JAX Mouse Strain Datasheet - 004660

PWD/PhJ

Stock No:: 004660

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Overview

PWD/PhJ is an inbred mouse strain of the subspecies Mus musculus musculus. PWD/Ph mice exhibit differences that are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics. This strain is an excellent tool for mapping both single-gene (Mendelian) traits and quantitative trait loci (QTL) contributing to complex phenotypes.

Genetic overview

Genetic Background	Generation
	?+F9+F22 (01-JUL-16)

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Research Applications

Reproductive Biology Research
Research Tools

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Base Price

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$224.00 Domestic price for female

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Details

Detailed Description

PWD/PhJ is an inbred mouse strain of the subspecies Mus musculus musculus. M. m. musculus is estimated to have diverged approximately 1 million years ago from M. m. domesticus, the subspecies from which derives most of the genome of practically every laboratory mouse strain. However, the extent of polymorphism for randomly selected microsatellite markers between the two subspecies (70-80%) is nearly as great as between M. m. domesticus and Mus spretus (84%), which diverged about 3 million years ago (Montagutelli et al. 1991; Gergorova and Forejt 2000).

PWD/Ph mice exhibit differences are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics, including body mass, distribution of adipose tissue, serum concentrations of intermediary metabolites, susceptibility to type I diabetes and various behavioral traits (Gregorova and Forejt 2000).

Male, but not female, F1 hybrid offspring of crosses between mice of most laboratory strains and PWD/PhJ are sterile. Known exceptions are male (C3H x PWD)F1 and (CBA x PWD)F1 mice. The locus responsible for this difference in fertility of male PWD hybrids, called hybrid sterility 1 (symbol Hst1), has been mapped to a 580 kb region of M. m. domesticus Chr 17, approximately 8.4 cM distal to the centromere, wherein reside 10 potential candidate genes that are expressed in the testes (Forejt et al. 1991; Trachtulec et al. 1997). It has been estimated that no more than 3-4 loci contribute to the sterility phenotype of laboratory strain x M. m. musculus hybrid males (Gregorova and Forejt 2000).

Typing of PWD/Ph for 37 biochemical markers revealed numerous allelic differences with standard laboratory mouse strains (von Deimling et al. 1988). Electrophoretic alleles of 25 enzymes examined were shared between PWD and laboratory strains; nine of these markers were previously analyzed in wild-caught M. m. musculus mice, whose prevalent allele of all except one marker, Mod1, was fixed in PWD. PWD exhibited alleles at three loci that were uncommon among laboratory mice, but were identified in wild-caught M. m. musculus. Six esterase genes were identified in PWD that had also been detected in Mus molossinus but whose products were absent from laboratory strains. Unique alleles were identified in PWD for three enzymes.

Genome-wide microsatellite typing for 248 loci of PWD/Ph, PWK/Ph (distributed as PWK/PhJ, Stock No. 0003715) and/or PWB/Ph - very similar inbred strains derived from M. m. musculus pairs caught in the central Czech Republic - versus mice of three inbred laboratory strains revealed upto 80% of the loci to be polymorphic between at least one PW strain and at least one inbred strain analyzed.

The high level of both phenotypic variation and DNA sequence polymorphism between PWD/PhJ and inbred laboratory strains and the fertility of hybrid offspring of both sexes from crosses between PWD/PhJ and some laboratory strain mice make this strain an excellent tool for mapping both single-gene (Mendelian) traits and quantitative trait loci (QTL) contributing to complex phenotypes. Polymorphism at the RNA level permits investigation of monoallelic expression, e.g., from parentally imprinted genes (Forejt and Gregorova 1992; Forejt et al. 1999) or in cancer cell lines (Forejt et al. 1999). Sterility of male hybrid offspring of crosses between PWD and most inbred laboratory strains provides a tool for analysis of the genetic bases for interspecies hybrid sterility.

Development

PWD/Ph is an inbred mouse strain descended from a single pair of mice of the subspecies Mus musculus musculus caught in 1972 in the "Kunratice" locality near Prague, in the central part of the Czech Repubic. The founder mice were selected on the basis of their producing sterile male offspring in matings with C57BL/10J mice. The strain was maintained from its inception by sister-brother inbreeding (Gregorova and Forejt 2000). In July 2003, PWD/Ph mice, then at generation F81, were donated to The Jackson Laboratory by Dr. Jiri Forejt, at the Institute for Molecular Genetics, Academy of Sciences of the Czech Republic, Prague. The colony at The Jackson Laboratory is descended from 8 female and 2 male mice from Dr. Forejt's colony.

Selected References

Chubb C; Nolan C. 1987. Mouse hybrid sterility and testicular function. Biol Reprod 36(5):1343-8. PubMed: 3620567 MGI: J:109944
Gregorova S; Forejt J. 2000. PWD/Ph and PWK/Ph inbred mouse strains of Mus m. musculus subspecies--a valuable resource of phenotypic variations and genomic polymorphisms. Folia Biol (Praha) 46(1):31-41. PubMed: 10730880 MGI: J:62922
von Deimling OH; Forejt J; Wienker TF. 1988. Allelic profile at 37 biochemical loci of two inbred strains of the house mouse derived from wild Mus musculus musculus. Lab Anim 22(1):61-6. PubMed: 3352221 MGI: J:9097
von Deimling OH; Groning J; Gaa A. 1991. Genetic and biochemical characterization of a new chymotrypsin isozyme of the house mouse, CTRA-1. Biochem Genet 29(11-12):545-57. PubMed: 1820020 MGI: J:1547

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Genetics

Currently there are no related genes or alleles for this strain.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Reproductive Biology Research
- Fertility Defects
  - male progeny from outcrosses to inbred are sterile
Research Tools
- Genetics Research
  - Gene Mapping
  - Gene Mapping: Polymorphisms

Phenotype Information

Festing Inbred Strain Characteristics: PWD

References

Chubb C; Nolan C. 1987. Mouse hybrid sterility and testicular function. Biol Reprod 36(5):1343-8. PubMed: 3620567 MGI: J:109944
Gregorova S; Forejt J. 2000. PWD/Ph and PWK/Ph inbred mouse strains of Mus m. musculus subspecies--a valuable resource of phenotypic variations and genomic polymorphisms. Folia Biol (Praha) 46(1):31-41. PubMed: 10730880 MGI: J:62922
von Deimling OH; Forejt J; Wienker TF. 1988. Allelic profile at 37 biochemical loci of two inbred strains of the house mouse derived from wild Mus musculus musculus. Lab Anim 22(1):61-6. PubMed: 3352221 MGI: J:9097
von Deimling OH; Groning J; Gaa A. 1991. Genetic and biochemical characterization of a new chymotrypsin isozyme of the house mouse, CTRA-1. Biochem Genet 29(11-12):545-57. PubMed: 1820020 MGI: J:1547

Additional References

Forejt J; Gregorova S. 1992. Genetic analysis of genomic imprinting: an Imprintor-1 gene controls inactivation of the paternal copy of the mouse Tme locus. Cell 70(3):443-50. PubMed: 1322799 MGI: J:1689
Forejt J; Vincek V; Klein J; Lehrach H; Loudova-Mickova M. 1991. Genetic mapping of the t-complex region on mouse chromosome 17 including the Hybrid sterility-1 gene. Mamm Genome 1(2):84-91. PubMed: 1799792 MGI: J:558
Montagutelli X; Serikawa T; Guenet JL. 1991. PCR-analyzed microsatellites: data concerning laboratory and wild-derived mouse inbred strains. Mamm Genome 1(4):255-9. PubMed: 1794054 MGI: J:462
Storchova R; Gregorova S; Buckiova D; Kyselova V; Divina P; Forejt J. 2004. Genetic analysis of X-linked hybrid sterility in the house mouse. Mamm Genome 15(7):515-24. PubMed: 15366371 MGI: J:92309
Trachtulec Z; Mnukova-Fajdelova M; Hamvas RMJ; Gregorova S; Mayer WE; Lehrach HR; Vincek V; Forejt J; Klein J. 1997. Isolation of candidate hybrid sterility 1 genes by cDNA selection in a 1.1 megabase pair region on mouse chromosome 17. Mamm Genome 8(5):312-6. PubMed: 9107673 MGI: J:39622
Vied C; Ray S; Badger CD; Bundy JL; Arbeitman MN; Nowakowski RS. 2016. Transcriptomic analysis of the hippocampus from six inbred strains of mice suggests a basis for sex-specific susceptibility and severity of neurological disorders. J Comp Neurol 524(13):2696-710. PubMed: 26917114 MGI: J:235042

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The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Overview

Genetic overview

Research Applications

Base Price

Details

Detailed Description

Development

Selected References

Genetics

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Phenotype Information

References

Additional References

Technical Support

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Appearance

Citation

Animal Health Reports

Pricing & Availability

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability