B6.Cg-Tg(TIE2GFP)287Sato/1J

Stock number: 004659
Research areas: Cardiovascular Research, Developmental Biology Research, Endocrine Deficiency Research, Research Tools

Description

This strain has been discontinued. Please refer to STOCK Tg(TIE2GFP)287Sato/J Stock No. 003658.

Detailed description

This strain expresses Green Fluorescent Protein (GFP) under the direction of the endothelial-specific receptor tyrosine kinase (Tek, formerly, Tie2) promoter. Endothelial cells expressing GFP can be visualized via fluorescent microscopy or purified by FACS.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

This strain originated on an FVB/N background, and was backcrossed to C57BL/6J for 5 generations using a speed congenic protocol. The C57BL/6J component of this speed congenic background is estimated at >95% via genome scanning.

Allele

Symbol: Tg(TIE2GFP)287Sato
Name: transgene insertion 287, Thomas N Sato
MGI accession ID: MGI:3056937
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(TIE2GFP)287Sato
Marker name: transgene insertion 287, Thomas N Sato
Chromosome: UN
Strain of origin: Not Applicable
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Blood vessel endothelial cells fluoresce green allowing the visualization of all vessels under fluorescent microscopy or purification of endothelial cells by FACS.
Molecular note: The transgene contains Green Fluorescent Protein (GFP) under the control of the endothelial specific receptor tyrosine kinase (Tek, previous symbol Tie2) promoter.
General note: Endothelial cells in transgenic mice express GFP, and can be visualized via fluorescent microscopy or purified by FACS.