer Research
- Increased Tumor Incidence
  - Adenomas
  - Other Tissues/Organs
  - Other Tissues/Organs: lung

Mammalian Phenotype Terms by Genotype

Genotype: Tsc2^tm1Djk/Tsc2⁺
involves: 129S4/SvJae * C57BL/6NCrl

behavior/neurological phenotype

abnormal contextual conditioning behavior
- following contextual conditioning, mice exhibit reduced freezing compared to wild-type mice
- however, treatment with rapamycin re-establishes normal contextual conditioning

abnormal spatial learning
- however, spatial learning in a hippocampus-independent water maze is normal and treatment with rapamycin re-establishes normal spatial learning
- in a hippocampus-dependent version of the Morris water maze test, spatial learning is impaired compared to in wild-type mice
- mice exhibit more across-phase errors compared to in wild-type mice in a hippocampus-dependent win-shift version of the eight-arm radial maze

behavior/neurological phenotype
- mice exhibit normal motor skills, exploratory behavior, anxiety and social approach behavior

nervous system phenotype

enhanced long term potentiation
- however, treatment with rapamycin re-establishes normal late-phase long term potentiation
- mice exhibit lowered late-phase threshold for long term potentiation compared to in wild-type mice

nervous system phenotype
- mice exhibit normal basal synaptic transmission, paired-pulse facilitation and early-phase long term potentiation

Genotype: Tsc2^tm1Djk/Tsc2⁺
involves: 129S4/SvJae * C57BL/6J

behavior/neurological phenotype

abnormal response to novel object
- however, male adolescent mice do not exhibit repetitive behaviors, motor defects or anxiety-like behaviors in the open field
- male adolescent mice spend less time exploring the novel object than wild-type mice in the novel object recognition test

abnormal response to social novelty
- in the social novelty test, adolescent males spend a similar amount of time sniffing both novel and familiar targets, with decreased preference index (the ratio of time sniffing a stranger mouse versus a familiar mouse), indicating a reduced preference for social novelty
- male adolescent (P30-P35) mice spend less time sniffing the stimulus mouse during a dyadic social interaction with a novel mouse, indicating impaired social interactions

abnormal social investigation
- in the three-chamber social test, male adolescent mice show a preference for interacting with a social target compared with nonsocial target, and the preference index (the ratio of time sniffing mouse versus nonsocial target) is decreased
- treatment with rapamycin rescues social deficits (normalizes sociability and social novelty preferences

cellular phenotype

abnormal autophagy
- accumulation of lipid droplets and damaged mitochondria in primary neuronal cultures, indicating impaired autophagy
- basal autophagy is suppressed in the brain
- rapamycin treatment normalizes autophagy

nervous system phenotype

abnormal dendritic spine morphology
- density of dendritic spines in pyramidal neuron basal dendrites of layer V A1/S2 in temporal cortex is increased in adolescent males
- mice treated with an intraperitoneal injection of rapamycin show a correction of the pruning defect
- similar numbers of spines are seen at P19-P20 as in wild-type mice but far more spines in P29-P30 mutants, indicating a lack of normal spine pruning, with only 26% of spines being pruned

Genotype: Tsc2^tm1Djk/Tsc2⁺
either: (involves: 129S4/SvJae * BALB/cJ) or (involves: 129S4/SvJae * Black Swiss) or (involves: 129S4/SvJae * C57BL/6J)

liver/biliary system phenotype

increased hepatic hemangioma incidence
- 50% incidence; causes fatal bleeding in 10% of these cases

respiratory system phenotype

increased lung adenoma incidence
- 32% incidence

renal/urinary system phenotype

increased renal carcinoma incidence
- less than 10% incidence

increased renal cystadenoma incidence
- 100% incidence

neoplasm

increased extremity angiosarcoma incidence
- less than 10% incidence

increased hepatic hemangioma incidence
- 50% incidence; causes fatal bleeding in 10% of these cases

increased lung adenoma incidence
- 32% incidence

increased renal carcinoma incidence
- less than 10% incidence

increased renal cystadenoma incidence
- 100% incidence

increased tumor incidence
- Background Sensitivity - note that the tumor expression pattern is influenced by genetic background; authors note fewer large renal cystadenomas in outbred Black Swiss background and more angiosarcomas in 129S4/SvJae chimeric mice
- note that the tumor expression pattern is influenced by genetic background; authors note fewer large renal cystadenomas in outbred Black Swiss background and more angiosarcomas in 129S4/SvJae chimeric mice
- reported at 15 months of age

Genotype: Tsc2^tm1Djk/Tsc2^tm1Djk
either: (involves: 129S4/SvJae * BALB/cJ) or (involves: 129S4/SvJae * Black Swiss) or (involves: 129S4/SvJae * C57BL/6J)

mortality/aging

embryonic lethality during organogenesis, complete penetrance
- age of onset E9.5

liver/biliary system phenotype

liver hypoplasia

References

Onda H; Lueck A; Marks PW; Warren HB; Kwiatkowski DJ. 1999. Tsc2(+/-) mice develop tumors in multiple sites that express gelsolin and are influenced by genetic background. J Clin Invest 104(6):687-95. PubMed: 10491404 MGI: J:57631

Additional References

Uhlmann EJ; Apicelli AJ; Baldwin RL; Burke SP; Bajenaru ML; Onda H; Kwiatkowski D; Gutmann DH. 2002. Heterozygosity for the tuberous sclerosis complex (TSC) gene products results in increased astrocyte numbers and decreased p27-Kip1 expression in TSC2+/- cells. Oncogene 21(25):4050-9. PubMed: 12037687 MGI: J:77179

Additional - Tsc2^tm1Djk related

Auerbach BD; Osterweil EK; Bear MF. 2011. Mutations causing syndromic autism define an axis of synaptic pathophysiology. Nature 480(7375):63-8. PubMed: 22113615 MGI: J:178293
Bae SH; Sung SH; Oh SY; Lim JM; Lee SK; Park YN; Lee HE; Kang D; Rhee SG. 2013. Sestrins activate Nrf2 by promoting p62-dependent autophagic degradation of Keap1 and prevent oxidative liver damage. Cell Metab 17(1):73-84. PubMed: 23274085 MGI: J:195093
Blando J; Portis M; Benavides F; Alexander A; Mills G; Dave B; Conti CJ; Kim J; Walker CL. 2009. PTEN deficiency is fully penetrant for prostate adenocarcinoma in C57BL/6 mice via mTOR-dependent growth. Am J Pathol 174(5):1869-79. PubMed: 19395652 MGI: J:148013
Bonnet CS; Aldred M; von Ruhland C; Harris R; Sandford R; Cheadle JP. 2009. Defects in cell polarity underlie TSC and ADPKD-associated cystogenesis. Hum Mol Genet 18(12):2166-76. PubMed: 19321600 MGI: J:148543
Brown AB; Mahmood U; Cortes ML; Tang Y; Dai G; Stemmer-Rachamimov A; Prabhakar S; Leishear K; Onda H; Kwiatkowski D; Weissleder R; Breakefield X. 2005. Magnetic resonance imaging and characterization of spontaneous lesions in a transgenic mouse model of tuberous sclerosis as a model for endothelial cell-based transgene delivery. Hum Gene Ther 16(12):1367-76. PubMed: 16390268 MGI: J:107438
Caccamo A; Magri A; Medina DX; Wisely EV; Lopez-Aranda MF; Silva AJ; Oddo S. 2013. mTOR regulates tau phosphorylation and degradation: implications for Alzheimer's disease and other tauopathies. Aging Cell 12(3):370-80. PubMed: 23425014 MGI: J:218147
Cao J; Gong L; Guo DC; Mietzsch U; Kuang SQ; Kwartler CS; Safi H; Estrera A; Gambello MJ; Milewicz DM. 2010. Thoracic aortic disease in tuberous sclerosis complex: molecular pathogenesis and potential therapies in Tsc2+/- mice. Hum Mol Genet 19(10):1908-20. PubMed: 20159776 MGI: J:159339
Chen N; Debnath J. 2013. IkappaB kinase complex (IKK) triggers detachment-induced autophagy in mammary epithelial cells independently of the PI3K-AKT-MTORC1 pathway. Autophagy 9(8):1214-27. PubMed: 23778976 MGI: J:238448
D'Armiento J; Shiomi T; Marks S; Geraghty P; Sankarasharma D; Chada K. 2016. Mesenchymal Tumorigenesis Driven by TSC2 Haploinsufficiency Requires HMGA2 and Is Independent of mTOR Pathway Activation. Cancer Res 76(4):844-54. PubMed: 26837766 MGI: J:235545
Dibble CC; Elis W; Menon S; Qin W; Klekota J; Asara JM; Finan PM; Kwiatkowski DJ; Murphy LO; Manning BD. 2012. TBC1D7 Is a Third Subunit of the TSC1-TSC2 Complex Upstream of mTORC1. Mol Cell 47(4):535-46. PubMed: 22795129 MGI: J:187614
Dodd KM; Yang J; Shen MH; Sampson JR; Tee AR. 2015. mTORC1 drives HIF-1alpha and VEGF-A signalling via multiple mechanisms involving 4E-BP1, S6K1 and STAT3. Oncogene 34(17):2239-50. PubMed: 24931163 MGI: J:221262
Ehninger D; Han S; Shilyansky C; Zhou Y; Li W; Kwiatkowski DJ; Ramesh V; Silva AJ. 2008. Reversal of learning deficits in a Tsc2+/- mouse model of tuberous sclerosis. Nat Med 14(8):843-8. PubMed: 18568033 MGI: J:138621
Gilbert ER; Eby JM; Hammer AM; Klarquist J; Christensen DG; Barfuss AJ; Boissy RE; Picken MM; Love RB; Dilling DF; Le Poole IC. 2013. Positioning ganglioside d3 as an immunotherapeutic target in lymphangioleiomyomatosis. Am J Pathol 183(1):226-34. PubMed: 23665200 MGI: J:197440
Guo Y; Kwiatkowski DJ. 2013. Equivalent benefit of rapamycin and a potent mTOR ATP-competitive inhibitor, MLN0128 (INK128), in a mouse model of tuberous sclerosis. Mol Cancer Res 11(5):467-73. PubMed: 23386687 MGI: J:205463
Hartman TR; Liu D; Zilfou JT; Robb V; Morrison T; Watnick T; Henske EP. 2009. The tuberous sclerosis proteins regulate formation of the primary cilium via a rapamycin-insensitive and polycystin 1-independent pathway. Hum Mol Genet 18(1):151-63. PubMed: 18845692 MGI: J:143404
Huang J; Wu S; Wu CL; Manning BD. 2009. Signaling events downstream of mammalian target of rapamycin complex 2 are attenuated in cells and tumors deficient for the tuberous sclerosis complex tumor suppressors. Cancer Res 69(15):6107-14. PubMed: 19602587 MGI: J:150957
Kwiatkowski DJ; Zhang H; Bandura JL; Heiberger KM; Glogauer M; el-Hashemite N; Onda H. 2002. A mouse model of TSC1 reveals sex-dependent lethality from liver hemangiomas, and up-regulation of p70S6 kinase activity in Tsc1 null cells. Hum Mol Genet 11(5):525-34. PubMed: 11875047 MGI: J:75243
Lee L; Sudentas P; Donohue B; Asrican K; Worku A; Walker V; Sun Y; Schmidt K; Albert MS; El-Hashemite N; Lader AS; Onda H; Zhang H; Kwiatkowski DJ; Dabora SL. 2005. Efficacy of a rapamycin analog (CCI-779) and IFN-gamma in tuberous sclerosis mouse models. Genes Chromosomes Cancer 42(3):213-27. PubMed: 15578690 MGI: J:95229
Ma L; Teruya-Feldstein J; Behrendt N; Chen Z; Noda T; Hino O; Cordon-Cardo C; Pandolfi PP. 2005. Genetic analysis of Pten and Tsc2 functional interactions in the mouse reveals asymmetrical haploinsufficiency in tumor suppression. Genes Dev 19(15):1779-86. PubMed: 16027168 MGI: J:100094
Mak BC; Kenerson HL; Aicher LD; Barnes EA; Yeung RS. 2005. Aberrant beta-catenin signaling in tuberous sclerosis. Am J Pathol 167(1):107-16. PubMed: 15972957 MGI: J:99509
Manning BD; Logsdon MN; Lipovsky AI; Abbott D; Kwiatkowski DJ; Cantley LC. 2005. Feedback inhibition of Akt signaling limits the growth of tumors lacking Tsc2. Genes Dev 19(15):1773-8. PubMed: 16027169 MGI: J:100095
Nie D; Di Nardo A; Han JM; Baharanyi H; Kramvis I; Huynh T; Dabora S; Codeluppi S; Pandolfi PP; Pasquale EB; Sahin M. 2010. Tsc2-Rheb signaling regulates EphA-mediated axon guidance. Nat Neurosci 13(2):163-72. PubMed: 20062052 MGI: J:156683
Obayashi Y; Campbell JS; Fausto N; Yeung RS. 2013. Impaired lipid accumulation in the liver of Tsc2-heterozygous mice during liver regeneration. Biochem Biophys Res Commun 437(1):146-50. PubMed: 23810393 MGI: J:205324
Onda H; Crino PB; Zhang H; Murphey RD; Rastelli L; Gould Rothberg BE; Kwiatkowski DJ. 2002. Tsc2 Null Murine Neuroepithelial Cells Are a Model for Human Tuber Giant Cells, and Show Activation of an mTOR Pathway. Mol Cell Neurosci 21(4):561-74. PubMed: 12504590 MGI: J:81245
Parkhitko A; Myachina F; Morrison TA; Hindi KM; Auricchio N; Karbowniczek M; Wu JJ; Finkel T; Kwiatkowski DJ; Yu JJ; Henske EP. 2011. Tumorigenesis in tuberous sclerosis complex is autophagy and p62/sequestosome 1 (SQSTM1)-dependent. Proc Natl Acad Sci U S A 108(30):12455-60. PubMed: 21746920 MGI: J:174534
Pollizzi K; Malinowska-Kolodziej I; Doughty C; Betz C; Ma J; Goto J; Kwiatkowski DJ. 2009. A hypomorphic allele of Tsc2 highlights the role of TSC1/TSC2 in signaling to AKT and models mild human TSC2 alleles. Hum Mol Genet 18(13):2378-87. PubMed: 19357198 MGI: J:149326
Pollizzi K; Malinowska-Kolodziej I; Stumm M; Lane H; Kwiatkowski D. 2009. Equivalent benefit of mTORC1 blockade and combined PI3K-mTOR blockade in a mouse model of tuberous sclerosis. Mol Cancer 8:38. PubMed: 19527517 MGI: J:157342
Potter WB; Basu T; O'Riordan KJ; Kirchner A; Rutecki P; Burger C; Roopra A. 2013. Reduced juvenile long-term depression in tuberous sclerosis complex is mitigated in adults by compensatory recruitment of mGluR5 and Erk signaling. PLoS Biol 11(8):e1001627. PubMed: 23966835 MGI: J:201589
Settembre C; Zoncu R; Medina DL; Vetrini F; Erdin S; Erdin S; Huynh T; Ferron M; Karsenty G; Vellard MC; Facchinetti V; Sabatini DM; Ballabio A. 2012. A lysosome-to-nucleus signalling mechanism senses and regulates the lysosome via mTOR and TFEB. EMBO J 31(5):1095-108. PubMed: 22343943 MGI: J:182149
Short JD; Houston KD; Dere R; Cai SL; Kim J; Johnson CL; Broaddus RR; Shen J; Miyamoto S; Tamanoi F; Kwiatkowski D; Mills GB; Walker CL. 2008. AMP-activated protein kinase signaling results in cytoplasmic sequestration of p27. Cancer Res 68(16):6496-506. PubMed: 18701472 MGI: J:139145
Sun Y; Gallacchi D; Zhang EY; Reynolds SB; Robinson L; Malinowska IA; Chiou TT; Pereira AM; Li C; Kwiatkowski DJ; Lee PS; Yu JJ. 2014. Rapamycin-resistant poly (ADP-ribose) polymerase-1 overexpression is a potential therapeutic target in lymphangioleiomyomatosis. Am J Respir Cell Mol Biol 51(6):738-49. PubMed: 24874429 MGI: J:232230
Switon K; Kotulska K; Janusz-Kaminska A; Zmorzynska J; Jaworski J. 2017. Molecular neurobiology of mTOR. Neuroscience 341:112-153. PubMed: 27889578 MGI: J:238268
Tang G; Gudsnuk K; Kuo SH; Cotrina ML; Rosoklija G; Sosunov A; Sonders MS; Kanter E; Castagna C; Yamamoto A; Yue Z; Arancio O; Peterson BS; Champagne F; Dwork AJ; Goldman J; Sulzer D. 2014. Loss of mTOR-dependent macroautophagy causes autistic-like synaptic pruning deficits. Neuron 83(5):1131-43. PubMed: 25155956 MGI: J:217829
Uhlmann EJ; Apicelli AJ; Baldwin RL; Burke SP; Bajenaru ML; Onda H; Kwiatkowski D; Gutmann DH. 2002. Heterozygosity for the tuberous sclerosis complex (TSC) gene products results in increased astrocyte numbers and decreased p27-Kip1 expression in TSC2+/- cells. Oncogene 21(25):4050-9. PubMed: 12037687 MGI: J:77179
Wang CY; X738b X798e X9685; Stapleton DS; Schueler KL; Rabaglia ME; Oler AT; Keller MP; Kendziorski CM; Broman KW; Yandell BS; Schadt EE; Attie AD. 2012. Tsc2, a positional candidate gene underlying a quantitative trait locus for hepatic steatosis. J Lipid Res 53(8):1493-1501. PubMed: 22628617 MGI: J:186560
Wang F; Chen X; Li C; Sun Q; Chen Y; Wang Y; Peng H; Liu Z; Chen R; Liu K; Yan H; Ye BH; Kwiatkowski DJ; Zhang H. 2014. Pivotal role of augmented alphaB-crystallin in tumor development induced by deficient TSC1/2 complex. Oncogene 33(34):4352-8. PubMed: 24077282 MGI: J:214354
Yang J; Kalogerou M; Gallacher J; Sampson JR; Shen MH. 2013. Renal tumours in a Tsc1+/- mouse model show epigenetic suppression of organic cation transporters Slc22a1, Slc22a2 and Slc22a3, and do not respond to metformin. Eur J Cancer 49(6):1479-90. PubMed: 23228442 MGI: J:196914
Yang J; Kalogerou M; Samsel PA; Zhang Y; Griffiths DF; Gallacher J; Sampson JR; Shen MH. 2015. Renal tumours in a Tsc2(+/-) mouse model do not show feedback inhibition of Akt and are effectively prevented by rapamycin. Oncogene 34(7):922-31. PubMed: 24632604 MGI: J:218622
Young DM; Schenk AK; Yang SB; Jan YN; Jan LY. 2010. Altered ultrasonic vocalizations in a tuberous sclerosis mouse model of autism. Proc Natl Acad Sci U S A 107(24):11074-9. PubMed: 20534473 MGI: J:161396
Yuan E; Tsai PT; Greene-Colozzi E; Sahin M; Kwiatkowski DJ; Malinowska IA. 2012. Graded loss of tuberin in an allelic series of brain models of TSC correlates with survival, and biochemical, histological and behavioral features. Hum Mol Genet 21(19):4286-300. PubMed: 22752306 MGI: J:187405
Zhang H; Cicchetti G; Onda H; Koon HB; Asrican K; Bajraszewski N; Vazquez F; Carpenter CL; Kwiatkowski DJ. 2003. Loss of Tsc1/Tsc2 activates mTOR and disrupts PI3K-Akt signaling through downregulation of PDGFR. J Clin Invest 112(8):1223-33. PubMed: 14561707 MGI: J:162300

Service	Genotype	Price
	Heterozygous or wildtype for Tsc2<tm1Djk>

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Tsc2tm1Djk

Allele Symbol: Tsc2tm1Djk

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Tsc2tm1Djk related

Mammalian Phenotype Terms by Genotype

Genotype: Tsc2tm1Djk/Tsc2+involves: 129S4/SvJae * C57BL/6NCrl

behavior/neurological phenotype

nervous system phenotype

Genotype: Tsc2tm1Djk/Tsc2+involves: 129S4/SvJae * C57BL/6J

behavior/neurological phenotype

cellular phenotype

nervous system phenotype

Genotype: Tsc2tm1Djk/Tsc2+either: (involves: 129S4/SvJae * BALB/cJ) or (involves: 129S4/SvJae * Black Swiss) or (involves: 129S4/SvJae * C57BL/6J)

liver/biliary system phenotype

respiratory system phenotype

renal/urinary system phenotype

neoplasm

Genotype: Tsc2tm1Djk/Tsc2tm1Djkeither: (involves: 129S4/SvJae * BALB/cJ) or (involves: 129S4/SvJae * Black Swiss) or (involves: 129S4/SvJae * C57BL/6J)

mortality/aging

liver/biliary system phenotype

References

Additional References

Additional - Tsc2tm1Djk related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Tsc2^tm1Djk

Allele Symbol: Tsc2^tm1Djk

Genotype: Tsc2^tm1Djk related

Genotype: Tsc2^tm1Djk/Tsc2⁺
involves: 129S4/SvJae * C57BL/6NCrl

Genotype: Tsc2^tm1Djk/Tsc2⁺
involves: 129S4/SvJae * C57BL/6J

Genotype: Tsc2^tm1Djk/Tsc2⁺
either: (involves: 129S4/SvJae * BALB/cJ) or (involves: 129S4/SvJae * Black Swiss) or (involves: 129S4/SvJae * C57BL/6J)

Genotype: Tsc2^tm1Djk/Tsc2^tm1Djk
either: (involves: 129S4/SvJae * BALB/cJ) or (involves: 129S4/SvJae * Black Swiss) or (involves: 129S4/SvJae * C57BL/6J)

Additional - Tsc2^tm1Djk related