PWD/PhJ is an inbred mouse strain of the subspecies Mus musculus musculus. PWD/Ph mice exhibit differences that are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics. This strain is an excellent tool for mapping both single-gene (Mendelian) traits and quantitative trait loci (QTL) contributing to complex phenotypes.Read More +
PWD/PhJ is an inbred mouse strain of the subspecies Mus musculus musculus. M. m. musculus is estimated to have diverged approximately 1 million years ago from M. m. domesticus, the subspecies from which derives most of the genome of practically every laboratory mouse strain. However, the extent of polymorphism for randomly selected microsatellite markers between the two subspecies (70-80%) is nearly as great as between M. m. domesticus and Mus spretus (84%), which diverged about 3 million years ago (Montagutelli et al. 1991; Gergorova and Forejt 2000).
PWD/Ph mice exhibit differences are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics, including body mass, distribution of adipose tissue, serum concentrations of intermediary metabolites, susceptibility to type I diabetes and various behavioral traits (Gregorova and Forejt 2000).
Male, but not female, F1 hybrid offspring of crosses between mice of most laboratory strains and PWD/PhJ are sterile. Known exceptions are male (C3H x PWD)F1 and (CBA x PWD)F1 mice. The locus responsible for this difference in fertility of male PWD hybrids, called hybrid sterility 1 (symbol Hst1), has been mapped to a 580 kb region of M. m. domesticus Chr 17, approximately 8.4 cM distal to the centromere, wherein reside 10 potential candidate genes that are expressed in the testes (Forejt et al. 1991; Trachtulec et al. 1997). It has been estimated that no more than 3-4 loci contribute to the sterility phenotype of laboratory strain x M. m. musculus hybrid males (Gregorova and Forejt 2000).
Typing of PWD/Ph for 37 biochemical markers revealed numerous allelic differences with standard laboratory mouse strains (von Deimling et al. 1988). Electrophoretic alleles of 25 enzymes examined were shared between PWD and laboratory strains; nine of these markers were previously analyzed in wild-caught M. m. musculus mice, whose prevalent allele of all except one marker, Mod1, was fixed in PWD. PWD exhibited alleles at three loci that were uncommon among laboratory mice, but were identified in wild-caught M. m. musculus. Six esterase genes were identified in PWD that had also been detected in Mus molossinus but whose products were absent from laboratory strains. Unique alleles were identified in PWD for three enzymes.
Genome-wide microsatellite typing for 248 loci of PWD/Ph, PWK/Ph (distributed as PWK/PhJ, Stock No. 0003715) and/or PWB/Ph - very similar inbred strains derived from M. m. musculus pairs caught in the central Czech Republic - versus mice of three inbred laboratory strains revealed upto 80% of the loci to be polymorphic between at least one PW strain and at least one inbred strain analyzed.
The high level of both phenotypic variation and DNA sequence polymorphism between PWD/PhJ and inbred laboratory strains and the fertility of hybrid offspring of both sexes from crosses between PWD/PhJ and some laboratory strain mice make this strain an excellent tool for mapping both single-gene (Mendelian) traits and quantitative trait loci (QTL) contributing to complex phenotypes. Polymorphism at the RNA level permits investigation of monoallelic expression, e.g., from parentally imprinted genes (Forejt and Gregorova 1992; Forejt et al. 1999) or in cancer cell lines (Forejt et al. 1999). Sterility of male hybrid offspring of crosses between PWD and most inbred laboratory strains provides a tool for analysis of the genetic bases for interspecies hybrid sterility.
In 2019-2020, researchers at The Jackson Laboratory discovered this inbred strain contains the Trem2S148E allele - a naturally occurring variant at position 48351151-48351152 on Chr 17 (rs108080490 and rs107649577; Ensembl GRCm38.p6). This TC to GA transition results in a serine to glutamic acid substitution at amino acid 148 (S148E).
PWD/Ph is an inbred mouse strain descended from a single pair of mice of the subspecies Mus musculus musculus caught in 1972 in the "Kunratice" locality near Prague, in the central part of the Czech Repubic. The founder mice were selected on the basis of their producing sterile male offspring in matings with C57BL/10J mice. The strain was maintained from its inception by sister-brother inbreeding (Gregorova and Forejt 2000). In July 2003, PWD/Ph mice, then at generation F81, were donated to The Jackson Laboratory by Dr. Jiri Forejt, at the Institute for Molecular Genetics, Academy of Sciences of the Czech Republic, Prague. The colony at The Jackson Laboratory is descended from 8 female and 2 male mice from Dr. Forejt's colony.
Currently there are no related genes or alleles for this strain.
These mice are still very wild after 80 generations of inbreeding, and special care is needed to prevent their escape during handling. PWD/Ph mice breed well. Females produce their first litters at about 8 weeks of age and will give birth approximately every 4 weeks. The average litter size is 7. Females continue breeding until at least 8 months old; the average number of litters produced during their reproductive lifetimes has been reported to be 6-7.
When using the PWD/PhJ mouse strain in a publication, please include JAX stock #004660 in your Materials and Methods section.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.